LC-MB12 - Moligand™, 10 mM in DMSO , CAS No.2828438-38-4

CAS: 2828438-38-4 Cat. No.: L1497629 Formula: C₄₃H₄₄Cl₂N₁₀O₈ Molecular Weight: 899.78

GRADE & PURITY Moligand™, 10 mM in DMSO

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

Overview

LC-MB12 is an orally active PROTAC compound targets FGFR2 degradation with a DC 50 of 11.8 nM. LC-MB12 contains BGJ398 (a FGFR2 inhibitor ), PROTAC linker and CRBN.LC-MB12 inhibits FGFR2 signaling in gastric cancer cells and has anti-tumor activity.

Specifications

Specifications & Purity

Moligand™, 10 mM in DMSO

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

Moligand™

Action Type

INHIBITOR

Names and Identifiers

Molecular Weight

899.78

Application Protocols

While no item-specific validated protocol is provided, the following generic screening workflow is commonly used for small-molecule library members.

DMSO stock preparation

• Equilibrate vial to room temperature in a desiccated environment.
• Add anhydrous DMSO to achieve a 10–20 mM stock; vortex and, if needed, sonicate briefly.
• Record batch ID, preparation date, and calculated concentration.

Assay dilution (biochemical)

• Prepare assay buffer and match DMSO % between test and control wells (commonly 0.1–1.0% v/v).
• Add compound to achieve desired test concentrations (e.g., 10-point 3-fold serial dilutions).
• Include positive/negative controls and counterscreens to detect assay interference.

Assay dilution (cell-based)

• Verify cell-line DMSO tolerance; typically keep ≤0.1–0.5% v/v.
• Inspect for precipitation or phase separation; if present, reduce top concentration or add compatible co-solvent per assay rules.

Storage of working solutions

• Aliquot to minimize freeze–thaw cycles; store at the recommended temperature (see Storage & Reconstitution).

Biological Roles

Biological function, targets, and pathways for LC-MB12 are not disclosed. As a coded Moligand™ library member, it is intended for exploratory research.

• Known targets/activities: Not specified for this item; refer to project-specific disclosures or SDS/CoA if available under agreement.
• Assay contexts: Suitable for use in biochemical binding/enzymatic assays and compatible cell-based readouts provided solubility and cytotoxicity are empirically verified.

General considerations for small-molecule library members

• Verify cytotoxicity profiles in the relevant cell model before phenotypic screening.
• Assess pan-assay interference potential (e.g., aggregator behavior, redox cycling) using orthogonal assays.
• For thermal shift (DSF/DSC) or SPR, optimize buffer composition and DMSO matching between sample and reference.

No clinical or therapeutic claims are made; this product is for research use only.

Buffer Applications

LC-MB12 is not a buffering agent, and no acid/base properties are disclosed. Buffer selection should therefore be driven by the requirements of the biological or biochemical assay rather than any intrinsic buffering capacity of LC-MB12.

General guidance (assay-focused)

• Use robust buffers such as HEPES (pH 7.0–8.0), PBS (pH ~7.4), or Tris (pH 7.0–9.0) as appropriate for your target system.
• Match DMSO percentage in all wells, including controls, to negate solvent effects.
• Include nonionic surfactant (0.01–0.05%) if precipitation is observed upon dilution.

Buffer recipes or pKa values specific to LC-MB12 are not applicable.

Green Alternatives

Green chemistry considerations primarily pertain to the choice of handling solvents and assay media rather than the compound itself (structure undisclosed).

Greener solvent choices (general guidance)

• Replace high-toxicity solubilizers where possible. For dilution from DMSO stocks, consider:
  • Ethanol or isopropanol as intermediate co-solvents instead of DMF/NMP when compatible with the assay.
  • Aqueous buffers with small amounts of biocompatible surfactant (e.g., polysorbates) to minimize total organic content.

Comparison (general)

• DMSO vs DMF/NMP: DMSO has a more favorable EHS profile but strong odor; DMF/NMP carry higher reproductive toxicity concerns.
• ACN vs MeOH/EtOH: MeOH/EtOH are generally preferred for EHS; choose ACN for LC-MS compatibility only when necessary.

Operational tips

• Use miniaturized assay volumes (HTS formats) to reduce solvent usage.
• Consolidate stock concentrations to minimize total DMSO mass moved and stored.
• Employ closed, low-evaporation plates to limit VOC emissions.

Note: No greener “alternative compound” can be proposed without structural or functional context for LC-MB12.

Pharmaceutical Uses

This product is not supplied as an excipient or therapeutic and carries a research-use-only designation.

• Regulatory/Pharmacopeia status: Not applicable; not intended for human or veterinary use.
• Formulation role: None specified. Not specified for this item; refer to CoA/Spec Sheet if a specific salt/form is provided for handling.

Context (general)

• If hits derived from LC-MB12 progress, any preformulation or developability assessments (solubility, stability, polymorph) must be newly generated for that chemotype; no assumptions should be made from this listing.

Physical Properties

Item-specific physicochemical constants have not been disclosed for LC-MB12. Typical screening use focuses on solution handling rather than bulk physical characterization.

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Melting/Boiling Point: Not specified for this item; refer to CoA/Spec Sheet.
• Density/Refractive Index: Not specified for this item; refer to CoA/Spec Sheet.
• Solubility: Not specified for this item; refer to CoA/Spec Sheet.
• logP/logD, pKa: Not specified for this item; refer to CoA/Spec Sheet.

Practical handling guidance (general/literature-based)

• For small-molecule library members, initial dissolution in anhydrous DMSO (analytical or screening grade) at 10–20 mM is commonly used (general practice).
• If aqueous assays are required, perform stepwise dilution of the DMSO stock into assay buffer with gentle vortexing; include co-solvent controls to monitor precipitation (general practice).
• When compound identity/structure is confidential, avoid estimating pKa or logP from unrelated analogs; instead, empirically determine solubility limits in your assay matrix (general practice).

Quality & Grades

This item is supplied as part of the Moligand™ collection, a coded small-molecule library intended for discovery workflows.

• Grade/Purity: Moligand™. Specific purity metrics (e.g., HPLC area %, residual solvents, water content) are not disclosed here. Not specified for this item; refer to CoA/Spec Sheet.
• Intended use: In vitro research, screening, and SAR exploration under research-use-only conditions.

What Moligand™ generally implies (general context)

• Library members are typically curated for screenability (stock solution behavior, compatibility with common assay solvents) and traceability (coded IDs, batch records).
• Documentation available on request may include batch CoA (purity method, ID method) and SDS.

What is item-specific

• Any stabilizers, counter-ions/salt forms, or form factors (neat solid vs DMSO solution) are: Not specified for this item; refer to CoA/Spec Sheet.

Recommendations

• Verify assay-interferent checks (e.g., detergent sensitivity, redox activity, fluorescence interference) during QC-in-use.
• For chromatography-heavy workflows (LC-MS), confirm background cleanliness using a blank injection of your reconstitution solvent.

Reaction & Applications

LC-MB12 is supplied as a screening library compound rather than a synthetic reagent; therefore, classical reaction applications (e.g., as a catalyst, building block, or solvent) are not the primary use.

Research applications (general for small-molecule libraries)

• Primary/secondary screening against biochemical or cell-based targets.
• Hit confirmation and orthogonal assays for artifact reduction (e.g., counterscreens for aggregation, redox cycling, or fluorescence interference).
• Mechanism-of-action hypotheses generation followed by target deconvolution (e.g., chemoproteomics or thermal shift assays), contingent on structure disclosure under appropriate agreements.
• SAR by catalog: pairing LC-MB12 with near neighbors from the same collection if available.

Practical tips

• Use fresh DMSO master stocks and limit freeze–thaw cycles to maintain integrity.
• Track assay interference flags (absorbance/fluorescence overlap, detergent sensitivity) and incorporate controls.
• Confirm chemical stability under assay conditions (pH, redox, light) using LC-MS when possible.

Note: Without disclosed structure, no reaction mechanism or named-reaction participation can be assigned to LC-MB12.

Reaction Conditions

Classical reaction condition guidance is not applicable to LC-MB12 as it is not intended to serve as a reagent, catalyst, or substrate in synthetic transformations.

Assay preparation conditions (general practice)

• Prepare 10–20 mM DMSO stocks at room temperature, with optional brief sonication.
• For biochemical assays, typical final DMSO concentration is controlled to ≤1% v/v unless otherwise validated.
• For cell-based assays, consider ≤0.1–0.5% v/v DMSO and verify tolerability in your model system.
• Use amber vials or subdued lighting if the compound is later found to be light sensitive (confirm via CoA or stability screens).

No literature yields, catalysts, or temperature profiles are applicable for this undisclosed library member.

Safety & Handling

Hazard classification for LC-MB12 is not provided in the open listing. Always consult the product-specific SDS before use.

• GHS Classification: Not specified for this item; refer to SDS.
• Signal Word / H-Statements / Pictograms: Not specified for this item; refer to SDS.

General laboratory precautions (best practice)

• Wear appropriate PPE: lab coat, safety glasses, and gloves suitable for organic small molecules (e.g., nitrile).
• Handle powders/films in a fume hood; minimize aerosolization and dust.
• Avoid contact with strong oxidizers or reducing agents unless compatibility is confirmed.
• In case of skin/eye contact: rinse with water for at least 15 minutes and seek medical attention; if inhaled, move to fresh air; if ingested, seek medical advice and present SDS.
• Prevent contamination of common reagent stocks; dedicate pipette tips for DMSO stock handling.

Waste and spills

• Collect waste in properly labeled organic waste containers per institutional guidelines.
• For small spills, absorb with inert material (e.g., vermiculite) and dispose per SDS guidance.

Note: Hazard properties can vary widely across library members; the SDS is the authoritative source.

Solvent Selection

Because the exact structure of LC-MB12 is not disclosed, solvent choice should be guided by empirical solubility screening.

Practical approach

• Start with anhydrous DMSO to prepare a 10–20 mM master stock. Warm gently (≤40°C) and vortex/sonicate to aid dissolution.
• If DMSO is insufficient, evaluate co-solvents: DMF, NMP, ethanol, isopropanol, acetonitrile (general screening practice). Avoid halogenated solvents in biological contexts.
• For aqueous use, dilute the DMSO stock into buffer containing 0.01–0.1% nonionic surfactant (e.g., Tween 20 or Pluronic F-68) to mitigate precipitation, validating assay compatibility.

Small comparison (general)

• DMSO: highest success rate for diverse chemotypes; strong water miscibility; common in HTS.
• DMF/NMP: powerful solubilizers; verify cytotoxicity/assay tolerance.
• ACN/EtOH/IPA: miscible cosolvents that can reduce DMSO percentage in final assays.

Notes

• Record final organic co-solvent percentage in assays (commonly ≤0.5–1.0% v/v for cells; higher may be acceptable for biochemical assays).
• Filter stocks only if known to be stable and non-adsorptive; otherwise, use centrifugation to remove particulates.

Storage & Reconstitution

Follow the item-specific storage guidance to preserve integrity of LC-MB12 for screening use.

• Storage Conditions: Store at -80°C.
• Shipped In: Dry ice packs + Cold packs to maintain low temperature during transit.
• Form at receipt: Not specified for this item; refer to CoA/Spec Sheet (neat solid vs pre-dissolved solution not disclosed).

Reconstitution (general guidance)

• If supplied neat, allow the sealed container to equilibrate to room temperature in a desiccator before opening to avoid condensation.
• Dissolve in anhydrous DMSO to prepare a 10–20 mM master stock unless otherwise stated on the CoA.
• Filter sterilization is generally not recommended for scarce library compounds; if sterility is required, use 0.2 µm PTFE filters and verify compound recovery.

Aliquoting and stability

• Prepare single-use aliquots to avoid repeated freeze–thaw. Store aliquots at -80°C.
• Protect from moisture and light until stability is characterized.

Labeling

• Record SKU (L1497629), lot/batch, concentration, solvent, and preparation date on all aliquots.

Note: For any item-specific stability (e.g., hygroscopicity, light sensitivity), consult the CoA/Spec Sheet and SDS.

Structure & Identity

A member of Aladdin's Moligand screening collection, LC-MB12 is supplied for discovery research where the detailed structure is not publicly disclosed.

• SKU: L1497629 (LC-MB12)
• Collection/Grade: Moligand™ small-molecule library member
• CAS: 2828438-38-4
• Molecular Formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular Weight: Not specified for this item; refer to CoA/Spec Sheet.
• SMILES: Not specified for this item; refer to CoA/Spec Sheet.
• InChI / InChIKey: Not specified for this item; refer to CoA/Spec Sheet.

Structural features

• Detailed functional groups, ring systems, and stereochemistry are not disclosed for this library item. If structural disclosure is required for your workflow (e.g., computational models, target deconvolution), please request the item-specific CoA/Spec Sheet under your confidentiality framework.

2D structure (descriptive)

• Not available in the open catalog for LC-MB12; it is supplied as a coded screening entity within the Moligand™ collection.

Synthetic Utility

LC-MB12 is distributed as a finished library compound for screening, not as a building block or reagent. Without a disclosed structure, no synthetic transformations, protecting-group strategies, or retrosynthetic roles can be assigned.

General note

• For follow-up chemistry on an identified hit, request structural disclosure through the appropriate channels, then plan SAR exploration using standard medicinal chemistry tactics (scaffold modification, vector exploration, property tuning).

Target Specificity

Target information for LC-MB12 is not disclosed in this catalog entry.

• Intended biological targets or selectivity profiles: Not specified for this item; refer to project-specific data if provided under confidentiality.

For screening workflows, incorporate broad counterscreens to rule out nonspecific mechanisms before committing resources to target deconvolution.

Frequently Asked Questions

What is Moligand??

Moligand? is one of Aladdin Scientific's premium product lines, formulated for high-purity applications across multiple workflows. Compared to standard grades in the same workflow, Moligand? products typically offer tighter specifications, more rigorous QC, and stronger lot-to-lot consistency.

How should this product be stored?

Store at ?80 °C. Ultra-low temperature storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 2828438-38-4, the molecular formula is C43H44Cl2N10O8, and the molecular weight is 899.78 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.