GRADE & PURITYValidated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.See COA
Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20°C. Avoid freeze/thaw cycle. Protect from light.
Images
Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (Ab176449) - Western Blot All lanes: beta Actin Antibody (Ab090999) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (Ab176449) at 1/20000 dilution Detection of alkaline phosphatase (AP) was performed using BCIP/NBT (B494228) after 1hour incubated at room temperature.
Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 0.6 s
Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (Ab176449) - ELISA Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (Ab176449) was tested by direct ELISA. The plate was coated with 0.5 µg/mL (100 µL/well) Rabbit IgG (Ab170215). A serial dilution of Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (Ab176449) was used as the secondary antibody. Detection of alkaline phosphatase (AP) was performed using pNPP (P494193) after 30 minutes incubated at room temperature.
Application
Application
Dilution info
ELISA
1:1000-1:20000
WB
1:2000-1:40000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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