Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
Recombinant proteins frequently accumulate as inclusion bodies in prokaryotic hosts and must be solubilized under denaturing conditions before refolding to regain native structure and activity. Urea and Guanidine Hydrochloride (GdnHCl) are the two workhorse denaturants: urea is cost-effective ...
Updated Oct 202512 min read✓ Reviewed
Related topics
Techniques often explored alongside immunological experiments.
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