This SOP is intended for the standardized application of SYTO 9 and PI combined live/dead staining in microbial viability evaluation. It is suitable for suspended bacterial samples such as Escherichia coli and Staphylococcus aureus, and can also be extended to membrane integrity stratification ...
This procedure is intended for the standardized use of the two far-red nuclear dyes DRAQ5 and DRAQ7. It is applicable to live-cell nuclear labeling, dead-cell identification, nuclear counterstaining of fixed samples, and immunofluorescence-associated detection on fluorescence microscopy, ...
This protocol has been successfully adapted to different cell types, including fibroblasts, PC12 cells, and embryonic neurons, with different Fura-2 AM concentrations corresponding to different cell densities .
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