Can DAPI be excited with laser confocal λ=405nm after staining the nucleus?
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Can DAPI be excited with laser confocal λ=405nm after staining the nucleus?
·Reviewed·Published ·Updated ·8 min read
Can DAPI be excited with laser confocal λ=405nm after staining the nucleus?
Yes, DAPI can be excited using 405 nm excitation light. Although the best wavelength for excitation of DAPI is in the ultraviolet region (~350-360 nm), it can also be effectively excited with blue light at 405 nm. Using a 405 nm excitation light source can still activate DAPI and produce blue fluorescence for viewing and imaging cell nuclei.
Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.
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Aladdin Scientific. "Can DAPI be excited with laser confocal λ=405nm after staining the nucleus?" Aladdin Knowledge Base, updated Aug 27, 2024. https://www.aladdinsci.com/us_en/faqs/can-dapi-be-excited-with-laser-confocal-after-staining-en.html
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