Recombinant Human INAVA Protein: Properties & Research Uses

Recombinant Human INAVA Protein is a research-grade recombinant protein that plays a pivotal role in innate immune signaling and has emerged as an important tool for studying inflammatory diseases such as inflammatory bowel disease.

Protein Name

Recombinant Human INAVA Protein

Also Known As

INAVA; Innate Immunity Activator; C1orf141

Species

Human (Homo sapiens)

Product SKU

rp370885

Research Area

Innate Immunity, Inflammatory Disease, Signal Transduction

Format

Recombinant protein (lyophilized or liquid; verify with datasheet)

What Is INAVA? Biological Background

INAVA (Innate Immunity Activator), also referred to by the gene alias C1orf141, is a protein encoded in the human genome that functions as a positive regulator of innate immune signaling. It was identified through genetic studies as a susceptibility gene associated with inflammatory bowel disease (IBD), linking it to the pathogenesis of Crohn's disease and ulcerative colitis.

INAVA is expressed in various immune cell types, including monocytes and macrophages, and participates in pattern recognition receptor signaling pathways. It has been reported to interact with components of innate immune cascades, facilitating downstream inflammatory responses upon microbial challenge. Its involvement in both the NF-κB and other inflammatory pathways makes it a protein of significant interest for researchers investigating mucosal immunity, gut inflammation, and autoimmune conditions.

Molecular Properties of Recombinant Human INAVA Protein

Recombinant Human INAVA Protein is produced using recombinant DNA technology to ensure consistent quality and batch-to-batch reproducibility. The recombinant form of INAVA retains the key structural and functional attributes of the native protein, making it suitable for a wide range of in vitro assays.

Key molecular characteristics include its defined amino acid sequence derived from the human INAVA gene, appropriate folding to support protein–protein interaction studies, and production under controlled bioprocessing conditions. Researchers should consult the product datasheet (SKU: rp370885) for the confirmed molecular weight, purity grade, buffer composition, and storage conditions, as these parameters are critical for experimental reproducibility.

Purity is typically assessed by SDS-PAGE and/or HPLC, and endotoxin levels are controlled to minimize non-specific immune activation in cell-based assays. Specific activity and formulation details are provided in the accompanying certificate of analysis.

Research Applications

Recombinant Human INAVA Protein is a versatile research tool with applications across multiple experimental formats:

• Protein–Protein Interaction Studies: INAVA's role as a scaffold or adaptor in innate immune complexes makes it suitable for co-immunoprecipitation, pull-down assays, and surface plasmon resonance (SPR) experiments to map its interactome.
• Functional Assays: Researchers can use recombinant INAVA to reconstitute signaling cascades in cell-free systems or supplement cell-based models to study its effect on NF-κB activation and cytokine production.
• Antibody Development and Validation: Recombinant INAVA protein serves as an antigen for generating and validating anti-INAVA antibodies for use in ELISA, western blot, and immunofluorescence applications.
• IBD and Autoimmunity Research: Given its genetic association with IBD, recombinant INAVA is used in mechanistic studies exploring how INAVA variants contribute to disease susceptibility and immune dysregulation.
• Drug Target Research: As a node in innate immune signaling, INAVA represents a potential target for small-molecule or biologic intervention in chronic inflammatory conditions.

Handling, Storage, and Quality Considerations

Proper handling of Recombinant Human INAVA Protein is essential to maintain its biological activity and ensure reliable experimental results. The following general guidelines apply, though researchers should always defer to the product-specific datasheet for authoritative instructions:

• Storage: Recombinant proteins are typically stored at −20°C or −80°C. Avoid repeated freeze–thaw cycles, which can cause protein aggregation and loss of activity. Aliquot the protein upon first thaw if multiple uses are anticipated.
• Reconstitution: If supplied in lyophilized form, reconstitute in the recommended buffer (e.g., sterile PBS or the supplier-specified diluent) and allow the solution to equilibrate before use.
• Purity and Endotoxin: Confirm purity (≥85% or as stated on the CoA) and endotoxin levels prior to use in cell-based assays to avoid confounding results from LPS contamination.
• Working Concentration: Optimal concentrations for specific assay formats should be determined empirically. Titration experiments are recommended to establish dose–response relationships.

For any quality-related inquiries or technical support regarding SKU rp370885, contact Aladdin Scientific's technical support team.

Frequently asked questions

What is INAVA protein and why is it relevant to inflammatory disease research?

INAVA (Innate Immunity Activator, gene alias C1orf141) is a human protein that functions as a positive regulator of innate immune signaling. It was identified as a susceptibility gene for inflammatory bowel disease (IBD), including Crohn's disease and ulcerative colitis, making it highly relevant for researchers studying mucosal immunity and gut inflammation.

What assay formats are compatible with Recombinant Human INAVA Protein?

Recombinant Human INAVA Protein is compatible with a variety of assay formats, including protein–protein interaction studies (e.g., pull-down, co-immunoprecipitation, SPR), functional signaling assays, antibody generation and validation (ELISA, western blot, immunofluorescence), and mechanistic studies of innate immune pathways. Researchers should optimize working concentrations empirically for each application.

How should Recombinant Human INAVA Protein be stored to maintain activity?

Recombinant Human INAVA Protein should generally be stored at −20°C or −80°C and protected from repeated freeze–thaw cycles. Upon first thaw, it is advisable to aliquot the protein into single-use volumes. Always refer to the product datasheet for SKU rp370885 for specific storage buffer, temperature, and shelf-life recommendations.

What purity level can be expected for Recombinant Human INAVA Protein from Aladdin Scientific?

Purity specifications are provided in the certificate of analysis (CoA) accompanying each lot of SKU rp370885. Recombinant proteins of this class are commonly assessed by SDS-PAGE and/or HPLC. Researchers should review the CoA for lot-specific purity data and endotoxin levels prior to use in sensitive cell-based assays.

References

1. Yan J et al. INAVA has been identified through genome-wide association studies as a susceptibility locus for inflammatory bowel disease. (General reference — specific citation requires verification.)
2. Aladdin Scientific. Recombinant Human INAVA Protein, SKU rp370885. Product datasheet. (Supplier datasheet — consult current version for authoritative specifications.)
3. absea. PP-12680. Referenced in product description as source material. (Internal reference — verify cross-listing details.)

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.