H7 , CAS No.2803681-14-1

CAS: 2803681-14-1 Cat. No.: H1452440 Formula: C₇₀H₆₆N₈O₄SSi₂ Molecular Weight: 1171.56

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Overview

H7 is a second near-infrared (NIR-II) fluorophore.

Specifications

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Names and Identifiers

• Molecular Weight: 1171.56

Application Protocols

Item-specific facts (from Product Data)

• No tested applications or protocols are provided.

General protocol templates (non–item-specific)

• Preparation of stock solutions: Dissolve in dry DMSO or appropriate solvent to 10–100 mM; vortex/sonicate; sterile-filter (0.22 µm) if used in cell culture; aliquot to avoid freeze–thaw.
• In vitro enzyme assay dosing: Prepare serial dilutions in assay buffer keeping final DMSO ≤1% v/v; include vehicle controls and, if available, a positive control compound.
• Cell-based assays: Confirm cytotoxicity window; pre-warm media; add compound to achieve target concentration with gentle mixing; monitor precipitation.
• Analytical QC: Verify concentration by UV/Vis if chromophore present or by quantitative NMR/LC–MS with internal standard.

Replace with item-specific parameters once the structure and intended use are confirmed.

Biological Roles

Item-specific facts (from Product Data)

• No biological function or target information is provided. Research Use Only.

General notes

• Many life-science reagents labeled with short codes (e.g., “H7”) are small-molecule modulators or biochemicals. However, without a disclosed structure or target, no specific biological pathway, enzyme class, or binding mode can be credibly stated for this item.
• If your work involves biochemical assays or cellular studies, consult primary literature by CAS (2803681-14-1) and obtain target-annotation data (e.g., enzyme class, Ki/IC50 if applicable) before experimental design.
• For in vitro applications, document vehicle controls, final solvent fractions, and exposure times. For cell-based work, confirm cytotoxicity profiles independently under your assay conditions.
• No clinical or diagnostic use is intended or implied.

Buffer Applications

Relevance

• Buffer formulation details for this specific item are not provided. If H7 is an analyte or tool compound rather than a buffering agent, dedicated buffer guidance is typically not applicable.

General guidance (if preparing assay buffers for small-molecule tools)

• Choose buffers that maintain target protein stability and compound solubility (commonly PBS, HEPES, Tris; pH 6.8–8.0) and keep final organic co-solvent low (≤0.1–1% v/v DMSO or EtOH).
• Filter (0.22 µm) and degas buffers if using sensitive detection (e.g., SPR, ITC, HPLC-UV).
• Validate compound stability across pH and ionic strength; monitor for precipitation upon dilution from concentrated stocks.

Item-specific buffer capacity/pKa: Not applicable/not specified for this item; refer to CoA/Spec Sheet if later provided.

Green Alternatives

Context

• Item-specific green metrics (e.g., PMI, solvent class, hazard category) are not available for this product. The discussion below is general and should be aligned with the compound’s actual structure and use once known.

General considerations

• If H7 is used as a solvent or auxiliary: Prefer reagents aligned with solvent selection guides (CHEM21/ACS GCI). For example, replace chlorinated solvents with ethyl acetate, 2-MeTHF, CPME, or propylene carbonate where chemistry permits.
• If H7 is a small-molecule tool compound: “Greener” focus shifts to formulation and waste minimization—use high-concentration DMSO stocks to reduce solvent volume, adopt microscale assays, and select lower-toxicity co-solvents (EtOH, MeOH, MeCN) over DMF/NMP when feasible.
• Packaging and cold-chain: Consolidate orders to reduce shipments; retain cold packs for reuse; minimize dry ice where −20 °C stability is adequate.

Trade-offs

• Lower-toxicity solvents may reduce solubility or reaction rates; perform small trials to balance performance with environmental impact.

Pharmaceutical Uses

Item-specific facts (from Product Data)

• No pharmacopeial status, excipient role, or formulation guidance is provided for this product. Research Use Only; not for human or veterinary use.

General development considerations (non–item-specific)

• If used in pre-formulation research, typical vehicles for small molecules include DMSO concentrates diluted into PEG400/saline or cyclodextrin solutions for exploratory in vitro work. Confirm compound stability and avoid leachable interactions with plastics.
• For analytical support, establish an HPLC/UPLC assay with orthogonal detection (UV/FLD/MS) and a validated reference standard when available.
• No claims are made regarding API status, GMP suitability, or regulatory filings for this item.

Physical Properties

Item-specific facts (from Product Data)

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Physical state (solid/liquid), melting point, boiling point, density, refractive index, logP, pKa, and solubility: Not specified for this item; refer to CoA/Spec Sheet.

General/literature guidance (non–item-specific)

• If H7 is a small-molecule research reagent (common for life-science tools), typical lab practice is to assess solubility first in high-permittivity solvents (water, methanol, ethanol) and polar aprotics (DMSO, DMF, acetonitrile). Begin with a small-scale screening (1–5 mg) to identify a workable stock concentration.
• For hygroscopic or deliquescent materials, weigh rapidly in a low-humidity environment and store under desiccation; for volatile liquids, use gas-tight syringes or crimp vials.
• Record any observed color, crystal habit, and onset of melting (capillary) to aid future handling; compare with lot CoA once provided.

Quality & Grades

Item-specific facts (from Product Data)

• Grade/purity: Not specified for this item; refer to CoA/Spec Sheet.

How to interpret grades (general guidance)

• Research grade: Typical for discovery workflows; acceptance criteria focus on purity by HPLC/GC and ID by NMR/HRMS.
• Analytical/HPLC grade (for solvents/reagents): Low non-volatile residue and low UV background; suited for chromatography and spectroscopic assays.
• Bio/Cell culture grade: Additional controls on endotoxin/bioburden; only relevant if explicitly stated on the CoA.

Recommendations

• Request lot-specific purity, residual solvent profile, and water content where applicable. If using in bioassays, ask for endotoxin/bioburden data.
• Stabilizers/inhibitors (if present) impact reactivity and spectra; absence/presence must be confirmed on the CoA. No stabilizers are specified for this item in the catalog entry.

Reaction & Applications

Item-specific facts (from Product Data)

• Manufacturer applications: Not provided in the catalog entry.

General guidance

• Without structural information, specific reaction roles cannot be assigned. If H7 is intended as a biochemical tool compound, its primary “application” may be as a stock solution for in vitro/cellular assays rather than a synthetic reagent.
• If H7 is used in synthesis, consult the CoA and any available literature (searchable by CAS 2803681-14-1) to identify functional groups and assign reactivity prior to planning transformations.
• Practical tips irrespective of identity:
  • Verify identity and purity by orthogonal methods (e.g., HPLC-UV and 1H NMR) before committing to critical experiments.
  • Run small pilot reactions/assays to confirm performance and stability in the chosen medium.
  • Track stability at room temperature versus refrigerated/frozen conditions to set handling windows on the bench.

Reaction Conditions

Item-specific facts (from Product Data)

• No recommended reaction conditions are provided for this product.

General guidance framework (non–item-specific)

• Solvent: Select based on solubility and stability (DMSO, DMF, MeCN, toluene, dioxane, alcohols, aqueous buffers for bioconjugations).
• Temperature: Start at ambient; escalate cautiously while monitoring for decomposition. Record exotherms and color changes.
• Atmosphere: Use inert gas (N2/Ar) for air-/moisture-sensitive compounds; dry solvents and glassware when required.
• Stoichiometry and order of addition: Establish via small-scale DoE or factorial screens to reduce material consumption.
• Workup: Quench carefully, separate phases, and purify by chromatography or crystallization as compatible.

Note: Replace this generic guidance with reagent-specific conditions once the functional group profile is confirmed from the CoA or literature.

Safety & Handling

Item-specific facts (from Product Data)

• GHS classification, signal word, H-statements, pictograms: Not specified for this item; refer to SDS.
• Storage conditions: Store at −20 °C.
• Shipping: Ice chest + ice pads.

General safety guidance (non–item-specific; defer to SDS)

• Use standard PPE: lab coat, safety glasses, and appropriate chemically resistant gloves. Handle powders in a fume hood to minimize inhalation and dust exposure; handle liquids with secondary containment.
• Avoid incompatible conditions until the structure is confirmed; conservatively keep away from strong oxidizers, strong acids/bases, ignition sources, and reactive metals.
• First aid (overview, not a substitute for SDS): If on skin/eyes, rinse with water for ≥15 min; if inhaled, move to fresh air; if ingested, rinse mouth. Seek medical attention for any exposure or persistent symptoms.
• Waste: Collect in appropriate organic or aqueous hazardous waste per your institutional SOP.
• Note: If the reagent is moisture-/light-sensitive, maintain under inert atmosphere and protect from light as an added precaution alongside −20 °C storage.

Solvent Selection

Item-specific facts (from Product Data)

• No solvent recommendations or solubility data are provided for this item; refer to CoA/Spec Sheet.

General solvent strategy for small-molecule life-science reagents

• Primary screening: DMSO (high solvency, common for bioassay stocks), DMF, ethanol, methanol, water (if ionic/ionizable), and acetonitrile. Begin with 10–50 mg/mL attempts at room temperature, gentle warming (<40 °C), or sonication.
• Aqueous stocks: If water solubility is poor, prepare a DMSO concentrate (e.g., 10–100 mM) and dilute into buffered media maintaining final DMSO ≤0.1–1% v/v for cellular systems.
• pH adjustment: For ionizable compounds, modest pH shifts (pH 2–3 for bases; pH 9–10 for acids) can improve solubility; confirm compound stability at adjusted pH.
• Co-solvent blends: DMSO/water, ethanol/water, or MeCN/water can balance solvency and assay compatibility.
• Filtration: Use 0.22 µm PTFE for organics or PES/nylon for aqueous mixtures to remove particulates before dosing.
• Always document solvent system, concentration, and any visible precipitation upon dilution.

Storage & Reconstitution

Item-specific facts (from Product Data)

• Storage: Store at −20 °C.
• Shipping: Ice chest + ice pads.
• Reconstitution solvent, concentration limits, and stability after reconstitution: Not specified for this item; refer to CoA/Spec Sheet.

General best practices (non–item-specific)

• Upon receipt: Inspect packaging temperature; record lot, date, and conditions. Allow to equilibrate to room temperature in a desiccator before opening to avoid condensation.
• Aliquoting: If preparing solutions (e.g., in DMSO or aqueous buffer), make single-use aliquots in inert vials; minimize headspace and protect from light if chromophoric.
• Freeze–thaw: Avoid repeated cycles; store aliquots at −20 °C or −80 °C as appropriate. Thaw at room temperature and use promptly.
• Stability checks: Periodically assess purity by HPLC or LC–MS for long-term stored materials.
• Label all aliquots with concentration, solvent, date, and storage temperature. Research Use Only.

Structure & Identity

Item-specific facts (from Product Data)

• Product name: H7 (SKU: H1452440)
• CAS: 2803681-14-1
• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• InChIKey: Not specified for this item; refer to CoA/Spec Sheet.
• SMILES: Not specified for this item; refer to CoA/Spec Sheet.

General notes

• Structural features, functional groups, and stereochemistry cannot be described without a disclosed structure. If your application requires precise identity confirmation (e.g., NMR, HRMS, HPLC), request the latest Certificate of Analysis (CoA) and spectral pack.
• For internal documentation, align the CAS (2803681-14-1) with your LIMS entry and verify lot-matched identifiers on receipt (label, CoA, and packing list).

Synthetic Utility

Item-specific facts (from Product Data)

• No structural information is provided; specific synthetic roles cannot be assigned.

General strategy

• Before deploying an unfamiliar reagent, determine: functional groups, protecting-group compatibility, and redox sensitivity. Use small-scale test reactions to map reactivity.
• If H7 is a building block: Identify handles (e.g., halides, boronates, amines, acids) for cross-coupling (Suzuki–Miyaura, Buchwald–Hartwig), amide couplings (EDC/HATU), or nucleophile–electrophile elaborations.
• If H7 is a catalyst/ligand: Optimize loading, base/acid additive, and solvent polarity; confirm stability at operating temperature.
• Analytical control: Track conversions by LC–MS/TLC and confirm structure of intermediates by NMR/HRMS.

Because the identity is unspecified here, treat this section as a planning checklist rather than reagent-specific guidance.

Target Specificity

Item-specific facts (from Product Data)

• No target, antigen, enzyme, or receptor specificity data are provided for this item.

Guidance

• If H7 is a biochemical tool, obtain target annotations (e.g., UniProt IDs, enzyme family) and potency/selectivity metrics (IC50, Ki, Kd) from primary literature or vendor technical notes before use.
• Absent such data, design exploratory assays to independently determine activity and off-target profiles.