Magnetic adsorbent - Matrix:SiO2,Surface group:-C18,Size:1-2um,unit:10mg/ml

Cat. No.: M121906

GRADE & PURITY Matrix:SiO2,Surface group:-C18,Size:1-2um,unit:10mg/ml

Storage

Room temperature

Shipped In

Normal

Specifications

Specifications & Purity

Matrix:SiO2,Surface group:-C18,Size:1-2um,unit:10mg/ml

Storage

Room temperature

Shipped In

Normal

Application Protocols

Item-specific validated protocols: Not specified for this item; refer to CoA/Spec Sheet. The following is a general protocol for batch adsorption using magnetic adsorbents (literature/practice; not item-specific):

General batch adsorption workflow:

1. Equilibration

• Resuspend the magnetic adsorbent in the working buffer/solvent. If supplied dry, wet with a miscible solvent (e.g., ethanol or water), then exchange into the target medium.
• Equilibrate with 5–10 volumes of buffer to stabilize pH/ionic strength and remove fines.

2. Binding

• Add the conditioned adsorbent to the sample (typical 1–20 mg/mL; adjust to impurity load).
• Mix gently but thoroughly (end-over-end, overhead stir) for 5–30 minutes, maintaining temperature and pH.

3. Magnetic separation

• Place a neodymium magnet against the vessel; allow the supernatant to clear (30 s–5 min). Decant or aspirate the supernatant.

4. Washing

• Add wash medium (same buffer or with adjusted ionic strength/organic content), resuspend, mix 1–5 minutes, magnetically separate, and decant. Repeat 1–3 times.

5. Elution/regeneration (if desired)

• Elute bound species with appropriate agent (pH shift, salt, chaotrope, or organic modifier). Collect and neutralize if needed.
• Regenerate by cycling between wash and storage buffers; confirm capacity retention.

6. Storage

• Exchange into storage medium recommended for the material (see Storage & Reconstitution). Avoid microbial growth (biocide or refrigeration as appropriate).

Notes:

• Record mass balance and adsorption isotherms to optimize dose and contact time.
• For viscous or organic-rich matrices, extend mixing/separation times and verify coating stability.
• Always confirm compatibility and capacity for SKU M121906 from its CoA before adopting the protocol.

Biological Roles

Item-specific biological role: Not applicable; this is a materials/adsorbent product for laboratory use. No inherent biological function is specified for SKU M121906.

General notes (literature; not item-specific):

• Magnetic adsorbents themselves do not possess intrinsic biological roles; however, when functionalized appropriately, they are widely used as tools in biochemistry and molecular biology for:
  • Nucleic acid purification (silica-like surfaces binding DNA/RNA under chaotropes or alcohol).
  • Protein/peptide capture via ion exchange, hydrophobic interaction, immobilized metal affinity (IMAC), or affinity tags (e.g., Ni–NTA for His-tags; streptavidin–biotin systems).
  • Cell separation when conjugated to antibodies/ligands targeting surface markers (note: such targeted products have defined biological specificities not described here).
• Biocompatibility depends on the surface chemistry: Dextran/PEG coatings often reduce nonspecific binding; amine-rich surfaces may interact with cell membranes and proteins more strongly.
• Endotoxin/bioburden levels can be critical for sensitive bioassays; specialized “bioprocess” grades control these metrics. For general research-grade adsorbents, users should assess suitability for their assay context.

Caution: Do not infer any diagnostic, therapeutic, or clinical uses from the product name. This product is supplied strictly for research use only. For experiments involving cells or biomolecules, confirm surface chemistry, leachables, and compatibility from the CoA/SDS and perform pilot tests.

Buffer Applications

Item-specific buffer recommendations: Not specified for this item; refer to CoA/Spec Sheet.

General guidance for using magnetic adsorbents in buffered systems (literature/practice; not item-specific):

• Buffer choice is dictated by the adsorption mechanism:
  • Electrostatic capture: Choose buffers that maintain pH relative to the adsorbent’s pHpzc and analyte pKa. Avoid multidentate chelators if iron leaching is a risk.
  • Affinity/metal-chelate systems: Use buffers without competing ligands (e.g., avoid imidazole unless eluting from Ni-based IMAC; avoid phosphate for calcium-based interactions where applicable).
• Common buffers and ranges (typical):
  • Acetate (pH 3.5–5.5), MES (pH 5.5–6.7), MOPS (pH 6.5–7.9), HEPES (pH 6.8–8.2), Tris (pH 7.2–9.0), carbonate/bicarbonate (pH 9–10). Select ionic strength (10–500 mM) to tune binding.
• Additives:
  • 0.01–0.1% non-ionic surfactant (e.g., Tween 20) can reduce nonspecific adsorption in protein workflows; confirm compatibility with the coating.
  • 5–20% ethanol or isopropanol may aid hydrophobic desorption; ensure polymer shells tolerate solvents.
• Practical tips:
  • Equilibrate adsorbent with 5–10 bed volumes of buffer prior to use to stabilize surface charge and remove fines.
  • For batch capture, maintain a solid loading (e.g., 1–20 mg/mL suspension, literature) and gentle mixing to avoid bead attrition.
  • Record pH drift on initial contact; oxide surfaces can shift pH slightly in low-buffer-capacity systems.

Because the surface chemistry of SKU M121906 is not specified, verify buffer compatibility and performance empirically at small scale before process deployment.

Green Alternatives

Perspective: Magnetic adsorbents can reduce resource use by enabling fast, equipment-light separations and facile material recovery. Below is a balanced comparison with common alternatives (general literature; not item-specific).

Sustainability considerations:

• Reduced energy/waste: Magnetic decantation can replace centrifugation/filtration, lowering energy and consumables (filters) and minimizing product loss.
• Reusability: Magnetic supports are often regenerable across multiple cycles, reducing sorbent consumption.
• Process intensification: Faster phase disengagement can shorten cycle time and footprint.

Comparison (general):

| Option | Strengths | Tradeoffs | |---|---|---| | Magnetic adsorbent | Rapid separations; easy recovery/reuse; scalable batch processing | Potential iron leaching without robust shells; magnet capital; sensitivity to chelators/oxidants | | Activated carbon | High surface area; broad-spectrum organics removal | Difficult regeneration; filtration needed; dust; variable selectivity | | Silica/oxide beads (nonmagnetic) | Robust; tunable chemistry; column-compatible | Requires filtration or centrifugation; fines handling | | Ion-exchange resins | High capacity/selectivity for ions | Swelling; regeneration brines; column infrastructure | | Liquid–liquid extraction | High throughput | Emulsions; solvent inventory; disposal burden |

Greener choices within magnetic adsorbents:

• Prefer silica or carbon shells over halogenated polymers; avoid PFAS-based surfactants.
• Use water/ethanol as conditioning/wash solvents where feasible.
• Validate mild regenerants (e.g., pH shifts, salt) before strong acids/bases.
• Track capacity retention and iron leach to maximize lifetime and minimize waste.

For SKU M121906, specific materials and eco-metrics are not provided—verify with the CoA/SDS and your internal sustainability framework.

Pharmaceutical Uses

Item-specific pharmacopeial status or excipient role: Not specified for this item; refer to CoA/Spec Sheet. This product is for research use only and is not intended for clinical or diagnostic applications.

General context (materials/process development; not item-specific):

• Magnetic adsorbents may be evaluated as process aids in pharmaceutical R&D and manufacturing development, for example:
  • Rapid clarification and impurity scavenging during route scouting and scale-up.
  • Recovery of homogeneous/heterogeneous catalysts from reaction mixtures.
  • Capture and release of biomolecules in upstream/downstream development studies (non-GMP unless specified).
• Considerations for pharma workflows:
  • Extractables/leachables: Assess iron leach and coating components under process conditions.
  • Residuals control: Validate removal of the adsorbent and any fines to meet specification.
  • Regeneration and reuse: Establish cleaning protocols that maintain capacity and do not introduce cross-contamination.
  • Regulatory alignment: For any move toward GMP use, materials need defined composition, tight impurity control, and change control from the supplier.
• Analytical support:
  • Document adsorption isotherms, breakthrough behavior, and desorption profiles for target impurities.
  • Verify no interference with critical quality attributes (e.g., assay interferences, particulate counts) in the formulated context.

Note: No pharmacopeial monograph or specific formulation role is claimed for SKU M121906 based on the provided information.

Physical Properties

Item-specific physical properties: Not specified for this item; refer to CoA/Spec Sheet.

Typical properties for magnetic adsorbents (literature/general; not item-specific):

• Core density (Fe3O4): ~5.2 g/cm³ (literature). Apparent bulk density depends on granulation/porosity.
• Magnetic parameters: Saturation magnetization (Ms) for magnetite nanoparticles commonly 50–80 emu/g (literature), reduced from bulk due to surface effects and coatings.
• Thermal behavior: Iron oxides are thermally robust; organic/polymer coatings may limit operating temperature to 60–120 °C (literature) depending on chemistry.
• Surface area: Wide range; mesoporous/shell-coated variants can exhibit 50–400 m²/g (BET, literature). Granulated beads show lower external area but improved flow.
• Particle size: Primary nanoparticles often 10–50 nm (TEM, literature). Beads/composites can be 1–200 µm (laser diffraction, literature) for batch/column use.
• Surface charge (pHpzc): Magnetite point of zero charge often ~6.5–7.5 (literature); functional shells shift pHpzc markedly (e.g., amines raise, carboxyls lower).
• Solvent compatibility: Typically compatible with water, many alcohols, and buffered media. Stability in strong acids/bases, chelating agents, or oxidants depends on coating/shell (literature guidance only).

Reporting note for this item: Specific values such as particle size distribution, BET surface area, functional group loading, moisture content, and magnetic response time are not provided here—consult the CoA/Spec Sheet for definitive specifications.

Quality & Grades

Item-specific grade/purity for SKU M121906: Not specified for this item; refer to CoA/Spec Sheet.

How quality is typically defined for magnetic adsorbents (general guidance; not item-specific):

• Composition and identity: Confirmation of iron-oxide phase (XRD), absence of unwanted crystalline impurities, and verification of shell/coating chemistry (FTIR, XPS, TGA).
• Particle metrics: Particle size distribution (TEM/DLS/laser diffraction), BET surface area/porosity, sphericity for bead formats, and sedimentation behavior.
• Magnetic performance: Saturation magnetization (Ms), remanence (Mr), and magnetic separation time in standard geometry (e.g., 1 mL water, 1 T magnet, 1 cm distance).
• Functional performance: Adsorption capacity (qmax, mg/g) for a model solute, equilibrium constants (Langmuir/Freundlich fits), and selectivity in the presence of competing ions/solutes.
• Leachables/Extractables: Iron leaching under acidic/neutral/basic conditions, residual monomers/solvents below method thresholds (GC/MS, LC/MS), and ash content.
• Cleanliness for analytical work: Low background metal contaminants where relevant (ICP-MS screens), and minimal UV/fluorescence background if used in analytical separations.

Grade meaning (examples, literature):

• Research grade: Suitable for method development, general lab separations.
• Bioprocess/“mag-bead” grade: Tighter control of endotoxin/bioburden, narrow size distribution, defined ligand density.

For SKU M121906, consult the CoA for exact grade, QC tests performed, and acceptance criteria. Stabilizers, moisture content, and recommended pretreatments are not specified for this item.

Reaction & Applications

Item-specific applications listed by manufacturer: Not specified for this item; the catalog name indicates use as a magnetic adsorbent for separations. For research use only.

General applications of magnetic adsorbents (literature/practice; not item-specific):

• Rapid solid–liquid separations: Enable magnetic decantation instead of filtration/centrifugation, minimizing solids loss and equipment fouling.
• Pollutant capture: Removal of heavy metals, oxyanions (e.g., As(V), Cr(VI), phosphate), and dyes from water; regeneration by pH or ligand exchange.
• Organic synthesis support: Scavenging residual catalysts (Pd, Cu), acids/bases, or reactive intermediates; simplifies workups via magnet-assisted removal.
• Biochemical workflows: Capture of nucleic acids, proteins, or glycoproteins with appropriately functionalized surfaces (e.g., carboxyl/amine/IMAC/streptavidin), followed by magnet-based washes and elution.
• Catalysis: As supports for heterogeneous catalysts (e.g., Fe3O4@SiO2–Pd), allowing magnetic recovery and reuse.
• Sample prep and SPE: Preconcentration from complex matrices; compatible with batch mode or packed in low-field cartridges.

Practical tips:

• Pre-condition in the working medium to fully wet and equilibrate the surface; remove fines if necessary to reduce background.
• For electrostatic adsorption, tune pH relative to analyte pKa and adsorbent pHpzc; monitor conductivity.
• Use adequate mixing (end-over-end, overhead stirring) to minimize external film resistance; typical contact times 1–30 min (literature) depending on size and diffusion.
• Separate with a strong neodymium magnet; allow additional time for viscous or organic-rich media.
• Regenerate using appropriate eluents (e.g., acid/base, salt gradients, organic modifiers) and verify capacity retention over cycles.

Document lot-specific capacities and breakthrough curves using your target solutes to support method robustness.

Reaction Conditions

Item-specific operating windows for SKU M121906 (pH, solvent, temperature, capacity): Not specified for this item; refer to CoA/Spec Sheet.

General operational conditions for magnetic adsorption and related uses (literature/practice; not item-specific):

• Temperature: Ambient (20–25 °C) is typical for adsorption; elevated temperatures (30–60 °C) can speed kinetics but may reduce equilibrium capacity for exothermic adsorption.
• pH: Tune 2–10 depending on target analyte and surface chemistry; avoid extremes if polymeric coatings are present. For metal oxyanion capture, acidic to neutral ranges often favored; for cationic dye capture, basic pH can help on acidic surfaces.
• Contact time: 1–30 minutes with vigorous mixing is common for nanoparticles; 10–60 minutes for larger beads or viscous matrices (literature).
• Solid loading: 0.5–50 mg/mL suspension depending on capacity and impurity burden; start small and titrate upward.
• Separation: Neodymium magnets (N42–N52) placed externally; allow 30 s–5 min for complete clearing depending on path length and viscosity.
• Regeneration: Acid/base elution (e.g., 0.1–1 M HCl/NaOH), salt gradients (0.5–2 M NaCl), or organic modifiers (20–80% alcohol or MeCN). Rinse thoroughly between cycles.
• Compatibility checks: Screen for iron leach in acidic media and for coating integrity in oxidizing or strongly chelating environments.

Reporting and scale-up tips:

• Establish mass transfer regime (shake speed, impeller type) to ensure reproducibility.
• Fit isotherms (Langmuir/Freundlich) to extract qmax and b; conduct kinetics (pseudo-first/second order) for cycle time design.
• For catalytic applications, document turnover numbers and magnet-assisted recovery efficiency per cycle.

All values above are general literature guidance and not specifications for this item.

Safety & Handling

Item-specific hazard classification for SKU M121906:

• Signal word, H-statements, GHS classification, pictograms: Not specified for this item; refer to SDS.

General safety guidance for magnetic adsorbents (literature/practice; not item-specific):

• Potential hazards: Fine powders may pose dust inhalation and mechanical irritation risks. Strong local magnetic fields can attract ferromagnetic tools and pinch hazards. Some surface ligands or residual monomers/solvents may be irritants.
• PPE: Use lab coat, safety glasses, and appropriate gloves (nitrile commonly suitable). Employ dust control (handle in a fume hood) if powdery. For slurries, avoid aerosol formation.
• Handling: Keep containers closed to prevent moisture uptake or contamination. Use nonmagnetic tools when practical. Apply magnets externally to vessels; avoid direct contact of magnets with bulk solids to prevent compaction.
• Incompatibilities (depend on surface/ core): Strong acids/bases and oxidants can degrade organic coatings; strong chelators (e.g., EDTA) may mobilize iron; hypochlorite can attack polymeric shells. Avoid prolonged exposure to corrosive media unless validated.
• First aid (overview; defer to SDS): If inhaled, move to fresh air; if on skin/eyes, rinse with water; if ingested, rinse mouth—seek medical advice in all cases.
• Spill response: For powders, avoid dusting; dampen and collect mechanically. For slurries, use absorbents compatible with the solvent matrix. Collect with non-sparking, nonmagnetic tools.
• Waste: Dispose according to local regulations for inorganic/ polymeric solid waste; account for any adsorbed hazardous species on the solid.

Always consult the product-specific SDS for authoritative safety information.

Solvent Selection

Applicability: As a solid magnetic adsorbent, “solvent selection” relates to dispersing media for adsorption and magnetic separation rather than the compound dissolving.

Item-specific compatibility: Not specified for this item; refer to CoA/Spec Sheet.

General guidance (literature/practice; not item-specific):

• Polarity window:
  • Water and aqueous buffers (pH 2–10 typical) are commonly compatible, especially for oxide or silica-shelled materials.
  • Polar organics (methanol, ethanol, isopropanol, acetonitrile) often acceptable for washing/conditioning; check for swelling/softening if polymer-coated.
  • Nonpolar solvents (hexane, toluene, DCM) compatibility varies with shell and binder; test a small aliquot first.
• Ionic strength and pH:
  • Electrostatic adsorption is highly pH-dependent relative to the adsorbent’s pHpzc/functional group pKa.
  • Moderate salt (0.1–1 M) can screen charges (affecting capacity) or promote salting-out for hydrophobic capture.
• Additives:
  • Avoid strong chelators (EDTA, citrate) when iron leaching is a concern unless a protective shell is present.
  • Surfactants can modify wettability and non-specific binding; validate concentrations carefully.

When to choose aqueous vs organic media:

• Aqueous buffers for biomolecules, metal ions, and ionic dyes.
• Alcohols/MeCN for small organic contaminants and to reduce viscosity for faster mass transfer.

Quick comparison (general; not item-specific):

• Water/buffer: safest, tunable pH/ionic strength, easy waste handling.
• Alcohols: rapid wetting/drying cycles, good for regeneration.
• Nonpolars: useful for hydrophobic desorption but may stress polymeric binders.

Always verify medium compatibility and adsorption performance with a small-scale test before scale-up.

Storage & Reconstitution

Item-specific instructions from Product Data:

• Storage conditions: Room temperature.
• Shipped in: Normal.
• Appearance, stabilizers, and exact storage medium: Not specified for this item; refer to CoA/Spec Sheet.

General storage guidance for magnetic adsorbents (literature/practice; not item-specific):

• Dry powders: Store tightly sealed, away from humidity and strong magnetic fields. Include a desiccant if moisture is detrimental to performance. Avoid repeated high-energy magnetic exposures that may cause agglomeration.
• Aqueous slurries: Store in a compatible container (nonmagnetic cap/closure preferred) at room temperature unless a biocide or refrigeration is specified. Gentle inversion before use to redisperse. For long-term storage, addition of 0.02–0.05% sodium azide or 20% ethanol is common in research settings—verify compatibility and safety policies.
• Organic slurries: Ensure solvent-tight containers; periodically check volume due to evaporation. Prevent exposure to oxidants/acids/bases that could degrade coatings.

Reconstitution/dispersion (if supplied dry; general):

• Wetting: Pre-wet with ethanol or isopropanol, then exchange to water/buffer to minimize trapped air; alternatively, wet directly with the working solvent.
• Dispersion: Vortex or overhead stir; brief bath sonication (1–5 min) can help break soft agglomerates. Avoid probe sonication unless coating is known to tolerate shear.
• Conditioning: Rinse with several bed volumes of the intended working medium to equilibrate pH/ionic strength and remove fines.

Always defer to the product-specific CoA/SDS for definitive storage medium, preservatives, and any temperature or light sensitivities for SKU M121906. For research use only.

Structure & Identity

Item-specific identifiers: Not specified for this item; refer to CoA/Spec Sheet.

• SKU: M121906
• Product name: Magnetic adsorbent (research-grade solid phase for magnetically assisted separations)
• CAS, CID, InChIKey, SMILES: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular formula and molecular weight: Not specified for this item; refer to CoA/Spec Sheet.

General description (literature/typical for magnetic adsorbents; not item-specific):

• Core materials commonly used include magnetite (Fe3O4) or maghemite (γ‑Fe2O3) nanoparticles that exhibit superparamagnetism at small sizes.
• Surface architectures often incorporate:
  • Inorganic shells (e.g., silica, carbon, titania) for chemical robustness and tunable porosity.
  • Polymeric coatings (e.g., dextran, PEG, polystyrene, PDMS, PEI) for colloidal stability and functionalization.
  • Functional groups (–COOH, –NH2, –SH, –OH, quaternary ammonium, phosphonate, imidazole, chelators) to impart adsorption selectivity.
• Structural features (2D description): A magnetic core–shell particle in which a high-contrast iron‑oxide core is surrounded by a thin shell or grafted polymer bearing adsorptive sites. Primary particles (10–100 nm, literature) may be aggregated/granulated into microbeads for easier handling.
• Magnetic behavior (literature): Often superparamagnetic (minimal remanence), enabling rapid capture with a magnet and redispersal when the field is removed.

Note: The specific composition, particle size, surface chemistry, and functional loading for SKU M121906 are not specified here and should be confirmed from the product’s CoA/Spec Sheet.

Synthetic Utility

Item-specific functional groups and loading: Not specified for this item; refer to CoA/Spec Sheet.

General synthetic utility of magnetic adsorbents (literature; not item-specific):

• Workup simplification: Use as scavengers for acids/bases, residual metals (e.g., Pd, Ru, Cu), halides, or reactive electrophiles/nucleophiles. Magnetic capture streamlines separation from reaction mixtures, particularly viscous or heterogeneous systems.
• Heterogeneous catalysis platform: Magnetic core–shells provide recoverable supports for Pd, Ni, Cu, Au nanoparticles, organocatalysts, or enzymes, enabling repetitive use and cleaner product profiles.
• Flow/batch flexibility: Granulated magnetic beads can be employed in batch adsorption or packed into cartridges for semi-continuous operation under a magnetic field gradient.
• Orthogonal selectivity: By choosing surface chemistry (amine, thiol, quaternary ammonium, sulfonic acid, chelating ligands), the adsorbent targets specific impurity classes without affecting desired products.
• Moisture/air tolerance: Oxide-based cores are robust; however, surface functionalities may require anhydrous/oxygen-limited handling depending on reactivity.

Practical insights:

• Pre-screen sorbent dose (e.g., 2–20 wt% vs crude mass, literature) to achieve target residuals.
• Validate compatibility with solvents from nonpolar to polar aprotic; confirm no adverse adsorption of product.
• Establish regeneration (acid/base wash, alcohol rinses, chelator if appropriate) and verify capacity over 3–10 cycles.
• For metal scavenging, monitor residual metal by ICP-MS; for ionic impurities, apply ion chromatography or titration.

For SKU M121906, confirm the surface functionality and capacity from the CoA before designing purification strategies.

Target Specificity

Not applicable. SKU M121906 is a generic magnetic adsorbent material and is not an antibody, affinity ligand, or biologic with defined antigen/epitope specificity.

• Item-specific target/epitope, clone, species reactivity, or isotype: Not specified for this item; refer to CoA/Spec Sheet if a specialized affinity version is supplied.

If this material is a functionalized magnetic adsorbent (e.g., IMAC, ion exchange, streptavidin), consult the lot-specific documentation for ligand density and binding capacity toward the intended target.