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| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
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Moligand™, 10mM in DMSO Moligand™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at -80°C Ships Dry ice packs + Cold packs Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 15 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Information
GW9662 is a selective PPAR antagonist forPPARγwithIC50of 3.3 nM in a cell-free assay, with at least 10- to 600-fold functional selectivity in cells with PPARγ versus PPARα and PPARδ.
In vitro
GW9662 binds to Cys(285) on PPARgamma which is conserved among all three PPARs. GW9662 acts as an antagonist of PPARgamma which is confirmed in an assay of adipocyte differentiation inhibition. GW9662 prevents activation of PPARγ and inhibits growth of human mammary tumour cell lines (MCF7, MDA-MB-468, MDA-MB-231) with IC50 of 20 μM-30 μM, suggesting either the existence of PPARγ agonistic properties of GW9662 or growth-inhibitory mechanisms independent of PPARγ. Co-treatment with both Rosiglitazone (50 μM) and GW9662 (10 μM) results in statistically lower viable cell numbers after 7 days in MDA-MB-231 cells. PPARγ1 ligands could suppress RANKL-induced osteoclast formation in primary murine myeloid (BMs) and RAW264.7 cells. Importantly, suppression by these ligands is reversed in a concentration-dependent fashion with GW 9662 (2 μM). GW 9662 (2 μM) blocks IL-4 suppression of osteoclast formation in BMs. GW 9662 (1 μM) blocks RANKL activation of NF-κB in RAW264.7 cells. GW9662 (10 μM) inhibits hormone- and agonist-induced adipogenesis of primary preadipocytes from patients with thyroid eye disease.
In vivo
Pretreatment with LPS (1 mg/kg i.p.) significantly attenuates all markers of renal injury and dysfunction caused by ischemia/reperfusion (I/R) injury in rats. Most notably, GW9662 (1 mg/kg i.p.) abolishes the protective effects of LPS.
Cell Data
cell lines:PHA-stimulated PBMC or PM-1 cells
Concentrations:10 μM
Incubation Time:10 days
Powder Purity:≥99%
| Isomeric SMILES | C1=CC=C(C=C1)NC(=O)C2=C(C=CC(=C2)[N+](=O)[O-])Cl |
|---|---|
| WGK Germany | 3 |
| Molecular Weight | 276.68 |
| Reaxy-Rn | 2749539 |
| Reaxys-RN_link_address | https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=2749539&ln= |
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
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| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
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| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
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| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
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| Solubility | Solubility (25°C) In vitro Water: 29 mg/mL (198.43 mM); DMSO: Insoluble; |
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| 1. Ling Yang, Linzi Li, Qian Lu, Lingfeng Li, Chun Xie, Fakun Jiang, Hongbing Li, Ai Zhao, Qian Wang, Wenyong Xiong. (2023) Alisol B blocks the development of HFD-induced obesity by triggering the LKB1-AMPK signaling in subcutaneous adipose tissue. EUROPEAN JOURNAL OF PHARMACOLOGY, [PMID:37536624] [10.1016/j.ejphar.2023.175942] |
| 2. Sun Penghao, Wang Mengli, Liu Yong-Xin, Li Luqi, Chai Xuejun, Zheng Wei, Chen Shulin, Zhu Xiaoyan, Zhao Shanting. (2023) High-fat diet-disturbed gut microbiota-colonocyte interactions contribute to dysregulating peripheral tryptophan-kynurenine metabolism. Microbiome, 11 (1): (1-20). [PMID:37468922] [10.1186/s40168-023-01606-x] |
| 3. Yushuang Liu, Hanshu Zhao, Nan Chen, Yuelong Li, Zeyang Zheng, Zhiyu Sun, Zhongling Zhang. (2023) PHLDA1 knockdown alleviates mitochondrial dysfunction and endoplasmic reticulum stress-induced neuronal apoptosis via activating PPARγ in cerebral ischemia-reperfusion injury. BRAIN RESEARCH BULLETIN, [PMID:36681251] [10.1016/j.brainresbull.2023.01.007] |
| 4. Shuang Luo, Jinrong He, Shaowei Huang, Xiaojing Wang, Yulin Su, Yanyang Li, Yanping Chen, Guanghua Yang, Bin Huang, Shaoju Guo, Lian Zhou, Xia Luo. (2022) Emodin targeting the colonic metabolism via PPARγ alleviates UC by inhibiting facultative anaerobe. PHYTOMEDICINE, [PMID:35728384] [10.1016/j.phymed.2022.154106] |
| 5. Zhenxing Liang, Ying Chen, Zheng Wang, Xue Wu, Chao Deng, Changyu Wang, Wenwen Yang, Ye Tian, Shaofei Zhang, Chenxi Lu, Yang Yang. (2021) Protective effects and mechanisms of psoralidin against adriamycin-induced cardiotoxicity. Journal of Advanced Research, [PMID:36100330] [10.1016/j.jare.2021.12.007] |
| 6. Zhishen Xie, Jianping Zhao, Hui Wang, Yali Jiang, Qiaoling Yang, Yu Fu, Huahui Zeng, Christian Hölscher, Jiangyan Xu, Zhenqiang Zhang. (2020) Magnolol alleviates Alzheimer's disease-like pathology in transgenic C. elegans by promoting microglia phagocytosis and the degradation of beta-amyloid through activation of PPAR-γ. BIOMEDICINE & PHARMACOTHERAPY, [PMID:32000045] [10.1016/j.biopha.2020.109886] |
| 7. Ningning He, Mengjie Mu, Xiaofang Li, Qingyuan Hao, Kaiwei Chen, Xinnan Zhao, Yang Sun, Haoyu Wang, Zhinan Wu, Hewei Liang, Mengmeng Wang, Liang Xiao, Tao Yu, Zhi-Peng Wang, Jixing Peng, Yuanqiang Zou, Shangyong Li. (2025) Gut Commensal Antibiotic-Resistant Parabacteroides goldsteinii Ameliorates Mouse Colitis through Valine–Isobutyrate Metabolism. Research, [PMID:40948937] [10.34133/research.0867] |
| 8. Yang Yang, Yue Tao, Rongyi Yang, Xiaodong Yi, Guanyu Zhong, Yanyan Gu, Ying Zhang. (2024) Ca2+ homeostasis imbalance induced by Pparg: A key factor in di (2-ethylhexyl) phthalate (DEHP)-induced cardiac dysfunction in zebrafish larvae. SCIENCE OF THE TOTAL ENVIRONMENT, [PMID:38281650] [10.1016/j.scitotenv.2024.170436] |
| 9. Wei Guo, Jian Han, Shengmin Wu, Xiongjie Shi, Qiangwei Wang, Bingsheng Zhou. (2019) Bis(2-ethylhexyl)-2,3,4,5-tetrabromophthalate Affects Lipid Metabolism in Zebrafish Larvae via DNA Methylation Modification. ENVIRONMENTAL SCIENCE & TECHNOLOGY, [PMID:31804803] [10.1021/acs.est.9b05796] |
| 10. Yingying Yu, Kuankuan Yuan, Difei Tong, Weixia Zhang, Weishang Zhou, Weifeng Li, Wei Shi, Weidong Yang, Xinguo Zhao, Guangxu Liu. (2025) Perfluorooctanoic acid (PFOA) disrupts cardiac performance of thick-shell mussel by inserting into the binding pocket of proliferator-activated receptor gamma and thereby causing lipid metabolism disorders. ENVIRONMENTAL POLLUTION, [PMID:40339884] [10.1016/j.envpol.2025.126369] |
| 11. Hongyi Xian, Ruobing Bai, Yu Feng, Shiyue Tang, Zhiming Li, Chudan Gao, Kaiwei Li, Boxuan Liang, Yizhou Zhong, Yuji Huang, Hao Li, Yanhong Deng, Xiaohong Yang, Xiyun Huang, Xiaoqing Chen, Xingfen Yang, Zhenlie Huang. (2025) Aged polylactic acid microplastics exacerbate lipid metabolism disorders and cardiac dysfunction via PPARγ activation in zebrafish: A comparative study with polymers and oligomers. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, [PMID:41187537] [10.1016/j.ecoenv.2025.119337] |
| 12. Yinye Yao, Chunjing Xu, Yongfu Shao, Xiaoqing Xu, Qian Yin, Xinyuan Cao, Meifang Zheng, Duqin Zhao, Wangye Zheng, Jiaojiao Ni, Jieer Ying, Yunben Yang. (2025) Linoleic Acid Potentiates Response to Chemotherapy in Biliary Tract Cancer Through RARγ Activation. FASEB JOURNAL, 39 (22): (e71232). [PMID:41229376] [10.1096/fj.202502665R] |
| 13. Jinzhi Ren, Ying Peng, Chenyang Sun, Yining Zhang, Jianjun Li, Simin Xia, Caixia Gao, Yimeng Wang, Xiangping Nie. (2025) New Approach Methodologies (NAMs) reveal triphenyl phosphate (TPP) disrupts lipid metabolism in estuarine fish via PPARγ activation: A concentration-dependent transcriptomic and molecular docking study. Environmental Chemistry and Ecotoxicology, [PMID:] [10.1016/j.enceco.2025.11.037] |
| 14. Di Yang, Lianhui Li, Kailai Zang, Wanyong Ma, Yuling Yang, Yani Sun, Bingqiang Zhang, Zunshuang Gong, Mingkang Yu, Qiyuan Du, Xiaokun Liu, Zhe Wang, Qiyue Xu, Ning Li. (2025) EVA1A Regulates Hepatic Lipid Homeostasis by Modulating CD36 Expression and Its Palmitoylation. Research, [PMID:41306774] [10.34133/research.1001] |
| 15. Yuchen Zheng, Zijia Liu, Zijie Zhao, Chao Sheng, Wenzhuo Li, Duo Tang, Yunlu Ning, Renhang Zhou, Ao Li, Zhixiang Zhou. (2026) Polystyrene nanoplastics promote adipogenesis by stimulating nuclear translocation of PPARγ. FOOD AND CHEMICAL TOXICOLOGY, [PMID:41763287] [10.1016/j.fct.2026.116030] |
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