GW9662 - Moligand™, 10mM in DMSO , CAS No.22978-25-2

CAS: 22978-25-2 Cat. No.: G407990 Molecular Weight: 276.68 EC Number: 636-590-7
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GRADE & PURITY Moligand™ ? Moligand™ — Aladdin's line of ligands and bioactive small molecules. Use for receptor, pathway, and binding studies needing defined small-molecule tools. 10mM in DMSO
Synonyms
Benzamide, 2-chloro-5-nitro-N-phenyl-
Storage
Store at -80°C
Shipped In
Dry ice packs + Cold packs
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Size
Status
Price
Qty
1ml
G407990-1ml
2

$149.90

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Why this grade

Moligand™, 10mM in DMSO Moligand™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

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Storage & shipping

Store at -80°C Ships Dry ice packs + Cold packs Check lot-specific COA for exact specifications.

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Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

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Literature proof

Cited in 15 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Information

GW9662 is a selective PPAR antagonist forPPARγwithIC50of 3.3 nM in a cell-free assay, with at least 10- to 600-fold functional selectivity in cells with PPARγ versus PPARα and PPARδ.
In vitro

GW9662 binds to Cys(285) on PPARgamma which is conserved among all three PPARs. GW9662 acts as an antagonist of PPARgamma which is confirmed in an assay of adipocyte differentiation inhibition. GW9662 prevents activation of PPARγ and inhibits growth of human mammary tumour cell lines (MCF7, MDA-MB-468, MDA-MB-231) with IC50 of 20 μM-30 μM, suggesting either the existence of PPARγ agonistic properties of GW9662 or growth-inhibitory mechanisms independent of PPARγ. Co-treatment with both Rosiglitazone (50 μM) and GW9662 (10 μM) results in statistically lower viable cell numbers after 7 days in MDA-MB-231 cells. PPARγ1 ligands could suppress RANKL-induced osteoclast formation in primary murine myeloid (BMs) and RAW264.7 cells. Importantly, suppression by these ligands is reversed in a concentration-dependent fashion with GW 9662 (2 μM). GW 9662 (2 μM) blocks IL-4 suppression of osteoclast formation in BMs. GW 9662 (1 μM) blocks RANKL activation of NF-κB in RAW264.7 cells. GW9662 (10 μM) inhibits hormone- and agonist-induced adipogenesis of primary preadipocytes from patients with thyroid eye disease.

In vivo

Pretreatment with LPS (1 mg/kg i.p.) significantly attenuates all markers of renal injury and dysfunction caused by ischemia/reperfusion (I/R) injury in rats. Most notably, GW9662 (1 mg/kg i.p.) abolishes the protective effects of LPS.
Cell Data

cell lines:PHA-stimulated PBMC or PM-1 cells

Concentrations:10 μM

Incubation Time:10 days

Powder Purity:≥99%

Specifications

Synonyms
Benzamide, 2-chloro-5-nitro-N-phenyl-
Specifications & Purity
Moligand™, 10mM in DMSO
Biochemical and Physiological Mechanisms
GW9662 is a selective PPAR antagonist for PPARγ with IC50 of 3.3 nM in a cell-free assay, with at least 10- to 600-fold functional selectivity in cells with PPARγ versus PPARα and PPARδ.
Storage
Store at -80°C
Shipped In
Dry ice packs + Cold packs
This product requires cold chain shipping. Ground and other economy services are not available.
Grade
Moligand™
Action Type
ANTAGONIST
Names and Identifiers
Isomeric SMILES C1=CC=C(C=C1)NC(=O)C2=C(C=CC(=C2)[N+](=O)[O-])Cl
WGK Germany 3
Molecular Weight 276.68
Reaxy-Rn 2749539
Reaxys-RN_link_address https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=2749539&ln=

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
CYP1A2 Tchem Cytochrome P450 1A2 (1 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
CYP2C19 Tchem Cytochrome P450 2C19 (1 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
RORC Tchem Nuclear receptor ROR-gamma (1 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
PPARG Tclin Peroxisome proliferator-activated receptor gamma (2 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Chemical and Physical Properties
SolubilitySolubility (25°C) In vitro Water: 29 mg/mL (198.43 mM); DMSO: Insoluble;  
Citations of This Product
References
1. Ling Yang, Linzi Li, Qian Lu, Lingfeng Li, Chun Xie, Fakun Jiang, Hongbing Li, Ai Zhao, Qian Wang, Wenyong Xiong.  (2023)  Alisol B blocks the development of HFD-induced obesity by triggering the LKB1-AMPK signaling in subcutaneous adipose tissue.  EUROPEAN JOURNAL OF PHARMACOLOGY,      [PMID:37536624] [10.1016/j.ejphar.2023.175942]
2. Sun Penghao, Wang Mengli, Liu Yong-Xin, Li Luqi, Chai Xuejun, Zheng Wei, Chen Shulin, Zhu Xiaoyan, Zhao Shanting.  (2023)  High-fat diet-disturbed gut microbiota-colonocyte interactions contribute to dysregulating peripheral tryptophan-kynurenine metabolism.  Microbiome,  11  (1): (1-20).  [PMID:37468922] [10.1186/s40168-023-01606-x]
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8. Yang Yang, Yue Tao, Rongyi Yang, Xiaodong Yi, Guanyu Zhong, Yanyan Gu, Ying Zhang.  (2024)  Ca2+ homeostasis imbalance induced by Pparg: A key factor in di (2-ethylhexyl) phthalate (DEHP)-induced cardiac dysfunction in zebrafish larvae.  SCIENCE OF THE TOTAL ENVIRONMENT,      [PMID:38281650] [10.1016/j.scitotenv.2024.170436]
9. Wei Guo, Jian Han, Shengmin Wu, Xiongjie Shi, Qiangwei Wang, Bingsheng Zhou.  (2019)  Bis(2-ethylhexyl)-2,3,4,5-tetrabromophthalate Affects Lipid Metabolism in Zebrafish Larvae via DNA Methylation Modification.  ENVIRONMENTAL SCIENCE & TECHNOLOGY,      [PMID:31804803] [10.1021/acs.est.9b05796]
10. Yingying Yu, Kuankuan Yuan, Difei Tong, Weixia Zhang, Weishang Zhou, Weifeng Li, Wei Shi, Weidong Yang, Xinguo Zhao, Guangxu Liu.  (2025)  Perfluorooctanoic acid (PFOA) disrupts cardiac performance of thick-shell mussel by inserting into the binding pocket of proliferator-activated receptor gamma and thereby causing lipid metabolism disorders.  ENVIRONMENTAL POLLUTION,      [PMID:40339884] [10.1016/j.envpol.2025.126369]
11. Hongyi Xian, Ruobing Bai, Yu Feng, Shiyue Tang, Zhiming Li, Chudan Gao, Kaiwei Li, Boxuan Liang, Yizhou Zhong, Yuji Huang, Hao Li, Yanhong Deng, Xiaohong Yang, Xiyun Huang, Xiaoqing Chen, Xingfen Yang, Zhenlie Huang.  (2025)  Aged polylactic acid microplastics exacerbate lipid metabolism disorders and cardiac dysfunction via PPARγ activation in zebrafish: A comparative study with polymers and oligomers.  ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,      [PMID:41187537] [10.1016/j.ecoenv.2025.119337]
12. Yinye Yao, Chunjing Xu, Yongfu Shao, Xiaoqing Xu, Qian Yin, Xinyuan Cao, Meifang Zheng, Duqin Zhao, Wangye Zheng, Jiaojiao Ni, Jieer Ying, Yunben Yang.  (2025)  Linoleic Acid Potentiates Response to Chemotherapy in Biliary Tract Cancer Through RARγ Activation.  FASEB JOURNAL,  39  (22): (e71232).  [PMID:41229376] [10.1096/fj.202502665R]
13. Jinzhi Ren, Ying Peng, Chenyang Sun, Yining Zhang, Jianjun Li, Simin Xia, Caixia Gao, Yimeng Wang, Xiangping Nie.  (2025)  New Approach Methodologies (NAMs) reveal triphenyl phosphate (TPP) disrupts lipid metabolism in estuarine fish via PPARγ activation: A concentration-dependent transcriptomic and molecular docking study.  Environmental Chemistry and Ecotoxicology,      [PMID:] [10.1016/j.enceco.2025.11.037]
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