A critical variable: Reaction rates, material properties, and biological processes can be strongly affected by a metal ion’s oxidation state, coordination form, and concentration (even for the same metal, behavior can be completely different under different ligation/oxidation states).
Ni²⁺–agarose beads (typically Ni-NTA/Ni-IDA) capture recombinant proteins via coordination between multiple immobilized chelation sites and the imidazole rings of His tags.
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