Lipopolysaccharide (LPS) Solution (500X) - sterile-filtered,BioReagent,500X (1mg/mL)

Cat. No.: L1508370

GRADE & PURITY Sterile-filtered, BioReagent, 500X (1mg/mL)

Synonyms

LPS Solution (500X) | LPS

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Application

Flow Cytometry

Overview

  This product is a sterile-filtered, concentrated stock solution of highly purified Lipopolysaccharide (LPS) derived from E. coli serotype 055:B5. At a concentration of 1 mg/mL, it is formulated as a 500X stock. A simple 500-fold dilution yields a standard working solution of 2 µg/mL, greatly facilitating dose control and operation in cell stimulation experiments. The product undergoes rigorous validation for endotoxin activity and exhibits high batch-to-batch consistency. It is suitable for research in immunology, inflammation, and cell signaling, specifically for stimulating immune cells such as macrophages and monocytes to induce cytokine secretion.

Key Components

• Active Ingredient: Lipopolysaccharide (LPS), Source: E. coli 055:B5

• Solvent: Sterile, Pyrogen-free Cell Culture Grade Water

• Concentration: 1 mg/mL (500X Stock Solution)

Features

• High Purity & Activity: Purified to possess high biological activity, effectively activating the TLR4 signaling pathway.

• Ready-to-Use Stock: Pre-formulated as a 500X concentrated solution at 1 mg/mL, eliminating the need for weighing and dissolving, thereby reducing contamination risks.

• Convenient: Easily diluted to the desired working concentration (e.g., 1X = 2 µg/mL), ideal for dose-response studies.

• Sterile-Filtered: Solution is sterile-filtered through a 0.22 µm membrane, ready for direct addition to cell cultures.

Procedure

1. Thawing & Dilution: Thaw the solution at room temperature or 4°C prior to use. Gently vortex to mix.
2. Working Solution Preparation: Dilute this stock solution with sterile cell culture medium, PBS, or saline according to experimental needs.
   2.1 Standard 1X Working Concentration: 2 µg/mL. For example, add 2 µL of this stock to 998 µL of medium and mix.
   2.2 Common Working Range: For most cell stimulation assays, the final LPS concentration typically ranges from 0.1 ng/mL to 2 µg/mL (i.e., 0.00005X to 1X). Optimization based on specific cell type and research objective is recommended.
3. Cell Stimulation: Add the prepared LPS working solution directly to the cell culture system, mix gently, and incubate. Stimulation time usually varies from 2 to 24 hours, depending on the readout (e.g., cytokine secretion, signaling protein detection).
4. Note: To preserve optimal activity, it is advised to aliquot the original stock into small volumes (e.g., 10-20 µL/tube) and store at -20°C. Diluted working solutions should be prepared fresh for each use.
   

Precautions

1. Safety First: This is a biologically active material. Always follow strict biosafety protocols during handling. Avoid creating aerosols.

2. Avoid Contamination: Perform dilutions under sterile conditions using sterile reagents and consumables.

3. Avoid Freeze-Thaw Cycles: Repeated freezing and thawing may lead to loss of activity or precipitation. Aliquotting is strongly recommended.

4. Experiment Optimization: Sensitivity to LPS varies significantly among different cell lines or primary cells. For initial use, a concentration gradient (e.g., 0.1, 1, 10, 100, 1000 ng/mL) is recommended to determine the optimal stimulation dose.

5. Intended Use: For research use only. Not for use in diagnostic or therapeutic procedures.

Specifications

Synonyms

LPS Solution (500X) | LPS

Specifications & Purity

sterile-filtered, BioReagent, 500X (1mg/mL)

Stability And Storage

Aliquot upon receipt and store frozen at -20°C(12 months). Avoid repeated freeze-thaw cycles.

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

BioReagent, Sterile-filtered

Application

• Application: Dilution info
• Flow Cytometry:

  1/500;