Narcissus pseudonarcissus lectin (NPA/NPL)

Cat. No.: N419214

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Overview

Features 

Ultrapure quality

Sugar specificity: D-GalNAc

Miogenic activity

Non-reactive with human erythrocytes

Lyophilized powder

Product description

Narcissus pseudonarcissus lectin (NPL) is isolated from daffodils. It exists as a dimeric protein with the molecular weight of 26 kDa composed by two subunits of 13 kDa each. The lectin has specificity toward alpha linked mannose. NPL is used in characterisation of early stages of apoptosis and is mitogenic for human lymphocytes. The lectin agglutinates rabbit erythrocytes but is non-reactive with human erythrocytes .

 NPL lectin is supplied as a white lyophilized powder from a buffer containing 10 mM CH3COONH4. The purity is determined by SDS-PAGE, which generates one band at 13 kDa, corresponding to the two isomers (the lectin dissociates below pH 5.0 and above pH 9.0)

Applications

Mitogenic studies of human lymphocytes

Agglutination studies

Directions for use

The lectin may be reconstituted with 2 ml of deionized water before use. Spin the vial gently until full dissolution. Aggregation is thought to occur in the presence of high concentrations of 2-mercaptoethanol. The solution may be reconstituted in this buffer to desired working concentration.

Shipping and storage

The product is shipped at -20°C however for over-the-day transport it may be shipped at ambient temperature. The lyophilized powder is stable for more than five years from production date when stored below -20°C. After reconstitution with deionized water, the solution may be stored frozen in working aliquots for up to 12 months.

Specifications

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.