Methazolamide - Moligand™, 10mM in DMSO , Inhibitor of carbonic anhydrase 1;Inhibitor of carbonic anhydrase 12;Inhibitor of carbonic anhydrase 14;Inhibitor of carbonic anhydrase 4;Inhibitor of carbonic anhydrase 7, CAS No.554-57-4, Inhibitor of carbonic anhydrase 1;Inhibitor of carbonic anhydrase 12;Inhibitor of carbonic anhydrase 14;Inhibitor of carbonic anhydrase 4;Inhibitor of carbonic anhydrase 7

CAS: 554-57-4 Cat. No.: M408990 Formula: C₅H₈N₄O₃S₂ Molecular Weight: 236.3 EC Number: 209-066-7

GRADE & PURITY Moligand™, 10mM in DMSO

Synonyms

CL 8490 | Acetamide, N-[5-(aminosulfonyl)-3-methyl-1,3,4-thiadiazol-2(3H)-ylidene]-

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

Overview

Information

Methazolamide (CL 8490) is acarbonic anhydraseinhibitor withKiof 50 nM, 14 nM and 36 nM for hCA I, hCA II and bCA IV isoforms, respectively.
In vitro

Methazolamide is a carbonic anhydrase inhibitor with K_i of 50 nM, 14 nM and 36 nM for hCA I, hCA II and bCA IV isoforms, respectively. Methazolamide is of strength equal to acetazolamide, another carbonic anhydrase inhibitor used to treat irregular breathing disorders. However, methazolamide differs from acetazolamide in that it fails to activate Ca²⁺-dependent potassium channels in skeletal muscles.

In vivo

Methazolamide does not impair respiratory work performance in anesthetized rabbits.
Cell Data

cell lines：HCT116 and HT29 cells

Concentrations：

Incubation Time：

Powder Purity:≥99%

Specifications

Synonyms

CL 8490 | Acetamide, N-[5-(aminosulfonyl)-3-methyl-1,3,4-thiadiazol-2(3H)-ylidene]-

Specifications & Purity

Moligand™, 10mM in DMSO

Biochemical and Physiological Mechanisms

Methazolamide (CL 8490) is a carbonic anhydrase inhibitor with K_i of 50 nM, 14 nM and 36 nM for hCA I, hCA II and bCA IV isoforms, respectively.

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

Moligand™

Action Type

INHIBITOR

Mechanism of action

Inhibitor of carbonic anhydrase 1;Inhibitor of carbonic anhydrase 12;Inhibitor of carbonic anhydrase 14;Inhibitor of carbonic anhydrase 4;Inhibitor of carbonic anhydrase 7

Names and Identifiers

• Isomeric SMILES: CC(=O)N=C1N(N=C(S1)S(=O)(=O)N)C
• Molecular Weight: 236.3
• Reaxy-Rn: 232387
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=232387&ln=

Associated Targets(Human)

CA4 Tclin Carbonic anhydrase 4 (1 Activities)

Discover Target: CA4

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

CA12 Tclin Carbonic anhydrase 12 (1 Activities)

Discover Target: CA12

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

CA14 Tclin Carbonic anhydrase 14 (1 Activities)

Discover Target: CA14

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

CA7 Tclin Carbonic anhydrase 7 (1 Activities)

Discover Target: CA7

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Chemical and Physical Properties

• Solubility: Solubility (25°C) In vitro DMSO: 6 mg/mL (17.36 mM); Water: Insoluble; Ethanol: Insoluble
• Melt Point(°C): 208°C

References

1. Junrong Li, Yingjun Wang, Ziqi Fan, Panyang Tang, Mengting Wu, Hong Xiao, Zhenxing Zeng.  (2023)  Toxicity of Tetracycline and Metronidazole in Chlorella pyrenoidosa.  International Journal of Environmental Research and Public Health,  20  (4): (3623).  [PMID:36834317] [10.3390/ijerph20043623]

2. Da Wang, Zhe Yang, Yinning He, Shiwen Dong, Feilong Dong, Zhiqiao He, Xiaohui Lu, Lizhang Wang, Shuang Song, Jun Ma.  (2022)  Metribuzin and metamitron degradation using catalytic ozonation over tourmaline: Kinetics, degradation pathway, and toxicity.  SEPARATION AND PURIFICATION TECHNOLOGY,      [PMID:] [10.1016/j.seppur.2022.123028]

3. Haojiang Wang, Wenli Hu, Qiong Zheng, Wei Bian, Zian Lin.  (2017)  One-pot preparation of mercaptotetrazole-silica hybrid monoliths by the thiol-ene click reaction for mixed-mode capillary liquid chromatography.  JOURNAL OF SEPARATION SCIENCE,  40  (11): (2344-2354).  [PMID:28374911] [10.1002/jssc.201700220]