Lactobacillus Agar Medium - BioReagent

Cat. No.: L1528466

GRADE & PURITY BioReagent

Storage

Room temperature

Shipped In

Normal

Overview

Lactobacillus Agar Medium preparation method: Weigh 45.5 g of this product in 1 L of distilled or deionized water (the volume may be scaled up or down proportionally), dissolve with gentle warming, and dispense; autoclave at 121°C for 15 minutes; after sterilization, shake well to prevent agar from settling and solidifying at the bottom of the vessel, then set aside for use. Lactobacillus Agar Medium formula (per liter) contains: Peptonized milk 15.0 g (provides nitrogen source and growth factors), yeast extract powder 5.0 g (supplements B vitamins and amino acids), tomato extract powder 2.5 g (provides specific growth-promoting factors), glucose 10.0 g (serves as the primary carbon and energy source), potassium dihydrogen phosphate 2.0 g (maintains pH buffering system), Tween 80 1.0 g (acts as an emulsifier and fatty acid source to promote lactobacillus growth), and agar 10.0 g (serves as a solidifying agent); final pH adjusted to 6.8 ± 0.2 (25°C).

Specifications

Specifications & Purity

BioReagent

Storage

Room temperature

Shipped In

Normal

Grade

BioReagent

Product Line

biological_products

Application Protocols

The following are general, literature-based protocols for dehydrated Lactobacillus agar media and are not item-specific. Adjust steps to your lab SOP and the CoA/Spec Sheet.

A. Plate Preparation (general)

• Weigh the recommended grams per liter (Not specified for this item; refer to CoA/Spec Sheet.).
• Suspend in purified water with stirring; heat gently to dissolve completely.
• Autoclave at 121 °C for 15 min. Allow to cool to 45–50 °C.
• If method requires, aseptically add heat-labile supplements (sterile-filtered). Mix gently.
• Pour into sterile Petri dishes; allow to solidify. Store plates inverted at 2–8 °C if not used immediately (duration per SOP).

B. Spread Plate Enumeration (general)

• Prepare serial dilutions of sample in appropriate diluent.
• Plate 0.1 mL aliquots on solidified plates; spread evenly with sterile spreader.
• Incubate inverted plates at species-appropriate temperature (e.g., 30–37 °C) under microaerophilic/anaerobic conditions as required.
• Count colonies (25–250 CFU/plate target range) and calculate CFU per mL or g.

C. Streak Isolation (general)

• Streak sample onto plate for single colonies using aseptic technique.
• Incubate as above; select well-isolated colonies for downstream testing.

Exact grams per liter, supplements, and incubation parameters for this item: Not specified; refer to CoA/Spec Sheet.

Biological Roles

This section summarizes biological context relevant to the intended organisms (lactic acid bacteria) rather than the medium itself.

• Lactobacillus spp. are Gram-positive, facultatively anaerobic or microaerophilic bacteria that primarily perform homo- or heterofermentative metabolism of carbohydrates to produce lactic acid (literature, general).
• The acidic end-products contribute to self-selectivity of low-pH media and suppression of many competing microbes. Nutrient profiles favor amino acid/peptide utilization and vitamin-dependent growth (literature, general).
• Applications in research include studying carbohydrate utilization pathways, stress tolerance (low pH, bile salts depending on strain), and bio-preservation mechanisms in fermented foods.
• For plate-based work, colony morphology, acidification of the surrounding medium, and growth temperature preferences (often 30–37 °C, species-dependent) are common phenotypic readouts (literature, general).

Note: The medium composition in this catalog item is not specified; selectivity and performance toward particular Lactobacillus species will depend on the exact formulation and any supplements used. Always validate growth with your target strains and consult the CoA/Spec Sheet for lot-specific performance notes.

Buffer Applications

Not typically applicable. Lactobacillus Agar Medium is a nutrient medium reconstituted with water and sterilized; it is not used as a stand-alone buffer system.

Practical note (literature, general): The prepared medium will have a formulation-defined pH that contributes to selectivity. If pH adjustment is required for a specific method, do so prior to sterilization and in alignment with method references. Exact pH targets for this item: Not specified; refer to CoA/Spec Sheet.

Green Alternatives

As an aqueous, agar-based microbiological medium, environmental considerations center on energy use, water use, and plastic waste rather than solvent toxicity.

Greener practice suggestions (literature, general):

• Energy efficiency: Optimize autoclave loads and cycles; avoid under-filled runs. Use validated, shortest effective sterilization times to reduce energy consumption.
• Waste reduction: Prepare only the volume required. Consolidate plate pouring sessions to minimize leftover molten medium. Reuse glassware and switch from single-use plastics to reusable glass plates and bottles where permitted.
• Water stewardship: Use appropriate water quality without over-specifying (e.g., Type II rather than Type I) to reduce purification energy.
• Inventory management: Store tightly sealed at room temperature as recommended to extend shelf life and prevent caking, thereby reducing discards.

Comparison (literature, general):

• Dehydrated media vs. ready-to-use plates: Dehydrated media reduce shipping mass/volume and can lower packaging waste, but require on-site sterilization energy. Ready-to-use plates save on autoclave energy but increase packaging and cold-chain burdens in some cases.

Item-specific eco-certifications, recycled content, or take-back programs: Not specified for this item; refer to CoA/Spec Sheet.

Pharmaceutical Uses

No therapeutic or clinical claims are made. For research use only.

Relevant laboratory/industrial roles (literature, general):

• Microbiological quality control (non-clinical research and development): cultivation and enumeration of lactic acid bacteria in fermentation development labs and raw-material assessments.
• Process development: monitoring starter cultures and assessing robustness of lactic fermentations in food, nutraceutical, or bioprocess R&D settings.
• Environmental monitoring (R&D environments): recovery of lactic acid bacteria from surfaces or air samples when appropriate swabs or contact plates are prepared with selective media.

Compendial/standard references: Specific pharmacopeial status for this exact product is not provided. If compendial compliance is required, verify against the CoA/Spec Sheet and relevant monographs/methods (e.g., growth promotion tests per USP/EP where applicable). Item-specific certifications/monograph alignment: Not specified for this item; refer to CoA/Spec Sheet.

Physical Properties

Item-specific physico-chemical specifications for this dehydrated medium are not provided in the product data.

• Appearance (dehydrated): Not specified for this item; refer to CoA/Spec Sheet. (General literature: typically a free-flowing beige/tan powder.)
• Solubility: Water-soluble as a mixture; forms a suspension/solution prior to sterilization (literature, general). Insoluble in organic solvents.
• pH (of prepared medium): Not specified for this item; refer to CoA/Spec Sheet. (Literature, typical Lactobacillus agar/MRS agar final pH at 25 °C ≈ 5.5–6.5 depending on formulation.)
• Melting/boiling point: Not applicable to mixtures; prepared medium solidifies by gelation upon cooling (agar gel point ~32–40 °C; literature, general).
• Density (powder) / Refractive index / UV cutoff / Conductivity: Not specified for this item; refer to CoA/Spec Sheet.
• Hygroscopicity: Dehydrated media are commonly moisture-sensitive; keep container tightly closed to prevent caking and variability (literature, general).

Note: Physical properties of complex media depend on exact composition and lot. Always consult the CoA/Spec Sheet for the lot-specific appearance, target pH after reconstitution, and performance characteristics.

Quality & Grades

• Grade: BioReagent (as provided). In the context of microbiological media, BioReagent indicates suitability for laboratory research workflows, with attention to lot-to-lot consistency and performance characteristics appropriate for culture work. It does not imply clinical or diagnostic use.
• Item-specific QC parameters: Not specified for this item; refer to CoA/Spec Sheet.

Typical quality attributes for dehydrated media (literature, general):

• Appearance and flow: free-flowing, homogeneous powder without hard lumps.
• Performance tests: growth promotion testing with representative Lactobacillus strains (e.g., colony size, morphology, and recovery within defined incubation times); sterility of uninoculated prepared medium.
• pH after reconstitution/sterilization: lot-specific acceptance range.

Implications for users:

• BioReagent media are intended for research cultivation, enumeration, and maintenance of target organisms under controlled lab conditions.
• For regulated environments (QC, environmental monitoring), verify that the lot-specific CoA documents growth-promotion test results that meet your internal or compendial criteria.
• Stabilizers/preservatives: Not specified for this item; refer to CoA/Spec Sheet.

Note: Always verify lot-specific details (recommended grams per liter, target final pH, and performance) on the CoA before method validation or comparison studies.

Reaction & Applications

Chemical reaction usage is not applicable; however, application in microbiology is central.

Research applications (literature, general):

• Selective cultivation and enumeration of Lactobacillus spp. from fermented foods, probiotics, and environmental or process samples, leveraging acidic pH and nutrient composition favorable to lactic acid bacteria.
• Strain maintenance: streak plating for colony isolation and periodic subculture to maintain strain libraries.
• Quality control: growth promotion testing, bioburden assessments of materials with expected lactic acid bacterial content, and verification of fermentation processes.
• Colony morphology studies: discrimination of lactic acid bacteria based on colony size, texture, and acid production halos on certain indicator-containing variants (if applicable; formulation-dependent).

Practical tips (literature, general):

• Thoroughly suspend/deagglomerate powder before heating. Avoid localized scorching by using gentle stirring and gradual heating to achieve complete dissolution.
• Autoclave using validated cycles (e.g., 121 °C) and avoid excessive sterilization times that can darken media and degrade nutrients.
• Cool to ~45–50 °C before adding heat-labile supplements (if used) and pouring plates to minimize condensation and preserve activity.

Exact selectivity and supplement requirements for specific Lactobacillus species/strains: Not specified for this item; consult CoA/Spec Sheet and method references.

Reaction Conditions

Chemical reaction conditions are not applicable. The following preparation parameters are provided as general microbiological guidance (literature, typical) and are not item-specific.

• Reconstitution: Dehydrated Lactobacillus-selective agar media are commonly prepared at approximately 50–60 g per liter of purified water, heated with stirring until fully dissolved (literature, typical for MRS/Lactobacillus agar ≈ 55 g/L). Exact concentration for this item: Not specified; refer to CoA/Spec Sheet.
• Sterilization: Autoclave at 121 °C for 15 minutes (gravity or pre-vacuum cycle as validated). Avoid prolonged sterilization that may degrade nutrients (literature, general).
• Post-sterilization handling: Cool to ~45–50 °C; add sterile-filtered, heat-labile supplements if required by method; mix gently to avoid bubbles.
• Pouring plates: Dispense into sterile Petri dishes (commonly 20–25 mL for 90-mm plates) and allow to solidify on a level surface. Dry plates briefly (laminar flow) if excess condensation forms (literature, general).
• Incubation of inoculated plates: Species- and method-dependent; many Lactobacillus spp. grow at 30–37 °C under microaerophilic or anaerobic conditions for 24–72 h (literature, general). Exact conditions depend on strain and formulation.

Always prioritize the CoA/Spec Sheet for item-specific grams per liter, target final pH, and any supplement requirements.

Safety & Handling

GHS classification and hazard statements are not provided in the product data for this item.

• GHS/Classification: Not specified for this item; refer to SDS.
• Anticipated hazards (general): Low acute toxicity expected for dehydrated culture media; nuisance dust may cause mechanical irritation to eyes/respiratory tract. After inoculation and incubation, plates and broths should be treated as biohazardous (literature, general).
• PPE: Wear lab coat, safety glasses, and appropriate gloves. Use dust control (avoid aerosolization) when weighing. Handle inoculated media within appropriate biosafety practices (BSL-1/2 as applicable to organism and local policy).
• Storage incompatibilities: Avoid moisture and strong oxidizers. Keep container tightly sealed; protect from humidity to maintain performance (literature, general).
• First aid (overview): If inhaled, move to fresh air. If on skin/eyes, rinse with water. If ingested, rinse mouth and seek medical advice if symptoms persist. Always follow SDS guidance.
• Sterilization/pressure risks: During autoclaving, do not overfill vessels; use vented caps and avoid tightly capped bottles to prevent pressure buildup. Allow media to cool before pouring plates.

Always consult the product’s SDS and institutional biosafety procedures for authoritative safety information. For research use only.

Solvent Selection

This product is a dehydrated microbiological medium intended to be reconstituted in water; traditional solvent-selection considerations for chemical reactions are not applicable.

Practical guidance (literature, general):

• Use purified water (e.g., ASTM Type II or better) for reproducibility. For demanding microbiology, sterile water for injection (WFI) grade is unnecessary; instead, prepare with purified water and sterilize the medium by autoclaving.
• Avoid high-ionic-strength or buffered waters unless validated, as they may alter the target pH and gel strength.
• For plate media, reconstitute with water per label/CoA instructions, heat to dissolve, then sterilize. Agar provides the solid matrix upon cooling; no organic solvents are required.

Alternatives/adjuncts:

• Filtration sterilization is generally not recommended for agar-containing media due to viscosity; use autoclaving instead (literature, general).

Item-specific reconstitution volumes and grams per liter: Not specified for this item; refer to CoA/Spec Sheet.

Storage & Reconstitution

• Storage conditions (as provided): Room temperature. Keep container tightly closed and protected from moisture. Shipped under normal conditions.
• Shelf life / Retest date: Not specified for this item; refer to CoA/Spec Sheet.

Reconstitution and use (literature, general; not item-specific):

• Reconstitution: Add the specified grams of dehydrated medium to purified water to reach the target volume (exact g/L Not specified; see CoA/Spec Sheet). Stir to fully disperse and heat to dissolve.
• Sterilization: Autoclave at 121 °C for 15 minutes unless otherwise specified. Do not over-sterilize.
• Post-sterilization: Cool to 45–50 °C before pouring plates or adding sterile, heat-labile supplements.
• Storage of prepared plates: Store inverted in sealed sleeves to minimize drying, typically at 2–8 °C (duration per your SOP/validation). Inspect for contamination, dehydration, or pH/appearance changes before use.
• Freeze–thaw: Not applicable to dehydrated media; avoid freezing prepared agar plates unless validated, as gel structure and performance can be compromised.

Always consult the lot-specific CoA/Spec Sheet and SDS for precise preparation parameters, target final pH, and performance criteria. For research use only.

Structure & Identity

Lactobacillus Agar Medium (SKU: L1528466) is a complex, multi-component microbiological growth medium formulated for the cultivation of Lactobacillus spp. and related lactic acid bacteria. As a dehydrated blend, it does not have a single defined molecular structure.

• Product type: Complex culture medium (dehydrated powder) for agar plate preparation (research use only)
• Molecular formula: Not applicable to complex blends (Not specified for this item; refer to CoA/Spec Sheet.)
• Molecular weight: Not applicable to complex blends (Not specified for this item; refer to CoA/Spec Sheet.)
• SMILES / InChI / InChIKey: Not applicable (mixture)
• Structural features: Contains multiple functional groups across amino acids/peptides, carbohydrates, minerals, and gelling agent(s) typical for agar media (literature, general). No single 2D structure describes the product.
• 2D description (general): Heterogeneous mixture of nutrients (nitrogen sources such as peptides/amino acids; carbon sources such as sugars), growth factors (vitamins), mineral salts, and a polysaccharide gelling matrix when prepared with agar (literature, general).

Item-specific composition, component percentages, and target pH: Not specified for this item; refer to CoA/Spec Sheet.

Synthetic Utility

Not applicable. Lactobacillus Agar Medium is a biological culture medium and is not employed as a reagent or building block for chemical synthesis. For practical use, see Reaction & Applications, Application Protocols, and Storage & Reconstitution.

Target Specificity

Item-specific target information (e.g., selectivity spectrum, required supplements, or confirmed species recovery) is not provided in the product data.

• Not specified for this item; refer to CoA/Spec Sheet.

General note (literature): Media marketed for Lactobacillus typically favor lactic acid bacteria via acidic pH, carbohydrate sources, and growth factors. Actual selectivity across Lactobacillus species and against non-target flora depends on exact formulation and incubation conditions.