L343 , CAS No.1443520-22-6

CAS: 1443520-22-6 Cat. No.: L1439967 Formula: C₅₁H₉₅NO₆ Molecular Weight: 818.30

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Overview

L343 is an ionizable cationic lipidoid and can be used to synthetic liposomes for systemic delivery of RNAi therapeutics.

Specifications

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Names and Identifiers

Molecular Weight

818.30

Application Protocols

No tested application protocols are provided for this product.

• Item-specific assay conditions, concentrations, or controls: Not specified for this item; refer to CoA/Spec Sheet or library user guide.

General guidance (non-item-specific)

• Stock preparation: Prepare a concentrated stock (e.g., 10–50 mM) in DMSO, then dilute into assay medium to desired final concentration while keeping DMSO ≤0.1–1% v/v, as assay permits.
• Controls: Include vehicle controls, positive reference compounds (if known for the assay), and evaluate potential interference (autofluorescence, absorbance) at relevant wavelengths.
• Stability: Minimize freeze–thaw by aliquoting; confirm stability by LC–MS after storage if used longitudinally in screening campaigns.

Biological Roles

Item-specific facts

• Biological target/role: Not specified for this item; refer to literature or CoA if provided.

General context

• As a member of a small-molecule/compound library, L343 may be used as a test article in biological assays. Without structural or mechanistic information, no claims can be made regarding endogenous roles, pathways, or target engagement.

Recommended practices (general)

• Determine cytotoxicity windows and nonspecific assay interference (e.g., PAINS/IMAP, redox cycling, fluorescence interference) once the structure is available.
• Characterize stability in assay media (chemical and metabolic) and assess protein binding if relevant to the model system.
• Use orthogonal assays to confirm observed activities and reduce false positives due to aggregation or assay artifacts.

Note: All uses are for research purposes only; no clinical or diagnostic applications.

Buffer Applications

This product is not presented as a buffering agent, and no acid/base properties are disclosed.

• Item-specific buffer data: Not specified for this item; refer to CoA/Spec Sheet.

If L343 is to be tested in buffered systems, select a buffer based on assay needs (e.g., phosphate, HEPES, Tris) and the compound’s pKa once known. Maintain a low percentage of organic cosolvent (commonly ≤0.1–1% DMSO) to avoid perturbing buffer capacity or biological readouts.

Green Alternatives

Without structural and use-context details for L343, specific greener substitutions cannot be prescribed.

General guidance (green chemistry principles, literature)

• Solvent selection: Prefer safer solvents for handling and waste minimization during dissolution, purification, and analysis. When feasible, choose ethanol, isopropanol, 2-MeTHF, CPME, or water over higher-toxicity solvents; balance this with solubility and assay constraints.
• Process minimization: Use microplate-scale operations and high-concentration stocks to reduce solvent volume and waste.
• Energy and stability: Store at −20°C as specified, but minimize freeze–thaw cycles by aliquoting to reduce energy usage and material loss.

Example comparison (general; not item-specific)

• DMSO vs PEG-400/water cosolvents: DMSO is widely used but consider PEG-400 or aqueous cosolvent systems for certain bioassays if compatible, noting differences in viscosity and compound carry-over.

Ultimately, the most impactful green choice is often optimizing concentration and assay design to reduce overall solvent and consumable use, independent of the compound identity.

Pharmaceutical Uses

No excipient/formulation roles or pharmacopeial status are provided for L343.

• Item-specific regulatory status: Not specified for this item; refer to CoA/Spec Sheet.
• Research Use Note: For research use only.

General note: Library compounds are commonly employed in discovery-phase research (screening, SAR, analytical method development) and are not intended for human or veterinary use, API manufacture, or clinical diagnostics. If you require a material for regulated applications, obtain an appropriately qualified grade and full regulatory documentation.

Physical Properties

Item-specific facts (from Product Data)

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Melting point (MP): Not specified for this item; refer to CoA/Spec Sheet.
• Boiling point (BP): Not specified for this item; refer to CoA/Spec Sheet.
• Density: Not specified for this item; refer to CoA/Spec Sheet.
• Solubility: Not specified for this item; refer to CoA/Spec Sheet.
• LogP, pKa, refractive index: Not specified for this item; refer to CoA/Spec Sheet.

General guidance for small-molecule library compounds (literature/general)

• Typical formats: provided as dry film or as DMSO stock (e.g., 10 mM). If supplied dry, reconstitute in a suitable solvent (DMSO is commonly used for screening libraries) and then dilute into assay buffers with appropriate cosolvent limits (often ≤0.1–1% DMSO final).
• Solubility screening: Begin with DMSO; if inadequate, consider DMF, ethanol, or co-solvent systems (DMSO:water or ethanol:buffer). Verify clarity and absence of precipitate by visual inspection and, if important, nephelometry or HPLC.
• Hygroscopicity/polymorphism: Library compounds may be hygroscopic or exhibit multiple solid forms; confirm mass post-drying and, if needed, perform Karl Fischer or TGA to assess residual moisture (general best practice; not item-specific).

Always defer to the item’s CoA/SDS for authoritative property data.

Quality & Grades

Item-specific facts (from Product Data)

• Grade/Purity: Not specified for this item; refer to CoA/Spec Sheet.

Context and expectations (general guidance)

• Small-molecule library members are typically supplied at research grade, accompanied by analytical verification such as LC–MS purity (e.g., area %), and sometimes 1H NMR confirmation. In the absence of explicit grade/purity in the catalog entry, consult the CoA for the batch-specific data, including chromatographic method, detection wavelength(s), and any observed impurities.
• If optical activity is relevant, a chiral assay (e.g., chiral HPLC) may be listed on the CoA. Absent such data, assume racemic unless specified.
• Stabilizers: None are listed for this item. If a stabilizer or salt form is used to enhance stability/solubility, it will appear on the CoA/specification.

Quality control suggestions (general)

• Verify identity and purity upon receipt when project-critical (LC–MS, HPLC purity, and, if necessary, NMR). Record storage history and any reformatting to maintain chain-of-custody.

Reaction & Applications

Item-specific facts

• Manufacturer application notes: Not provided.

Given the lack of structural information, L343 is not described here as a synthetic reagent. It is listed within a small-molecule/compound library category and is most likely intended for screening or profiling rather than as a stoichiometric or catalytic reagent in preparative chemistry.

General applications for library compounds (non-item-specific)

• Primary screening and SAR: Use as a test article in biochemical, biophysical, or cell-based assays to identify activity patterns.
• Profiling: Off-target liability panels, physicochemical profiling (logD, solubility, stability), and permeability assessments.
• Analytical reference: If structure is known, may serve as a reference standard for LC–MS method development.

If you intend to use L343 in synthetic chemistry, first obtain the full structure and assess functional group compatibility; then consult the Synthetic Utility and Reaction Conditions sections accordingly. Without structural data, no specific reaction roles can be responsibly recommended.

Reaction Conditions

No reaction conditions can be recommended for L343 without structural and functional group information.

• Item-specific catalytic/stoichiometric roles: Not specified for this item; refer to CoA/Spec Sheet.

General considerations (literature/general)

• For any planned transformations on a known structure, define moisture/air sensitivity, thermal stability, and solvent compatibility by small-scale trials and analytical monitoring (TLC/HPLC/LC–MS).
• Typical conditions for library scaffold modifications often include Pd-catalyzed cross-couplings, amide couplings (carbodiimide/HATU), and heteroatom alkylations under mild bases—selected strictly on the basis of the known functional groups.

Safety & Handling

Item-specific facts (from Product Data)

• GHS classification, signal word, pictograms, H-statements: Not specified for this item; refer to SDS.
• Storage conditions: Store at −20°C.
• Shipping: Ice chest + ice pads.

General safety guidance for small organic research compounds (not item-specific)

• PPE: Lab coat, safety glasses, and suitable chemical-resistant gloves (e.g., nitrile). Work in a fume hood to avoid inhalation of dust or solvent vapors.
• Avoid contact with skin, eyes, and clothing. Prevent ingestion and inhalation. Wash thoroughly after handling.
• Incompatibilities: Until structure is known, avoid strong oxidizers/reducers, strong acids/bases, and sources of ignition. Do not mix with unknown chemicals.
• First aid (overview): If on skin/eyes, rinse with water for ≥15 min and seek medical attention. If inhaled, move to fresh air. If ingested, do not induce vomiting; seek medical attention immediately.
• Spill/cleanup: Contain solid with inert absorbent; collect into compatible waste. Ventilate area. Dispose according to institutional and local regulations.

Always consult the product’s SDS for definitive hazard, toxicological, and disposal information.

Solvent Selection

Because no solubility or structural information is provided for L343, solvent choice should follow a pragmatic screening workflow rather than a fixed recommendation.

Item-specific facts

• Solubility: Not specified for this item; refer to CoA/Spec Sheet.

General solvent screening strategy (literature/general)

• Primary solvent: DMSO is the default for many discovery libraries due to broad solvency and assay compatibility at low percentages.
• Secondary options: DMF, ethanol, methanol, acetonitrile. For water-compatible working solutions, prepare a concentrated organic stock (e.g., 10–50 mM) and dilute into aqueous buffers, keeping v/v organic to the assay’s tolerance.
• If hydrophobic: Consider co-solvent systems (e.g., 20–50% acetonitrile or ethanol in buffer) or inclusion of non-ionic surfactants at assay-permitted levels.
• If acidic/basic functional groups are later identified: Use pH adjustment to increase solubility (e.g., form salts in situ), while confirming assay robustness to pH and counter-ions.

Small comparison (general)

• DMSO vs DMF: DMSO has better biocompatibility in many assays; DMF can offer improved solvency but is less favored biologically.
• Ethanol vs acetonitrile: Ethanol is more benign for cells/enzymes at low %; acetonitrile offers lower UV cutoffs for analytical methods.

Validate final solvent choice by visual clarity and analytical confirmation (HPLC/UPLC) of non-precipitating behavior at working concentrations.

Storage & Reconstitution

Item-specific facts (from Product Data)

• Storage conditions: Store at −20°C.
• Shipping: Ice chest + ice pads.

General handling guidance (not item-specific)

• Format: If supplied as a solid, allow container to equilibrate to room temperature in a desiccator before opening to avoid condensation. If supplied as a DMSO solution, keep tightly capped and minimize headspace to reduce moisture uptake.
• Reconstitution: In the absence of item-specific instructions, dissolve in an appropriate solvent—DMSO is commonly used for small-molecule libraries—then, if required, dilute into aqueous buffer immediately before use. Filter through a 0.22 µm PTFE syringe filter if particulate is observed and filtration is compatible with the compound.
• Aliquoting: Prepare single-use aliquots to avoid repeated freeze–thaw cycles. Record concentration and date of preparation on each aliquot.
• Stability checks: Periodically verify concentration and purity by LC–MS/HPLC when used over extended periods.

For definitive storage limits, solution stability, and reconstitution solvents specific to L343, consult the product’s CoA and SDS.

Structure & Identity

Item-specific facts (from Product Data)

• SKU: L1439967
• Product name: L343
• CAS: 1443520-22-6
• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• SMILES: Not specified for this item; refer to CoA/Spec Sheet.
• InChIKey: Not specified for this item; refer to CoA/Spec Sheet.

Interpretive notes

• The CAS Registry Number uniquely identifies the substance, but no structural string (SMILES/InChI) or empirical formula is provided in the current listing. Without these, definitive assignment of functional groups, ring systems, or stereochemistry cannot be made.

General guidance (chemistry/literature context)

• For small-molecule library members like L343, the structure is typically disclosed on the certificate of analysis (CoA) or a library manifest, including key identifiers (IUPAC name, SMILES, exact mass, and potential stereochemical descriptors).
• If you intend to use L343 in structure–activity relationship (SAR) studies, ensure you obtain a structure-verified batch record and, where applicable, NMR/LC–MS confirmation to match the CAS reference material.

Synthetic Utility

Without the structure of L343, specific synthetic roles or transformations cannot be assigned.

• Item-specific functional groups/reactivity: Not specified for this item; refer to CoA/Spec Sheet.

General guidance if structure is available (non-item-specific)

• Building-block assessment: Identify handles (halides, boron, stannanes, alkenes/alkynes, carbonyls, amines) for cross-coupling, homologation, or derivatization.
• Protection strategy: If multifunctional, plan orthogonal protection to enable late-stage diversification.
• Late-stage functionalization: Consider C–H activation, photoredox, or Minisci-type modifications if the scaffold supports such chemistry.

If your intent is synthetic elaboration rather than screening, request the structural file and any available analytical data to ensure compatibility with your route.

Target Specificity

No biological target, binding specificity, or selectivity information is provided for L343 in the current listing.

• Item-specific target data: Not specified for this item; refer to CoA/Spec Sheet or associated library documentation.

If L343 is part of a themed library (e.g., kinase-focused, GPCR-biased), consult the library manifest or screening guide for putative target classes. Validate any apparent specificity with orthogonal assays and concentration–response studies to rule out nonspecific effects.

Frequently Asked Questions

How should this product be stored?

Store at ?20 °C. Freezer storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 1443520-22-6, the molecular formula is C51H95NO6, and the molecular weight is 818.30 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.