Malt Extract Broth - BioReagent

Cat. No.: M1528374

GRADE & PURITY BioReagent

Storage

Room temperature

Shipped In

Normal

Overview

Contains 1.5% malt extract powder, chloramphenicol-free, for sterility testing of acidic canned foods, and also for the cultivation of molds and yeasts (GB)

Specifications

Specifications & Purity

BioReagent

Storage

Room temperature

Shipped In

Normal

Grade

BioReagent

Product Line

Biological Products

Application Protocols

Item-specific tested applications and instructions: Not specified for this item; refer to CoA/Spec Sheet.

General/literature protocol for Malt Extract Broth (not item-specific; adjust per organism and method):

1. Preparation
   • Weigh the label-specified mass of dehydrated medium and add to the appropriate volume of purified water. Stir to dissolve. Avoid excessive heating before sterilization.
2. Sterilization
   • Autoclave at 121 C for ~15 min (standard cycle). Allow to cool to 45–50 C if adding heat-labile supplements (sterile-filtered).
3. Inoculation
   • Aseptically inoculate with the organism of interest. For yeasts, set shaking at 150–250 rpm in baffled flasks to enhance aeration; for molds, static culture may be suitable.
4. Incubation
   • Incubate at organism-appropriate temperature (often 22–30 C). Monitor growth by OD600 for yeasts or dry weight/colony counts as appropriate.
5. Quality checks
   • Verify sterility controls, pH, and growth-promotion using reference strains when method validation is required.

Always follow institutional biosafety procedures and this product’s CoA/label for exact preparation grams-per-liter and pH targets.

Biological Roles

Item type: Complex growth medium supporting fungal/yeast biology; not a biomolecule with intrinsic cellular function.

General/literature context (not item-specific):

• Nutritional profile: Malt extract provides fermentable sugars (e.g., maltose, glucose), amino nitrogen, minerals, and vitamins, supporting rapid proliferation of many yeasts (Saccharomyces, Candida spp.) and fungi (Aspergillus, Penicillium, etc.).
• Physiology supported: biomass accumulation, sporulation for certain molds, and production of primary metabolites under aerobic conditions.
• Selectivity: The mildly acidic nature of many MEB recipes disfavors many bacteria while favoring fungi/yeasts; however, MEB is typically considered non-selective within yeasts/molds unless further supplemented (literature/general).
• Experimental uses: studying carbon utilization, monitoring fermentation performance, preparing inocula for enzymology or metabolite profiling, and assessing stress responses (osmotic, pH) in industrial strains.

Note: Exact composition, pH, and performance criteria for this SKU are not specified here—consult the CoA/Spec Sheet and validate with growth-promotion testing for organisms relevant to your laboratory.

Buffer Applications

This product is a growth medium and is not intended as a buffering reagent. While many malt extract broth formulations exhibit mildly acidic pH after preparation, precise buffer capacity and composition are formulation-dependent.

• Item-specific buffer system and pKa details: Not specified for this item; refer to CoA/Spec Sheet.
• For controlled pH studies, use dedicated biological buffers (e.g., citrate, phosphate, MOPS) and verify compatibility with your target microorganisms (literature/general).

Green Alternatives

Perspective on environmental and operational footprint (literature/general):

• Dehydrated media reduce transportation weight/volume compared with ready-to-use liquids, lowering shipping-related emissions.
• Autoclave sterilization consumes energy; consider load consolidation and cycle optimization.

Comparison of common fungal/yeast media (literature/general; not item-specific):

• Malt Extract Broth (MEB): malt-derived nutrients; typically mildly acidic; broadly supports yeasts/molds.
• Potato Dextrose Broth (PDB): potato infusion and dextrose; strong sporulation for some fungi; slightly different nutrient spectrum.
• Sabouraud Dextrose Broth (SDB): peptone plus high dextrose; often more general fungal growth; pH commonly around 5.6.

Greener practice tips:

• Use reusable glassware and validated cleaning to reduce single-use plastics where biosafety permits.
• Prepare only the volume required; store aliquots appropriately to reduce waste.
• Consider energy-efficient autoclaves and schedule sterilizations to maximize chamber utilization.
• Dispose of spent media via validated decontamination to prevent environmental release of viable organisms.

Pharmaceutical Uses

No therapeutic or clinical claims. For research and quality-control contexts only.

General/literature applications in regulated environments (not item-specific):

• Microbiological environmental isolates: cultivation and maintenance of yeast/fungal strains used in method development and validation.
• Upstream screening: assessing growth characteristics of production or host strains in early research phases.
• Process hygiene or spoilage investigations in allied industries (food/beverage, nutraceutical) where yeasts/molds are of interest.

Regulatory note:

• Pharmacopeial status, compendial equivalence, and specific performance specifications for this SKU are not specified; consult your quality unit and the product’s CoA/SDS if compendial methods are required.

Physical Properties

Item-specific properties (from Product Data):

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Storage conditions: Room temperature (provided).
• Density, pH (at use concentration), moisture content, solubility: Not specified for this item; refer to CoA/Spec Sheet.

General/literature characteristics for Malt Extract Broth (not item-specific):

• Typical physical form: free-flowing, tan-to-light-brown dehydrated powder; readily soluble in water (literature/general).
• Typical working concentration in water: often prepared at a defined grams-per-liter per label; common ranges 20–50 g/L are reported across suppliers (literature/general; follow this product’s label/CoA for exact value).
• Typical prepared-broth pH (25 C): many MEB formulations target mildly acidic pH (≈5.2–5.6) to favor fungi/yeasts (literature/general; not item-specific).
• Osmolality, refractive index, viscosity: context-dependent on concentration; not standardized (literature/general).

Notes for use:

• Use purified water (e.g., ASTM Type II or better) for reproducibility.
• Avoid prolonged heating prior to sterilization to minimize Maillard reactions and sugar decomposition (literature/general).

Quality & Grades

• Item-specific grade: BioReagent (from Product Data).
  • Interpretation (general): BioReagent-grade materials are intended for biological and biochemical research workflows, emphasizing consistency and suitability for microbiology/molecular biology applications. Not for human or animal therapeutic use.
• Purity specifications, composition, and performance criteria for this specific lot: Not specified for this item; refer to CoA/Spec Sheet.
• Stabilizers/antimicrobials: Not specified for this item; refer to CoA/Spec Sheet.

What BioReagent means in practice (general/literature):

• Emphasis on low bioburden in dehydrated form and batch-to-batch reproducibility of growth performance metrics (e.g., growth promotion for representative yeasts/molds).
• Typically supported by in-process QC (moisture control, solubility, pH after reconstitution, and growth-promotion testing), although exact tests and acceptance ranges are supplier-specific. Consult the CoA for this SKU.

User guidance:

• Always confirm the grams-per-liter preparation and target pH on the product label/CoA to match your application (enumeration vs enrichment vs propagation).

Reaction & Applications

This product is not a reagent for organic synthesis; it is a microbiological growth medium. Synthetic reaction applications do not apply.

Relevant research applications (literature/general):

• Cultivation, maintenance, and enumeration of yeasts and filamentous fungi from environmental, food/beverage, and fermentation samples.
• Growth promotion testing, starter culture propagation, and biomass production for downstream biochemical assays.
• Spoilage assessments and stress studies (e.g., osmotic or low-pH tolerance) in industrial microbiology contexts.

Practical considerations:

• Aeration/agitation improves yeast growth in shaken flasks; baffles or higher rpm may be beneficial (literature/general).
• Inoculum standardization (e.g., McFarland/comparable OD measurements) enhances reproducibility across experiments.
• For selective studies, MEB can be combined with antibiotics/antifungals or acidified after sterilization (where appropriate), but compatibility and sterilization filters should be validated experimentally (literature/general).

Reaction Conditions

Not applicable to chemical reaction conditions.

General microbiological preparation/usage conditions (literature/general; follow this product’s label/CoA for exact details):

• Dissolution: Add the manufacturer-recommended grams-per-liter of dehydrated medium to purified water with stirring; gentle warming (~40–50 C) can aid dissolution.
• Sterilization: Autoclave at 121 C for ~15 minutes (standard gravity cycle) unless otherwise specified. Avoid overheating to limit sugar caramelization.
• pH: Many MEB formulations exhibit mildly acidic pH after preparation; adjust only if method requires, and record any deviations.
• Incubation: Typical yeast cultivation at 25–30 C with shaking for aeration; molds often at 22–28 C in static or gently agitated conditions. Exact conditions depend on organism and objective.
• Supplements: Antibiotics, antifungals, or selective agents may be filter-sterilized and added post-autoclave when required; confirm compatibility.

Safety & Handling

Item-specific hazard data (from Product Data):

• GHS classification, signal word, hazard statements, pictograms: Not specified for this item; refer to SDS.

General safety considerations for dehydrated culture media (literature/general):

• Potential hazards: nuisance dust, mild irritation to eyes/respiratory tract; individuals sensitive to cereal/malt components may exhibit allergic responses.
• Primary PPE: lab coat, safety glasses/goggles, and appropriate gloves; use a dust mask/respirator if dust is generated. Handle powders in a ventilated area or fume hood to minimize inhalation.
• After reconstitution/sterilization: treat as biological culture medium. Once inoculated, handle as per institutional biosafety procedures. Decontaminate spent media by autoclaving or chemical disinfectants before disposal.
• Incompatibilities (general): strong oxidizers for dried organics; avoid moisture uptake during storage (keep container tightly closed).
• First aid (overview; defer to SDS):
  • Inhalation: move to fresh air; seek medical attention if symptoms persist.
  • Eye/skin contact: rinse with water; remove contaminated clothing.
  • Ingestion: rinse mouth; seek advice if unwell.
• Fire: Organic powder; use water spray, CO2, foam, or dry chemical as appropriate. Avoid dust dispersion (literature/general). Always consult the SDS for authoritative guidance.

Solvent Selection

This product is a dehydrated culture medium designed to be dissolved in water.

• Item-specific solvent recommendations: Not specified for this item; refer to CoA/Spec Sheet.

General/literature guidance:

• Primary solvent: Deionized/Distilled water. Use freshly prepared, low-conductivity water (ASTM Type II or better) to ensure pH and osmolality fall within expected ranges after dissolution and sterilization.
• Miscibility: Readily soluble in water; not intended for use with organic solvents.
• When to choose MEB vs alternatives:
  • MEB favors yeasts/molds due to malt-derived nutrients and mildly acidic conditions (literature/general).
  • Compare to Sabouraud Dextrose Broth (SDB) or Potato Dextrose Broth (PDB) when different carbohydrate profiles or pH targets are preferred (literature/general).
• Practical tips:
  • Warm water (~40–50 C) can aid dissolution; avoid excessive heating before sterilization to limit sugar degradation (literature/general).
  • Adjust final volume after autoclaving and cooling if evaporation is significant; check pH as needed (literature/general).

Storage & Reconstitution

Item-specific storage and shipping (from Product Data):

• Storage conditions: Room temperature.
• Shipped in: Normal.

Item-specific reconstitution details (g/L, pH targets): Not specified for this item; refer to CoA/Spec Sheet.

General/literature guidance for dehydrated media (not item-specific):

• Storage: Keep container tightly closed in a cool, dry place. Protect from humidity to prevent caking and performance drift. Avoid prolonged exposure to light/heat.
• Shelf life: Use within the supplier’s stated expiry; re-test performance if opened for extended periods.
• Reconstitution: Dissolve the label-specified grams-per-liter in purified water with stirring. Adjust pH only if method requires. Distribute to vessels and sterilize (e.g., 121 C for ~15 min).
• Post-sterilization handling: Allow to cool; add sterile-filtered supplements if required. Store prepared broth at 2–8 C if not used immediately; minimize storage time to reduce nutrient degradation (literature/general).

Research Use Only: As stated by the manufacturer, this product is for research use only.

Structure & Identity

• Item type: Complex, dehydrated microbiological growth medium (Malt Extract Broth; MEB), not a single defined molecule.
• Item-specific identifiers (from Product Data):
  • CAS: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
  • SMILES / InChI / InChIKey: Not applicable for complex mixtures.
• Structural description (general):
  • MEB typically comprises a mixture of saccharides and amino-nitrogen sources derived from malted cereals, plus minor minerals and growth factors (literature/general). It does not possess a single, well-defined 2D/3D molecular structure.
• Functional features (general):
  • Carbon/energy sources from malt extract (maltose, glucose, dextrins; literature/general).
  • Nitrogen/vitamin content from malt extract and, in some formulations, supplemental peptones (literature/general).

Synthetic Utility

Not applicable. Malt Extract Broth is a complex biological medium rather than a discrete chemical reagent. It is not used as a precursor, catalyst, or reagent in classical organic/inorganic synthesis.

If your work requires carbon sources for microbial biotransformations, MEB may serve as a cultivation matrix to enable whole-cell catalysis (e.g., yeast-mediated reductions) in a biological setting (literature/general), but it does not itself participate as a defined synthetic reagent.

Target Specificity

Not applicable. This product is not an antibody, enzyme inhibitor, or receptor ligand. It is a general-purpose growth medium that broadly supports many yeasts and filamentous fungi (literature/general) but does not exhibit target-binding specificity.