Topic: Urea

Articles by Topic "Urea"

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  1. Comparing Guanidine Hydrochloride and Urea for Inclusion-Body Solubilization Recombinant proteins frequently accumulate as inclusion bodies in prokaryotic hosts and must be solubilized under denaturing conditions before refolding to regain native structure and activity. Urea and Guanidine Hydrochloride (GdnHCl) are the two workhorse denaturants: urea is cost-effective ...
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