Goat Anti-Mouse IgG H&L (AF594) - Secondary antibody, specific to Mouse IgG

Cat. No.: Ab223359

GRADE & PURITY Validated, ExactAb™, Azide Free, Pre-adsorbed, 1.0 mg/mL

Application

Flow Cytometry, IF/ICC

Host species

Goat

Isotype

Goat IgG

Conjugation

AF594

Overview

Goat Anti-Mouse IgG H&L Antibody, Secondary antibody, AF594 conjugated, could be used for IF/ICC and Flow Cytometry.

Specifications

Product Name

Goat Anti-Mouse IgG H&L (AF594) - Secondary antibody, specific to Mouse IgG

Specifications & Purity

ExactAb™, Validated, Azide Free, Pre-adsorbed, 1.0 mg/mL

Host species

Goat

Specificity

Mouse IgG

Immunogen

Mouse IgG

Conjugation

AF594

Excitation (nm)

Yellow-Green Laser(594nm)

Emission(nm)

618nm

Ex/Em(nm)

Ex:590nm;Em:618nm

Grade

Azide Free, ExactAb™, Pre-adsorbed, Validated

Purification Method

Antigen affinity purified

Concentration

1.0 mg/mL

Storage and Shipping

Storage

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20°C. Avoid freeze/thaw cycle. Protect from light.

Images

IF/ICC: Goat Anti-Mouse IgG H&L (AF594) (Ab223359)

Goat Anti-Mouse IgG H&L (AF594) (Ab223359) - IF/ICC 

IF analysis of EpCAM (red) in HT-29 cells. The cells were fixed with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Catumaxomab (anti-CD3&EpCAM) (Ab175489) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (AF594) (Ab223359) at a dilution of 1/500 for 1 hour at room temperature in the dark (red). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with Hoechst 33342 (B113845) (blue) at a dilution of 1/1000 for 5 minutes in the dark. Images were taken on the confocal laser scanning microscope.

Flow Cytometry: Goat Anti-Mouse IgG H&L (AF594) (Ab223359)

Goat Anti-Mouse IgG H&L (AF594) (Ab223359) - Flow Cytometry 

Flow Cytometry analysis of Jurkat cells labelling CD45 (red) with Recombinant CD45 Antibody (Ab210608). Goat Anti-Mouse IgG H&L (AF594) (Ab223359) at a dilution of 1/900 was used as the secondary antibody. Blue - Isotype control, Mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  1:100-1:2000 ;

• IF/ICC:

  1:500 ;