Lichen Red Ethanol Solution (1%) - BioReagent,Suitable for microbiology,Biological Stain,for microscopy,1% , CAS No.1400-62-0

CAS: 1400-62-0 Cat. No.: L1508167 Molecular Weight: 500.5 Beilstein Registry Number: 6885 EC Number: 215-750-6 PubChem CID: 72685

GRADE & PURITY BioReagent, Suitable for microbiology, Biological Stain, for Microscopy, 1%

Synonyms

Orcein Ethanol Solution | Orcein Ethanol Staining Solution

Storage

Store at 2-8°C,Protected from light

Shipped In

Wet ice

Application

Microbial staining

Overview

  Orcein, also known as lichenin, has a CAS number of 1400-62-0 and a molecular weight of 500.51. It is a biological mordant that can be used for staining HBsAg, flagella, and other structures.

  Lichen Red Ethanol Solution (1%), also known as Acidified Orcein Staining Solution, is mainly prepared from orcein, hydrochloric acid, ethanol, deionized water and other components. The solution is acidic, featuring simple operation and high specificity, while the staining effect may vary with different batches of raw materials. It can be used for the staining of Hepatitis B Virus Surface Antigen (HBsAg), as well as the staining of lipofuscin and elastic fibers. This reagent is intended for research use only and not for clinical diagnosis or any other purposes.

Operating Procedures (For Reference Only):

1. Fix the tissues in 10% neutral formalin fixative, followed by routine dehydration and embedding.

2. Cut the sections at a thickness of 4 µm, then perform routine dewaxing to water with xylene or environment-friendly dewaxing and clearing solution.

3. Immerse the sections in acidified potassium permanganate solution (self-prepared) for oxidation for 5 minutes, then rinse briefly with water.

4. Transfer the sections into 1% oxalic acid solution (self-prepared) for bleaching for 2 minutes to remove the coloration caused by potassium permanganate, followed by rinsing under running water for 2 minutes.

5. Rinse the sections briefly in 70% ethanol.

6. Place the sections into orcein ethanol solution, cover them, and stain for 5 hours or longer.

7. Rinse the sections twice in 70% ethanol, 10–30 seconds each time, until no staining solution elutes from the sections.

8. Conduct routine dehydration, clear the sections with xylene or environment-friendly dewaxing and clearing solution, and mount the sections with neutral gum.

Precautions:

1. The staining effect may vary depending on different batches of raw materials.

2. For your safety and health, please wear a lab coat and disposable gloves during operation.

3. Please use the reagent as soon as possible after opening to avoid affecting the results of subsequent experiments.

Specifications

Synonyms

Orcein Ethanol Solution | Orcein Ethanol Staining Solution

Specifications & Purity

BioReagent, Suitable for microbiology, Biological Stain, for microscopy, 1%

Stability And Storage

Store at 2-8℃ long term (12 months). Store in the dark.

Storage

Store at 2-8°C, Protected from light

Shipped In

Wet ice

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

Biological Stain, BioReagent, for Microscopy, Suitable for microbiology

Names and Identifiers

• Isomeric SMILES: CC1=CC(=CC(=C1NC2=C(C3=C(C=C2O)OC4=CC(=O)C(=NC5=C(C=C(C=C5C)O)O)C(=C43)C)C)O)O
• WGK Germany: 3
• PubChem CID: 72685
• Molecular Weight: 500.5
• Beilstein: 6885