HA tag Mouse mAb (AF555) - Primary antibody, specific to HA

Cat. No.: Ab222052

GRADE & PURITY Validated, ExactAb™, High Performance, 0.5 mg/mL

Application

Flow Cytometry, IF/ICC

Host species

Mouse

Reactive species

Species independent

Isotype

Mouse IgG2b

Conjugation

AF555

Overview

Mouse anti HA tag Antibody, Monoclonal antibody(12CA5), could be used for Flow Cytometry, IF/ICC and so on.

Specifications

Product Name

HA tag Mouse mAb (AF555) - Primary antibody, specific to HA

Synonyms

HA epitope tag antibody | HA1 antibody | HA2 antibody | hemagglutinin antibody | Hemagglutinin HA1 chain antibody | Hemagglutinin HA2 chain antibody | HA Epitope Tag | HA Tag | HA | Hemagglutinin | HA tag antibody | Anti- HA tag antibody

Specifications & Purity

ExactAb™, Validated, High Performance, 0.5 mg/mL

Biochemical and Physiological Mechanisms

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression v

Host species

Mouse

Specificity

HA

Immunogen

Influenza virus hemagglutinin protein (AA 98-106).

Positive Control

Flow Cytometry: HEK293 cells transfected with HA and eGFP tags IF/ICC: HEK293 cells transfected with HA and eGFP tags

Conjugation

AF555

Excitation (nm)

Green Laser(532nm)

Emission(nm)

568nm

Ex/Em(nm)

Ex:553nm;Em:568nm

Grade

ExactAb™, High Performance, Validated

Purification Method

Protein G purified

Concentration

0.5 mg/mL

Storage and Shipping

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20°C. Protect from light. Avoid freeze/thaw cycles.

Images

IF/ICC: HA tag Mouse mAb (AF555) (Ab222052)

HA tag Mouse mAb (AF555) (Ab222052) - IF/ICC
IF analysis of HA tag (Red) in HEK293T cells transfected with a eGFP-HA tag. The cells were fixed with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 15 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with HA tag Mouse mAb (AF555) (Ab222052) at 1/1000 dilution in blocking buffer for 2 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Flow Cytometry: HA tag Mouse mAb (AF555) (Ab222052)

HA tag Mouse mAb (AF555) (Ab222052) - Flow Cytometry
Flow Cytometry analysis of HA tag expression by HEK293T cells transfected with a eGFP-HA tag (red) compared to an isotype control (blue). The cells were fixed with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 15 minutes and then stained with HA tag Mouse mAb (AF555) (Ab222052) at a dilution of 1/500 for 30 minutes at 4°C in the dark. Blue - Mouse IgG (AF555) (Ab190246).

Application

• Application: Dilution info
• IF/ICC:

  1/100-1/1000 ;

• Flow Cytometry:

  1:50-1:500;