Goat Anti-Human IgG (FITC) (Ready to use) - Secondary antibody, specific to Human IgG

Cat. No.: Ab220089

GRADE & PURITY Validated, ExactAb™, See COA

Application

Flow Cytometry, IF/ICC

Host species

Goat

Reactive species

Human

Isotype

Goat IgG

Conjugation

FITC

Specifications

Product Name

Goat Anti-Human IgG (FITC) (Ready to use) - Secondary antibody, specific to Human IgG

Synonyms

Goat Anti-Human IgG H&L Antibody | Goat Anti Human IgG H&L Antibody | Anti-Human IgG H&L Antibody | Anti Human IgG H&L Antibody | Human IgG H&L Antibody | Goat Anti-Human IgG Antibody | Goat Anti Human IgG Antibody | Anti-Human IgG Antibody | Anti Human I

Specifications & Purity

ExactAb™, Validated, See COA

Biochemical and Physiological Mechanisms

Fluorescent secondary antibodies recognize IgG heavy chains and all classes of immunoglobulin light chains from human. Ready-to-use secondary antibody concentrations have been optimized and tested by immunofluorescent staining with Flow Cytometry and IF/I

Host species

Goat

Specificity

Human IgG

Conjugation

FITC

Excitation (nm)

Blue Laser(488nm)

Emission(nm)

517nm

Ex/Em(nm)

Ex:498nm;Em:517nm

Grade

ExactAb™, Validated

Purification Method

antigen affinity chromatography

Concentration

See COA

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

This product can be stored at 2-8°C for 12 months. Do not freeze. Store in the dark.

Images

IF/ICC: Goat Anti-Human IgG (FITC) (Ready to use) (Ab220089)

Goat Anti-Human IgG (FITC) (Ready to use) (Ab220089) - IF/ICC
IF analysis of EpCAM (green) in HT-29 cells. The cells were fixed with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Citatuzumab (anti-EpCAM) (Ab209902) at 1/1000 dilution in blocking buffer overnight at 4°C. Then incubated with Goat Anti-Human IgG (FITC) (Ready to use) (Ab220089) at 1mL/well for 1 hour at room temperature in the dark (green). A 24-well cell culture plate was used for this experiment. PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Flow Cytometry: Goat Anti-Human IgG (FITC) (Ready to use) (Ab220089)

Goat Anti-Human IgG (FITC) (Ready to use) (Ab220089) - Flow Cytometry
Flow Cytometry analysis of HT-29 cells labelling EpCAM (red) with Citatuzumab (anti-EpCAM) (Ab209902). Goat Anti-Human IgG (FITC) (Ready to use) (Ab220089) at 100 μL/well was used as the secondary antibody. Blue - Isotype control, Human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• IF/ICC:

  Use at an assay dependent volume.;

• Flow Cytometry:

  100μL/test