Mouse IgG (AF660) - Isotype Control Antibody

Cat. No.: Ab225744
AVAILABLE TO ORDER
GRADE & PURITY Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. 0.5 mg/mL
Application
Flow Cytometry
Host species
Mouse
Isotype
Mouse IgG
Conjugation
AF660
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
25μg
Ab225744-25μg
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$99.90
100μg
Ab225744-100μg
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$199.90
500μg
Ab225744-500μg
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$599.90
Enter a quantity for the sizes you want to add.
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Why this grade

ExactAb™, Validated, 0.5 mg/mL ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

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Storage & shipping

Store at 2-8°C,Protected from light,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Mouse IgG; Polyclonal, Isotype Control, could be used for Flow Cytometry.

Specifications

Product Name
Mouse IgG (AF660) - Isotype Control Antibody
Synonyms
Mouse IgG
Specifications & Purity
ExactAb™, Validated, 0.5 mg/mL
Host species
Mouse
Conjugation
AF660
Excitation (nm)
Red Laser(633nm)
Emission(nm)
691nm
Ex/Em(nm)
Ex:663nm;Em:691nm
Grade
ExactAb™, Validated
Purification Method
Affinity chromatography
Concentration
0.5 mg/mL
Storage and Shipping
Storage
Store at 2-8°C,Protected from light,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Images

Mouse IgG (AF660) (Ab225744) - Flow Cytometry
Flow cytometry analysis of CD14 expression by human peripheral blood cells (right panel) compared to an isotype control (left panel). Human peripheral blood cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD14 Antibody (AF660) (Ab223473) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (AF660) (Ab225744).

Mouse IgG (AF660) (Ab225744) - Flow Cytometry
Flow cytometry analysis of CD14 expression by human peripheral blood monocytes (red) compared to an isotype control (blue). Human peripheral blood cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD14 Antibody (AF660) (Ab223473) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (AF660) (Ab225744). Black - Unlabelled control, cells without incubation with primary antibody.

Application
ApplicationDilution info
Flow Cytometry

Use at an assay dependent concentration.
Each lot of this antibody is quality control tested by flow cytometric analysis as negative control. Use at concentrations comparable to those of the speci

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateItem
ZJ25F0926740Certificate of AnalysisJun 14, 2026 Ab225744
ZJ25F0926741Certificate of AnalysisJun 14, 2026 Ab225744
ZJ25F0926742Certificate of AnalysisJun 14, 2026 Ab225744
Solution Calculators
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Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Validated grade guide → View ExactAb™ grade guide →

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