Methyl Green-Pyronin Staining Solution - BioReagent, ready-to-use, Suitable for Immunohistochemistry

Cat. No.: M743378

GRADE & PURITY BioReagent, Ready-to-use, Suitable for Immunohistochemistry(IHC)

Synonyms

Methyl Green-Pyronin Staining Solution

Storage

Protected from light,Room temperature

Shipped In

Normal

Application

IHC

Overview

Methylgreen-pyronine is a common dye used in tissue or cell staining to color the nucleus green or blue-green and the nucleoli in the cytoplasm and nucleus red. Methyl green can bind to DNA in the nucleus, thus producing nuclear staining; Pyronin can bind to RNA in the cytoplasm or nucleolus, so that the cytoplasm and nucleolus can be stained.
Operation process:
1 Sample handling
1.1 For paraffin sections: dewaxing in xylene for 2×5min; Anhydrous ethanol 5 min, 90% ethanol 2 min, 70% ethanol 2 min; Rinse with PBS for 2 min.
1.2 For frozen sections: Rinse with PBS for 2 min.
1.3 For cultured cells: fixed with 4% paraformaldehyde for more than 10 min and washed with PBS for 2×2 min.
2 methyl green-Pyronine staining for the above treated samples:
2.1 Methyl green-Pyronine dyeing solution staining for about 5 ~10min (the time can be adjusted according to the dyeing results and requirements). 2.2 Wash distilled water 2-3 times (each time for seconds).
3 Dehydration, transparency, sealing or other dyeing
3.1 Dehydration of acetone 3 times, 1~2sec(3 dips) each time.
3.2 Wash with acetone/xylene (1:1) twice, 1~2min each time. Xylene transparent 2×1~2min. 3.3 Seal the tablet with neutral gum or other sealant.
3.4 Microscopically, the nucleus appears green or blue-green, while the cytoplasm and nucleolus appear red.
Staining results: the nucleus is dyed green or blue-green, and the nucleoli in the cytoplasm and nucleus are dyed red.
Note:
1 Need to bring 4% polyformaldehyde, gradient ethanol, xylene, neutral gum or other tablets. 2 When using this kit for the first time, it is recommended to take 1 to 2 samples for pre-test.
Warm reminder:
For your own safety, please take good protection before using the reagent, such as wearing a lab coat, gloves, etc.

Specifications

Synonyms

Methyl Green-Pyronin Staining Solution

Specifications & Purity

BioReagent, ready-to-use, Suitable for Immunohistochemistry

Stability And Storage

Store at Room temperature long term (12 months). Upon receipt, it is recommended to aliquot. Store in the dark.

Storage

Protected from light, Room temperature

Shipped In

Normal

Grade

BioReagent, Ready-to-use, Suitable for Immunohistochemistry(IHC)

Chemical and Physical Properties

• Sensitivity: Light sensitive