Goat Anti-Mouse IgG H&L (TRITC) (Ready to use) - Secondary antibody, specific to Mouse IgG

Cat. No.: Ab220003

GRADE & PURITY Validated, ExactAb™, See COA

Application

Flow Cytometry, IF/ICC

Host species

Goat

Reactive species

Mouse

Isotype

Goat IgG

Conjugation

TRITC

Overview

Fluorescent secondary antibodies recognize IgG heavy chains and all classes of immunoglobulin light chains from mouse. Ready-to-use secondary antibody concentrations have been optimized and tested by immunofluorescent staining with Flow Cytometry and IF/ICC. No calculations and dilutions are required. Just add an assay dependent volume to stain your cells, and you're ready for the next step in your experiment.

Specifications

Product Name

Goat Anti-Mouse IgG H&L (TRITC) (Ready to use) - Secondary antibody, specific to Mouse IgG

Synonyms

Goat Anti-Mouse IgG H&L Antibody | Goat Anti Mouse IgG H&L Antibody | Anti-Mouse IgG H&L Antibody | Anti Mouse IgG H&L Antibody | Mouse IgG H&L Antibody | Goat Anti-Mouse IgG Antibody | Goat Anti Mouse IgG Antibody | Anti-Mouse IgG Antibody | Anti Mouse I

Specifications & Purity

ExactAb™, Validated, See COA

Host species

Goat

Specificity

Mouse IgG

Immunogen

Mouse IgG

Conjugation

TRITC

Excitation (nm)

Green Laser(532nm)

Emission(nm)

576nm

Ex/Em(nm)

Ex:557nm;Em:576nm

Grade

ExactAb™, Validated

Purification Method

antigen affinity chromatography

Concentration

See COA

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

This product can be stored at 2-8°C for 12 months. Do not freeze. Store in the dark.

Images

IF/ICC : Goat Anti-Mouse IgG H&L (TRITC) (Ready to use) (Ab220003)

Goat Anti-Mouse IgG H&L (TRITC) (Ready to use) (Ab220003) - IF/ICC
IF analysis of EpCAM (red) in HT-29 cells. The cells were fixed with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes, permeabilized with 0.3%  Triton X-100 (I743383) for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with 1 μg/mL Catumaxomab (anti-CD3&EpCAM) (Ab175489) in blocking buffer overnight at 4°C. Then incubated with Goat Anti-Mouse IgG H&L (TRITC) (Ready to use) (Ab220003) at 1mL/well for 1 hour at room temperature in the dark (red). A 24-well cell culture plate was used for this experiment. PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Flow Cytometry: Goat Anti-Mouse IgG H&L (TRITC) (Ready to use) (Ab220003)

Goat Anti-Mouse IgG H&L (TRITC) (Ready to use) (Ab220003) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells labelling CD45 (red) with Recombinant CD45 Antibody (Ab210608). Goat Anti-Mouse IgG H&L (TRITC) (Ready to use) (Ab220003) at 100 μL/well was used as the secondary antibody. Blue - Isotype control, Mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• IF/ICC:

  Use at an assay dependent volume.;

• Flow Cytometry:

  100μL/test