Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
Ni-agarose beads are typically blue due to coordination complexes between Ni²⁺ and the immobilized chelating ligand. In purification systems containing reducing agents, metal reduction or drastic changes to the coordination environment can shift the bead color from blue to brown or black.
Updated Nov 202512 min read✓ Reviewed
Related topics
Techniques often explored alongside immunological experiments.
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