Lysozyme Solution (10 mg/mL) - BioReagent,Suitable for molecular biology,10 mg/mL

Cat. No.: L1520263

GRADE & PURITY Suitable for molecular biology, BioReagent, 10 mg/mL

Synonyms

Muramidase Solution

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Application

DNA Extraction

Overview

Lysozyme, also known as muramidase, is an alkaline enzyme capable of hydrolyzing the mucopolysaccharides present in pathogenic bacteria. Its mechanism of action involves breaking the β-1,4 glycosidic bonds between N-acetylmuramic acid and N-acetylglucosamine in the bacterial cell wall. This breaks down the insoluble mucopolysaccharides in the cell wall into soluble glycopeptides, leading to cell wall rupture, leakage of cellular contents, and ultimately, bacterial lysis.

The lysozyme solution (10 mg/mL) is composed of lysozyme and TRIS among other components, with a pH of approximately 8.0. It is primarily used to lyse bacterial cell walls to facilitate subsequent plasmid DNA extraction procedures.

This reagent is intended for research purposes only and is not suitable for clinical diagnosis or other uses.

Product Features

• High heat resistance, maintaining activity within a certain temperature range.
• Insensitive to organic solvents, which cannot completely inactivate it.
• Stable activity, ensuring long-term effective enzymatic effects.
• Exhibits antibacterial properties with a broad antibacterial spectrum.
• Possesses effective preservative qualities, aiding in the protection of experimental samples.

Operating Procedure (For reference only)

Follow specific experimental requirements. Generally, add this reagent at a volume ratio of bacterial suspension to lysozyme solution ranging from 6:1 to 10:1.

Precautions

• If using small volumes each time, it is advisable to aliquot the reagent appropriately to avoid repeated freeze-thaw cycles that may affect activity.
• To ensure the safety and health of experimenters, please wear a lab coat and disposable gloves during operation.
• Use the reagent promptly after opening to prevent a decrease in activity that could affect subsequent experimental results.

Specifications

Synonyms

Muramidase Solution

Specifications & Purity

BioReagent, Suitable for molecular biology, 10 mg/mL

Stability And Storage

Store at -20℃ long term (12 months). Avoid freeze/thaw cycle. Upon receipt,  it is recommended to aliquot.

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

BioReagent, Suitable for molecular biology

Names and Identifiers

• PH: 8.0