Neomycin solution - with 10 mg/mL neomycin in 0.9% NaCl, 0.1 μm filter membrane filtration, BioReagent, suitable for cell culture

Cat. No.: N485930

GRADE & PURITY with 10 mg/mL neomycin in 0.9% NaCl, 0.1 μm filter membrane filtration, BioReagent, suitable for cell culture

Storage

Store at 2-8°C

Shipped In

Wet ice

Overview

Chemical structure: aminoglycoside

Application

Neomycin solution has been used:

as a supplement of autoclaved acidified water for bone marrow transplantation

for the selection of stable clones

as a component of artificial sea water (ASWO) medium to remove bacteria from O. tauri cultures

Biochem/physiol Actions

Neomycin is an aminoglycoside antibiotic, produced by Streptomyces containing a minimum of 85% neomycin B. It acts as a selection agent for prokaryotic cells transformed using the neo selectable marker gene. Neomycin trisulfate is used to study the cytototoxic side effects of antibiotics, platelet-derived growth factor responses in certain fibroblasts and extraction of nuclear phosphatidylinositol 4,5-bisphosphate-interacting proteins. This product is recommended for use in cell culture applications at 5 mL/L.

Mode of action: This product acts by binding to the 30S and 50S subunits, causing miscoding and inhibiting initiation and elongation during protein synthesis. Neomycin also blocks voltage-sensitive Ca2+ channels without affecting the Na+/Ca2+ antiporter in neurons.

Antimicrobial spectrum: Neomycin acts against both gram-positive and gram-negative bacteria

Disclaimer

This solution is formulated to contain 10 mg/mL neomycin in 0.9% sodium chloride. It is stable at 37 °C for 5 days and should be otherwise stored at 2-8°C.

Specifications

Specifications & Purity

with 10 mg/mL neomycin in 0.9% NaCl, 0.1 μm filter membrane filtration, BioReagent, suitable for cell culture

Biochemical and Physiological Mechanisms

Neomycin is an aminoglycoside antibiotic, produced byStreptomycescontaining a minimum of 85% neomycin B. It acts as a selection agent for prokaryotic cells transformed using the neo selectable marker gene. Neomycin trisulfate is used to study the cyto

Storage

Store at 2-8°C

Shipped In

Wet ice

This product requires cold chain shipping. Ground and other economy services are not available.

Chemical and Physical Properties

• Sensitivity: Prepare the solution as a 0.9% sodium chloride solution containing 10 mg/mL of neomycin. It can be stably stored for 5 days at 37 ° C, otherwise it should be stored at 2-8 ° C.