🌎 Ship to: United States
Toggle Nav
Cart
Search
Same-day shipping on all in-stock items.
Advanced Search
Quick Order SDS / CoA Structure Bulk & Custom
Menu
Account
Settings
྾
224,000+ research products · Triple ISO certified · COA & SDS available for every product · Same-day shipping on in-stock items

IL-36α, Agonist of Interleukin-36 receptor, CAS No.rp174307, Agonist of Interleukin-36 receptor

CAS: rp174307 Cat. No.: rp174307
COA
IL-36α, Agonist of Interleukin-36 receptor, CAS No.rp174307, Agonist of Interleukin-36 receptor
AVAILABLE TO ORDER
GRADE & PURITY Moligand™ ? Moligand™ — Aladdin's line of ligands and bioactive small molecules. Use for receptor, pathway, and binding studies needing defined small-molecule tools.
★
Size
USA
Germany (EU)*
Price
Qty
500μg
rp174307-500μg
US Made to order · 2–4 wks ·
—
$2,399.90
1mg
rp174307-1mg
US Made to order · 2–4 wks ·
—
$3,999.90
Enter a quantity for the sizes you want to add.
Bulk / Custom
♡ Wish List ⇅ Compare ⎙ Print page
SDS
Safety data
COA
Certificate
DS
Datasheet
SP
Spec sheet
Specifications Storage and Shipping Documentation Advanced Data Associated Targets(Human) Certificates（CoA,COO,BSE/TSE and Analysis Chart) Genetic information Solution Calculators Reviews Application Protocols Biological Roles Buffer Applications Green Alternatives Pharmaceutical Uses Physical Properties Quality & Grades Reaction & Applications Reaction Conditions Safety & Handling Solvent Selection Storage & Reconstitution Structure & Identity Synthetic Utility Target Specificity
🧪
Why this grade

Moligand™ Moligand™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Room temperature Ships Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Specifications

Product Name
IL-36α, Agonist of Interleukin-36 receptor, CAS No.rp174307
Synonyms
FIL1 epsilon;interleukin-1 epsilon (IL-1 epsilon)
Grade
Moligand™
Specifications & Purity
Moligand™
Action Type
AGONIST
Mechanism of action
Agonist of Interleukin-36 receptor
CAS
rp174307
Molecule Type
Peptide
Product Line
biological_products
Storage and Shipping
Storage
Room temperature

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
IL1RL2 Tbio Interleukin-1 receptor-like 2 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
IL1RAP Tclin Interleukin-1 receptor accessory protein (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Certificates（CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Genetic information
Alternate NamesFIL1 epsilon;interleukin-1 epsilon (IL-1 epsilon)
Reference
  • 1. Kinetics and inhibition of recombinant human cystathionine gamma-lyase. Toward the rational control of transsulfuration., The Journal of biological chemistry, Steegborn, C C and 7 more authors.
  • 2. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences., Proceedings of the National Academy of Sciences of the United States of America, Strausberg, Robert L RL and 83 more authors.
  • 3. Genomic basis of cystathioninuria (MIM 219500) revealed by multiple mutations in cystathionine gamma-lyase (CTH)., Human genetics, Wang, Jian J and Hegele, Robert A RA.
  • 4. Cloning and nucleotide sequence of human liver cDNA encoding for cystathionine gamma-lyase., Biochemical and biophysical research communications, Lu, Y Y, O'Dowd, B F BF, Orrego, H H and Israel, Y Y.
  • 5. Single nucleotide polymorphism in CTH associated with variation in plasma homocysteine concentration., Clinical genetics, Wang, J J, Huff, A M AM, Spence, J D JD and Hegele, R A RA.
  • 6. Cystathionine gamma-lyase overexpression inhibits cell proliferation via a H2S-dependent modulation of ERK1/2 phosphorylation and p21Cip/WAK-1., The Journal of biological chemistry, Yang, Guangdong G, Cao, Kun K, Wu, Lingyun L and Wang, Rui R.
  • 7. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)., Genome research, Gerhard, Daniela S DS and 115 more authors.
  • 8. Towards a proteome-scale map of the human protein-protein interaction network., Nature, Rual, Jean-François JF and 37 more authors.
  • 9. The DNA sequence and biological annotation of human chromosome 1., Nature, Gregory, S G SG and 178 more authors.
  • 10. Polymorphisms in one-carbon metabolism and trans-sulfuration pathway genes and susceptibility to bladder cancer., International journal of cancer, Moore, Lee E LE and 14 more authors. more
Solution Calculators
Reviews

Customer Reviews

Application Protocols
  • Item-specific (Product Data)
    • Tested applications, recommended dilutions, positive controls: Not specified for this item; refer to CoA/Spec Sheet.
  • Example, literature-guided workflows (to be optimized by the user)
    • NF-κB reporter assay
      • Plate IL-36R-expressing cells at assay density; allow to adhere overnight.
      • Prepare a dilution series of IL-36α in assay medium containing 0.1% carrier protein.
      • Treat cells for 4–8 h; quantify reporter signal. Include vehicle and IL-36Ra controls.
    • qPCR cytokine induction
      • Stimulate keratinocytes or epithelial cells with a titration of IL-36α for 4–24 h.
      • Extract RNA, synthesize cDNA, and quantify target mRNAs (e.g., CXCL1/2) relative to housekeeping genes.
    • Phospho-signaling (Western blot)
      • Treat responsive cells for 5–60 min.
      • Lyse and probe for p-p38, p-JNK, p-ERK, or IκBα degradation to confirm pathway engagement.
    • Notes
      • Use low-bind plastics; prepare fresh dilutions; avoid multiple freeze–thaw cycles.
      • Optimize dosing, time course, and medium conditions for each cell model. These are literature-style examples, not validated protocols for this specific item.
Biological Roles
  • Literature/general overview (no clinical claims)
    • Family and identity: IL-36α (IL1F6) is a member of the IL-1 cytokine family. It signals via IL-36R (IL1RL2) in conjunction with IL-1RAcP to activate NF-κB and MAPK pathways in responsive cells, notably epithelial and certain immune cells.
    • Activation and regulation: The zymogen-like precursor is weakly active until N-terminal proteolysis by neutrophil-derived proteases (e.g., cathepsin G, elastase) generates higher-potency forms. Activity is antagonized by IL-36 receptor antagonist (IL-36Ra), providing pathway-level control (literature).
    • Cellular responses: Induces expression of chemokines (e.g., CXCL1/2/8), cytokines, and antimicrobial peptides in keratinocytes and barrier tissues, shaping leukocyte recruitment and barrier defense programs (literature).
    • Tissue context: Prominent roles at epithelial interfaces such as skin and airway mucosa; contributes to modeling epithelial–immune crosstalk in vitro (literature).
    • Research utility: Used as a defined agonist to study IL-1 family signaling specificity, receptor pharmacology, and downstream transcriptional networks. Appropriate for pathway mapping, high-content transcriptomics/proteomics, and screening of modulators in cell-based systems.
    • Note: Specific sequence, post-translational modifications, and bioactivity units for this catalog item are not provided here; consult the CoA/Spec Sheet for item-specific biological attributes.
Buffer Applications
  • Item-specific (Product Data)
    • No formulation or buffer recipe is provided for this item; refer to CoA/Spec Sheet.
  • Literature/general guidance for protein cytokines
    • Working buffers: Neutral pH buffers such as PBS (pH ~7.2–7.6) or 10–50 mM HEPES with 100–300 mM NaCl are commonly used for reconstitution and dilution.
    • Stabilizers: Adding 0.1% carrier protein (BSA/HSA) or 0.05–0.1% trehalose/sucrose can reduce surface adsorption and improve recovery in low-concentration applications.
    • Filtration: For sterile work, 0.22 µm filtration of buffers is recommended; avoid filtering highly dilute protein solutions to prevent losses.
    • Storage buffer considerations: Avoid chelators or detergents that might interfere with downstream assays unless validated. Include reducing agents only if specified for your protocol, as they may affect protein stability.
    • Aliquoting: Prepare single-use aliquots in low-bind tubes to maintain consistency across experiments and reduce degradation.
    • Note: Exact pH, ionic strength, and additives should be validated for your cell model and readout to avoid assay interference.
Green Alternatives
  • Not typically applicable
    • IL-36α is a protein cytokine used in biological assays rather than a process solvent or reagent. Concepts of green solvent substitution or reagent atom economy do not directly apply.
  • Practical sustainability notes (general)
    • Minimize waste by preparing small aliquots to avoid repeated freeze–thaw and discard.
    • Use recyclable low-bind plastics where validated, and plan experiments to reduce unused reconstituted protein.
    • For cleaning, prefer mild, biodegradable detergents; avoid harsh oxidizers that denature proteins and complicate waste handling.
Pharmaceutical Uses
  • Not a clinical/therapeutic product
    • This material is designated For research use only and is not intended for human or veterinary use.
  • Literature/general formulation context
    • Role in development workflows: Recombinant cytokines like IL-36α are used as research reagents to develop, optimize, and benchmark bioassays (e.g., receptor activation, reporter assays) supporting discovery programs.
    • Reference standard potential: May serve as an in-lab reference agonist for IL-36R pathway assays during screening of candidate modulators, subject to in-house qualification.
    • Pharmacopeia/compendial status: No pharmacopeial monograph is implied for this item. Specifications such as potency units, residuals, and endotoxin content, if needed for regulated contexts, must be established by the user or obtained from the CoA/Spec Sheet.
    • Manufacturing considerations: For any translational work, higher-grade, GMP-compliant materials are typically required; this Moligand™ item is not specified as GMP-grade.
Physical Properties
  • Item-specific (Product Data)
    • Appearance: Not specified for this item; refer to CoA/Spec Sheet.
    • Molecular Weight: Not specified for this item; refer to CoA/Spec Sheet.
    • Physical constants (bp, mp, density, refractive index, UV cutoff, water/peroxide/metal content): Not specified for this item; refer to CoA/Spec Sheet.
  • Literature/general for protein cytokines like IL-36α
    • Aggregation state: Typically monomeric in dilute buffered aqueous solution; may self-associate at higher concentrations depending on buffer and ionic strength (literature, protein-general).
    • Solubility: Soluble in physiological buffers (e.g., PBS, HEPES) with added carrier protein (0.1% BSA or HSA) or inert polymer (0.05–0.1% trehalose) to reduce adsorption; salt and pH modulate solubility (literature). Avoid strong organic solvents.
    • Stability: Sensitive to repeated freeze–thaw and surface adsorption; stability enhanced by low protein concentration handling in siliconized/low-bind plastics and by inclusion of stabilizers (literature).
    • Isoelectric and charge: Exact pI depends on sequence/construct; IL-36 family members often display moderate basicity; confirm for this item via CoA/Spec Sheet or sequence calculation (literature note).
    • Spectroscopy: Proteins exhibit UV absorbance at 280 nm due to aromatic residues; extinction coefficient is sequence-dependent and not specified for this item (literature).
    • Lyophilizates: If supplied lyophilized, moisture uptake can change mass and reconstitution volume; consult the specific label (general guidance).
Quality & Grades
  • Item-specific (Product Data)
    • Grade/Purity: Moligand™.
  • Interpretation and context (general)
    • Moligand™ (general description): Denotes screening-grade materials suitable for ligand discovery, assay development, and library screening. It emphasizes research-use suitability rather than clinical or GMP compliance. Exact assay specifications (purity %, endotoxin levels, bioactivity units, carrier content) are not specified for this item; refer to CoA/Spec Sheet.
    • What to verify before use:
      • Identity/format: Confirm amino-acid sequence, expression host, and any tags/carriers if relevant to your assay.
      • Activity/readout: Check bioactivity validation method (e.g., IL-36R reporter activation) and unit definition if provided in the CoA.
      • Purity profile: Review SDS-PAGE/HPLC data, residual host-cell proteins/DNA, and aggregation content if your application is sensitive.
      • Endotoxin: For cell-based assays with sensitive lines, verify endotoxin specification (EU/mg) on the CoA.
    • Suitability by application:
      • High-throughput screening and pathway mapping: Typically appropriate.
      • Quantitative potency benchmarking or in vivo work: Additional qualification may be needed; this catalog item is for research use only.
Reaction & Applications
  • Applicability to chemical reactions
    • IL-36α is a biological ligand/cytokine, not a synthetic reagent. It is not typically used in organic reaction chemistry. See Biological Roles, Application Protocols, and Reaction Conditions (bioassay) for relevant usage.
  • Research applications (literature/general)
    • Pathway interrogation: Activates IL-36R/IL-1RAcP signaling, enabling studies of NF-κB and MAPK cascades in epithelial and myeloid models.
    • Cell biology: Stimulates keratinocytes, dendritic cells, and other responders to model inflammatory gene programs in vitro.
    • Screening: Serves as an agonist ligand in unbiased or targeted small-molecule/antibody screens for IL-36 axis modulation.
    • Protease processing studies: Substrate for neutrophil-derived proteases (e.g., cathepsin G, elastase) to examine activation by N-terminal trimming (literature).
    • Crosstalk: Useful in combination with other IL-1 family cytokines (e.g., IL-1β, IL-36Ra) to map antagonism/synergy in signaling assays (literature).
Reaction Conditions
  • Not chemical reaction conditions; bioassay context (literature/general guidance)
    • Cell-based stimulation: Cytokine agonists like IL-36α are commonly used in the approximate range of 0.1–100 ng/mL, with exposure times from 2–24 h depending on endpoint (e.g., NF-κB reporter, qPCR of target genes). Optimize empirically for your cell model. These are literature-reported ranges, not item specifications.
    • Buffer and carrier: Use serum-free or defined medium if needed for clean readouts; include 0.1% BSA/HSA or 0.05–0.1% trehalose to minimize adsorption losses when preparing serial dilutions.
    • Temperature/atmosphere: Standard mammalian cell culture conditions (37°C, 5% CO2) for cell-based assays; for biochemical receptor-binding assays, 4–25°C depending on kinetics.
    • Controls: Include vehicle controls, receptor antagonist (e.g., IL-36Ra) where appropriate, and pathway inhibitors to confirm specificity (literature).
    • Data quality tips: Pre-block plasticware with carrier-containing buffer; prepare fresh dilutions; avoid repeated freeze–thaw; confirm dose–response linearity and EC50 within expected ranges for your cell system.
    • Note: No potency units or specific activity are provided for this item—consult the CoA/Spec Sheet for any available activity verification.
Safety & Handling
  • Item-specific (Product Data)
    • Storage Conditions: Room temperature.
    • Signal Word, H-Statements, GHS Classification, Pictograms: Not specified for this item; refer to SDS.
  • General laboratory safety for recombinant proteins/cytokines (literature/practice)
    • PPE: Wear lab coat, nitrile gloves, and safety glasses. Handle powders/lyophilizates in a clean area to minimize aerosolization.
    • Incompatibilities: Avoid strong oxidants, proteases, and extreme pH that may denature proteins. Minimize exposure to elevated temperatures and UV.
    • Engineering controls: Work in a clean environment; for bioassays with human/animal cells use appropriate biosafety practices.
    • First aid (overview; defer to SDS): If inhaled as dust, move to fresh air; if on skin/eyes, rinse with water; if ingested, rinse mouth. Seek medical advice if symptoms persist.
    • Special risks: Proteins can cause sensitization after prolonged exposure in some individuals. Avoid repeated skin contact or inhalation of dusts/aerosols.
    • Waste: Dispose according to institutional guidelines for non-hazardous biochemical waste unless SDS specifies otherwise.
    • Always defer to the product-specific SDS and institutional EHS guidance for authoritative hazard and response information.
Solvent Selection
  • Item-specific (Product Data)
    • No solvent/reconstitution details are specified; refer to CoA/Spec Sheet.
  • Literature/general guidance for protein cytokines
    • Primary solvent: Aqueous buffers (e.g., PBS, 10–50 mM HEPES, pH ~7.2–7.6). Avoid organic solvents that denature proteins.
    • Additives to minimize adsorption: 0.1% carrier protein (BSA/HSA) or 0.05–0.1% nonionic polymers (trehalose, sucrose) can reduce loss to vessel walls (literature).
    • Ionic strength: 100–300 mM NaCl often improves solubility and stability by screening electrostatic interactions (literature).
    • pH considerations: Maintain near-neutral pH to preserve IL-1 family tertiary structure; avoid strong acid/base.
    • Container selection: Use low-bind plastics or siliconized glass; pre-rinse with buffer containing carrier to minimize nonspecific binding.
    • When to choose alternatives: If assay demands serum-free conditions, validate stabilizers that do not interfere with readouts (e.g., use trehalose instead of BSA in some fluorescence assays).
Storage & Reconstitution
  • Item-specific (Product Data)
    • Storage Conditions: Room temperature.
    • Shipped In: Not specified for this item; refer to CoA/Spec Sheet.
    • Reconstitution instructions: Not specified for this item; refer to CoA/Spec Sheet.
  • General guidance (literature/practice; verify against the item’s label)
    • If lyophilized: Briefly centrifuge vial to collect material. Reconstitute with sterile buffer (e.g., PBS or HEPES, pH ~7.2–7.6) optionally containing 0.1% carrier protein or 0.05–0.1% trehalose to minimize adsorption.
    • Aliquoting: Prepare single-use aliquots in low-bind tubes to avoid repeated handling.
    • Short-term handling: Keep reconstituted solutions on ice during use; avoid vigorous vortexing/foaming.
    • Freeze–thaw: Minimize cycles; if freezing, snap-freeze aliquots and store per your internal practice once compatibility with room-temperature storage is clarified by the CoA/label.
    • Container: Use low-bind plastics or siliconized glass. Pre-wet with buffer containing carrier to reduce loss.
    • Always defer to the product label and CoA/Spec Sheet for definitive instructions for this specific item.
Structure & Identity
  • Item-specific (Product Data)
    • Product Name: IL-36α (SKU: rp174307)
    • Grade/Purity: Moligand™
    • CAS: rp174307 (catalog placeholder, not a registry CAS)
    • Molecular Formula: Not specified for this item; refer to CoA/Spec Sheet.
    • Molecular Weight: Not specified for this item; refer to CoA/Spec Sheet.
    • InChIKey: Not applicable/not provided for proteinaceous analytes; refer to CoA/Spec Sheet.
    • SMILES: Not applicable/not provided for proteinaceous analytes; refer to CoA/Spec Sheet.
  • Literature/general (for IL-36α proteins)
    • Identity: IL-36α (also known as IL1F6) is a cytokine of the IL-1 family.
    • Structural features: Single-chain polypeptide that adopts the β-trefoil fold characteristic of IL-1 family cytokines; lacks disulfide bonds typical of some secreted proteins; activated by N-terminal proteolytic processing.
    • Receptor interactions: Binds IL-36 receptor (IL-36R/IL1RL2) in complex with IL-1 receptor accessory protein (IL-1RAcP).
    • Sequence length: Human IL-36α is typically ~158 aa (full-length precursor); active forms are shorter N-terminally processed species (literature). No specific sequence for this catalog item is provided here.
    • 2D description in words: A compact, globular cytokine domain with three pseudo-symmetric β-hairpin trefoils forming a 12-stranded β-barrel-like architecture; N-terminus influences activity after proteolysis (literature).
Synthetic Utility
  • Not applicable to organic synthesis
    • IL-36α is a protein ligand and does not function as a building block, catalyst, or reagent in chemical synthesis. It is instead employed as a biological agonist in cell-based or biochemical assays.
  • Related experimental utility (literature/general)
    • Positive control ligand: Useful to validate IL-36R expression/function in engineered cell lines or primary cells.
    • Pathway selectivity studies: Enables dissection of IL-1 family receptor signaling specificity versus IL-1β, IL-33, etc.
    • Protease processing assays: Serves as a substrate to study activation by N-terminal trimming enzymes.
Target Specificity
  • Item-specific (Product Data)
    • Antigen/target, species reactivity, clone/isotype: Not specified for this item; refer to CoA/Spec Sheet.
  • Literature/general for IL-36α
    • Primary receptor: IL-36 receptor (IL1RL2) with IL-1RAcP as accessory protein (heterodimeric signaling complex).
    • Downstream pathways: Canonical activation of NF-κB and MAPK cascades in IL-36R-expressing cells (literature).

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Moligand™ grade guide →
Bulk & Custom Packaging Need larger quantities or custom specifications? Contact our team for a tailored quote.

View related series

Interleukin-36 receptor Agonist (3)
We use cookies to ensure the website functions properly and, where permitted, to improve your experience. You can manage your preferences at any time in Settings. Learn more in our Cookie Policy.

Shall we send you a message when we have discounts available?

Remind me later

Thank you! Please check your email inbox to confirm.

Oops! Notifications are disabled.

Privacy Policy Terms of Use Cookie Policy Terms and Conditions of Sale Sales Policy
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
Copyright © 2023–present Aladdin Scientific Corp. All rights reserved.