Magnetic adsorbent - Matrix:SiO2,Surface group:-C18,Size:0.1-1um,unit:5mg/ml

Cat. No.: M121905

GRADE & PURITY Matrix:SiO2,Surface group:-C18,Size:0.1-1um,unit:5mg/ml

Storage

Room temperature

Shipped In

Normal

Specifications

Specifications & Purity

Matrix:SiO2,Surface group:-C18,Size:0.1-1um,unit:5mg/ml

Storage

Room temperature

Shipped In

Normal

Application Protocols

No item-specific, validated protocols are provided for this SKU. The following general workflow outlines use of magnetic adsorbents in batch adsorption/separation (literature/practice):

• Preparation: Resuspend the adsorbent in the intended binding medium. If supplied dry, wet with a small volume of solvent, then dilute to working concentration. Sonicate briefly to disperse if needed.
• Equilibration: Wash 2–3× with binding buffer/solvent to equilibrate the surface.
• Binding: Add sample to the equilibrated adsorbent at a predetermined solid-to-liquid ratio. Mix gently for 1–15 min (optimize by analyte and surface chemistry).
• Separation: Place the vessel on a suitable magnet until the supernatant clears; carefully decant or aspirate.
• Wash: Add wash solution to remove non-specific binders; repeat as necessary.
• Elution/Release: Apply an eluent that disrupts the binding interaction (pH shift, high salt, competitive ligand, or increased organic content). Collect eluate after magnetic separation.
• Regeneration/Storage: If reuse is intended, rinse thoroughly with storage medium compatible with the coating; avoid conditions known to degrade the matrix.

For automation or high-throughput applications, select magnets and mixing compatible with microplates or robotic platforms. Validate recovery, carryover, and capacity for each new system.

Biological Roles

Item-specific biological roles or activities are not applicable. This product is a materials-based adsorbent for laboratory use and has no inherent biological role.

General context (literature/practice):

• Magnetic adsorbents, especially iron oxide-based, are widely used as tools in biochemical workflows (e.g., nucleic acid cleanup, protein purification via affinity ligands). Any observed selectivity arises from engineered surface chemistries rather than intrinsic biological activity.
• Iron oxide cores are often considered biocompatible in research contexts; however, biological interactions (protein corona formation, oxidative stress potential) depend strongly on surface coating and size. Such effects are application- and formulation-specific and are not claims for this item.
• For research use only: Not for use in diagnostic procedures or clinical applications.

Buffer Applications

Item-specific buffer compatibility and recommended formulations are not provided. The following are general buffer guidelines for magnetic adsorbent workflows (literature/practice):

• Binding buffers: Tailor to the interaction type.
  • Ion exchange: Low ionic strength; pH adjusted to charge the ligand opposite to the analyte.
  • Hydrophobic interaction: Moderate salt (e.g., 0.5–2 M ammonium sulfate) to promote hydrophobic binding.
  • Metal affinity (e.g., Ni–NTA): 20–50 mM phosphate or Tris, 300 mM NaCl; low imidazole for binding (5–20 mM).
  • Silica-like for nucleic acids: High salt plus alcohol (e.g., guanidinium + ethanol) for binding; water/low-salt for elution.
• Wash buffers: Increase ionic strength or add mild organic content (5–20% ethanol or acetonitrile) to remove nonspecific binders without eluting targets.
• Elution buffers: Compete (e.g., 250–500 mM imidazole for His-tag proteins), shift pH, lower salt, or raise organic content (50–100% MeCN for small molecules; water for DNA/RNA).
• Additives: 0.01–0.1% nonionic surfactants can improve wetting; avoid detergents that denature targets unless intended. Chelators can strip metal-affinity surfaces—use only when eluting metal-bound analytes.
• Practical notes: Filter buffers to remove particulates; match buffer pH and conductivity to validated protocols for your specific surface chemistry. Verify that buffers do not exceed the stability window of the adsorbent’s shell/coating.

Green Alternatives

While a magnetic adsorbent is itself a separation medium rather than a solvent or reagent, its use can contribute to greener workflows by reducing energy, time, and waste associated with phase separation and filtration. Consider the following sustainability aspects (general/literature):

Benefits of magnetic separation vs. conventional approaches:

• Eliminates or reduces centrifugation and vacuum filtration, cutting electricity use and equipment wear.
• Minimizes use of filter aids and disposable cartridges; reduces solid waste.
• Enables operation at ambient temperature and pressure, often shortening cycle times and solvent exposure.

Tradeoffs and considerations:

• Upstream synthesis of magnetic composites may involve energy- and solvent-intensive steps; selecting products with documented greener manufacturing or recycled matrices can improve footprint.
• Iron oxide cores are generally abundant and low-toxicity, but polymeric or fluorinated coatings can raise EHS concerns—prefer recyclable or bio-based coatings when available.
• Reusability/regeneration extends lifetime and reduces waste; verify that capacity remains within acceptable limits over cycles.

Comparison snapshot (general):

• Magnetic adsorbent vs. centrifuge-based separations: Similar selectivity if chemistry is equivalent, but magnetic offers simpler scaling and parallelization; centrifugation may achieve faster phase clarification at very small particle sizes (<50 nm) or very viscous media.
• Magnetic adsorbent vs. packed SPE cartridges: Magnetic formats reduce plastic hardware, allow direct processing in original vessels; cartridges offer higher pressure-driven flow for large volumes.

Pharmaceutical Uses

Item-specific pharmacopeial status or excipient use is not provided. This product is supplied for research use only and is not intended for use in humans or for diagnostic purposes.

General roles in pharmaceutical R&D and manufacturing research (literature/practice):

• Process development tools: Magnetic adsorbents functionalized for ion exchange or affinity can be used in high-throughput screening of purification conditions for biologics and peptides.
• Impurity scavenging: Removal of residual catalysts, reagents, and color bodies from drug-substance intermediates in medicinal chemistry workflows.
• Sample preparation: Cleanup of biological matrices prior to LC–MS analysis (e.g., phospholipid removal, protein precipitation aids with magnetic clarification).
• Catalyst supports: Magnetic recovery of supported catalysts for route scouting and sustainability assessments.

Regulatory note: Any use in GMP manufacturing would require thorough qualification of raw materials, extractables/leachables studies, and validation of adsorbent removal. Such compliance data are not provided for this item.

Physical Properties

Item-specific physicochemical specifications are not provided for this SKU.

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Particle size / distribution: Not specified for this item; refer to CoA/Spec Sheet.
• Surface area / porosity: Not specified for this item; refer to CoA/Spec Sheet.
• Bulk density / true density: Not specified for this item; refer to CoA/Spec Sheet.
• Magnetic properties (e.g., saturation magnetization, coercivity): Not specified for this item; refer to CoA/Spec Sheet.
• Solvent/water compatibility: Not specified for this item; refer to CoA/Spec Sheet.
• pH stability window: Not specified for this item; refer to CoA/Spec Sheet.

Literature/general values and considerations (not item-specific):

• Common magnetic cores (magnetite Fe3O4 or maghemite γ-Fe2O3) exhibit superparamagnetism at nanoscale, enabling rapid, reversible magnetic capture without remanence (literature).
• Typical specific surface areas for porous magnetic adsorbents can range from tens to several hundred m2/g depending on matrix (carbon, silica, MOF/COF composites) (literature).
• Dispersibility is strongly affected by ionic strength and surface functionalization; mild sonication often restores dispersion (practice note).

Quality & Grades

Item-specific grade, purity, and stabilizer information are not provided for this SKU.

• Grade/Purity: Not specified for this item; refer to CoA/Spec Sheet.

General quality considerations for magnetic adsorbents (literature/practice):

• Key QC metrics typically include particle size/distribution (DLS, laser diffraction, SEM/TEM), surface area/porosity (BET/BJH), surface functional group density (titration, XPS, TGA), magnetic response (VSM), and leachables (ICP-OES for Fe and other metals).
• Low extractables and controlled ligand loading are important for reproducible adsorption capacity and low background in analytical workflows (e.g., SPE, proteomics sample prep).
• For chromatography/sample preparation, low organic residue and low UV background are preferred; some products are washed and conditioned to minimize fines and residual monomers.
• Stabilizers/dispersion aids (if any) may be present in aqueous slurries to prevent aggregation; these can influence binding and may require pre-washing. Presence/absence is item-specific—verify via CoA.
• Batch-to-batch documentation: Request CoA including adsorption capacity against a reference analyte, recommended pH window, and magnetization (emu/g) when method validation demands traceability.

Reaction & Applications

Item-specific application notes are not provided. The following summarizes common laboratory uses of magnetic adsorbents (literature/practice) and practical considerations.

Representative applications:

• Solid-phase extraction (SPE) and cleanup: Rapid capture and wash of analytes (e.g., peptides, nucleic acids, small molecules) with magnetic separation to remove matrix interferences.
• Scavenging in synthesis: Post-reaction removal of excess reagents, catalysts (e.g., Pd, Cu), or byproducts using functionalized magnetic scavengers; simplifies workup by magnet instead of filtration.
• Heterogeneous catalysis: Magnetic supports for catalysts enable facile recovery/reuse; support surface area and stability control performance.
• Environmental remediation research: Adsorption of dyes, heavy metals, and organics from water; fast magnetic retrieval reduces secondary waste.
• Affinity capture: When bearing specific ligands (e.g., streptavidin, Ni2+-NTA, antibodies), they enable selective biomolecule isolation.

Practical tips (general):

• Equilibration: Pre-wet and equilibrate in binding medium to ensure full activation of surface sites.
• Kinetics: Mixing/agitation is key—end-over-end or gentle vortexing improves mass transfer. Contact times often range from 1–30 min depending on target and particle size (literature).
• Loading: Avoid overloading; determine breakthrough curves where quantitative recovery is needed.
• Magnetic separation: Choose a magnet matching tube/plate geometry; allow sufficient clearing time but avoid prolonged exposure that compacts beds and slows resuspension.
• Regeneration: Many adsorbents tolerate sequential wash with strong eluents followed by reconditioning; check stability limits and monitor for loss of capacity.

Reaction Conditions

No item-specific conditions are provided. General operating conditions for adsorption and magnetic separation (literature/practice):

• Medium: Aqueous buffers or organic/aqueous mixtures depending on target and surface chemistry. Degassing is typically unnecessary unless oxygen-sensitive targets are present.
• Temperature: Ambient (20–25°C) is common; adsorption kinetics may improve modestly with warming (≤40°C) if compatible with the surface and analyte.
• pH: Tune to favor the intended interaction (e.g., opposite charge for ion exchange). Typical stability windows for silica-like coatings are pH ~2–8; polymeric coatings may tolerate wider ranges—confirm for the specific product.
• Contact time: 1–30 minutes with gentle end-over-end mixing or vortexing; smaller particles generally bind faster due to higher surface area.
• Solid-to-liquid ratio: Often 0.5–50 mg/mL depending on capacity and analyte concentration; optimize to avoid overloading and aggregation.
• Separation: Use an external magnet matched to vessel geometry; clearing times from seconds to a few minutes depending on particle size and viscosity.
• Regeneration: Wash with strong eluent (e.g., high salt, pH shift, or organic solvent), then re-equilibrate to binding conditions. Monitor for any visible degradation or capacity loss.

Expected outcomes (literature examples): Efficient removal of ppm–percent-level contaminants or quantitative capture of targets is feasible with properly matched surface chemistry and conditions; yields and capacities are system-specific and should be empirically determined.

Safety & Handling

GHS and hazard information for this specific item are not provided. Always consult the product SDS for authoritative safety information.

Item-specific hazard data:

• Signal Word: Not specified for this item; refer to SDS.
• Hazard Statements / Pictograms / Classification: Not specified for this item; refer to SDS.

General laboratory safety guidance for magnetic adsorbents (literature/practice):

• PPE: Use lab coat, safety glasses, and appropriate gloves. Avoid inhalation of dusts or aerosols; handle powders in a fume hood or with local exhaust.
• Physical hazards: Fine magnetic powders can be attracted to strong magnets rapidly—use caution to avoid pinch hazards and splashing of slurries.
• Chemical compatibility: Strong acids or bases and oxidants/reductants may dissolve or transform iron oxide cores and/or degrade organic coatings; chelators can strip surface metal sites. Confirm compatibility with your surface chemistry.
• Reactivity/incompatibilities: Avoid contact with strong oxidizers (for carbonaceous matrices) and with fluoride- or HF-containing media for silica shells (general caution). Do not autoclave unless validated for the specific material.
• Handling tips: Minimize prolonged exposure to air and moisture if the surface is prone to oxidation or hydrolysis. Disperse gently (vortex/sonication) to avoid attrition.
• First aid (overview): If inhaled—move to fresh air. Skin/eye contact—rinse with water for several minutes; remove contaminated clothing. If ingested—rinse mouth. Seek medical attention as needed. Defer to SDS for details.
• Waste: Collect spent adsorbent and any adsorbed hazardous substances as hazardous waste per institutional and local regulations.

Solvent Selection

Item-specific solvent compatibility is not provided; consult the CoA/SDS for definitive guidance. The following are general considerations for magnetic adsorbents used in separations and scavenging.

• Polarity and miscibility: Magnetic adsorbents are typically used as aqueous or mixed aqueous–organic slurries. Solvent choice depends on matrix and surface chemistry (silica-, polymer-, or carbon-based). Protic solvents aid hydrogen-bonding interactions; aprotic polar solvents (e.g., MeCN, DMSO) can modulate polarity during elution.
• Typical systems (literature/practice):
  • Silica-like surfaces: water, buffers (pH 2–8), alcohols; avoid strong bases and HF/fluoride.
  • Polymeric surfaces: broad solvent compatibility including water, alcohols, acetonitrile, some chlorinated solvents; avoid strong oxidizers.
  • Carbonaceous surfaces: good compatibility with water/organics; avoid strong oxidizing acids.
• Elution strategy: Increase ionic strength, adjust pH to disrupt electrostatic binding, add competitive ligands (e.g., imidazole, EDTA for metal-affinity surfaces), or increase organic content to weaken hydrophobic interactions.
• Comparison (general):
  • Magnetic adsorbent vs. conventional filtration/centrifugation media: Magnetic enables rapid phase separation without vacuum/centrifuge, reducing shear and equipment burden; however, viscosity and high solids content can slow capture, and repeated wash–capture cycles may be needed.
• Practical tips: Pre-equilibrate in binding solvent; degas if using in degassing-sensitive assays; verify that the magnet strength and geometry match the particle size to achieve rapid clearing.

Storage & Reconstitution

Item-specific details provided:

• Storage Conditions: Room temperature
• Shipped In: Normal

Additional item-specific details not provided:

• Appearance/format (dry powder vs. slurry), storage medium, and stabilizers: Not specified for this item; refer to CoA/Spec Sheet.

General guidance (literature/practice):

• If supplied as a dry solid: Store tightly closed in a dry environment away from corrosive vapors. Before use, wet with a compatible solvent (e.g., water or buffer for hydrophilic surfaces; alcohol for hydrophobic surfaces) and gently sonicate to disperse.
• If supplied as an aqueous slurry: Store at recommended temperature (RT for many polymer/silica-coated materials) and avoid freezing unless validated, as freeze–thaw can cause aggregation or shell damage. Mix to homogeneity before aliquoting (end-over-end or vortex). If bioburden control is needed, use sterile technique or add a compatible preservative validated for your application.
• Avoid prolonged exposure to strong acids/bases, oxidants, or chelators unless required for regeneration and within the material’s stability window.
• Do not store in direct contact with strong permanent magnets; instead, keep in standard lab storage to prevent compaction.
• For long-term storage or reuse, document the number of use cycles and any observed changes in capacity or dispersibility.

Always consult the SDS/CoA for definitive storage and reconstitution instructions specific to this product.

Structure & Identity

Item-specific identifiers are not provided for this product. Where precise composition or surface chemistry is required, consult the CoA/Spec Sheet.

• SKU: M121905
• Product Name: Magnetic adsorbent
• CAS: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular Formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular Weight: Not specified for this item; refer to CoA/Spec Sheet.
• SMILES / InChI / InChIKey: Not specified for this item; refer to CoA/Spec Sheet.

General materials description (literature/general knowledge):

• Magnetic adsorbents are typically composite particulates that incorporate a magnetic core (commonly iron oxides such as magnetite or maghemite) embedded in or coated by an inorganic (e.g., silica) or organic (e.g., polymer, carbon) matrix.
• Structural features usually include: a magnetic core enabling rapid separation under an external magnet; a shell/coating that confers chemical stability and dispersibility; and optional surface functional groups (e.g., –OH, –COOH, –NH2, chelators, ion-exchange moieties) for selective adsorption.
• Morphology ranges from nano- to micro-scale spheres or irregular granules; core–shell, yolk–shell, and porous architectures are common (literature). 2D representation would depict a core (magnetic phase) surrounded by a functionalized shell exposing adsorption groups to the medium.

Synthetic Utility

Item-specific functional groups and loading are not provided. The following outlines typical synthetic utility of magnetic adsorbents (literature/practice):

• Scavenging functional groups: Surfaces bearing nucleophiles (e.g., primary/secondary amines, thiols), electrophiles (e.g., isocyanates, sulfonyl chlorides on polymer shells), ion-exchange sites, or chelating ligands can quench or capture complementary reagents and metal catalysts (Pd, Cu, Ru) from reaction mixtures.
• Workup simplification: After dosing the scavenger, apply a magnet to clear the solution, decant, and proceed without filtration—useful for moisture/air-sensitive streams when coupled with inert handling.
• Supported reagents: Magnetic particles can carry oxidants, bases, or coupling reagents to facilitate heterogeneous transformations with easy recovery.
• Reusability: Robust shells (e.g., crosslinked polymers, silica) allow multiple cycles when regeneration conditions are compatible with both the shell and the captured species.
• Retrosynthetic value: Enables late-stage diversification workflows where rapid purification is required for parallel synthesis; can be paired with catch-and-release strategies for amines, acids, and alcohols.

Practical pointers:

• Determine stoichiometry by small-scale titration against a known substrate to estimate capacity.
• Beware of co-adsorption of desired products on highly hydrophobic or charged surfaces—optimize solvent polarity and ionic strength to minimize losses.

Target Specificity

Not applicable. This product is a generic magnetic adsorbent and is not an antibody or biological probe. No antigen/epitope, clone, isotype, or species reactivity information applies to this item.