NGS Multiplex Oligos For Illumina (Index Primers Set II)

Cat. No.: N665671

Overview

Product content

Component	N665671-24T	N665671-96T
Adaptor for Illumina	60 μl	240 μl
Universal Primer for Illumina	30 μl	120 μl
Index 13-27 Primers for Illumina	12×10 μl	12×20 μl

Product Introduction

This kit provides the adaptor and primer used in second-generation sequencing library construction, designed for illumina platform library construction, index primer design can meet the needs of multiple high-throughput sequencing to prepare multiple library samples, the prepared library can be used for Illumina GAIIx, HiSacnSQ, HiSeq 2500/ 2000/1000 and MiSeq sequencing sequencing platform sequencing. 2000/1000 and MiSeq sequencing.

Provide your own instruments, reagents and consumables

1. Magnetic frame: DynaMagTM-2 is recommended.

2. DNA purification recovery kit.

3. DNA building kit.

4. Anhydrous ethanol.

5. Reaction tubes: It is recommended to use low adsorption PCR tubes with 1.5 ml centrifuge tubes;

6. Tip: It is recommended to use a high quality filter tip to prevent contamination of kits and library samples.

Pre-experiment Preparation and Important Notes

1. Adaptor is a double chain structure, do not leave it above room temperature to avoid unraveling and affecting the use.

2. Please centrifuge the Index primer briefly before uncapping so that the liquid collects at the bottom of the tube to avoid cross-contamination between different primers.
Usage process

Operation steps

Please follow the protocol of the second-generation sequencing rapid DNA library building kit for the use of the second-generation sequencing multi sample connector primer kit.

Adaptor for Illumina：

5´-/5Phos/ GATCGGAAGAGCACACGTCTGAACTCCAGT*C -3´

5´-ACACTCTTTCCCTACACGACGCTCTTCCGATC*T-3´

Universal Primer for Illumina：

5-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATC*T -3´

Index 13–27 Primers for Illumina：

	Index Primers for Illumina	Index
Index 13 Primer for Illumina	5-CAAGCAGAAGACGGCATACGAGATTGTTGACTGTGAC TGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´	AGTCAACA
Index 14 Primer for Illumina	5-CAAGCAGAAGACGGCATACGAGATACGGAACTGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´	AGTTCCGT
Index 15 Primer for Illumina	5-CAAGCAGAAGACGGCATACGAGATTCTGACATGTGAC TGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´	ATGTCAGA
Index 16 Primer for Illumina	5-CAAGCAGAAGACGGCATACGAGATCGGGACGGGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´	CCGTCCCG
Index 18 Primer for Illumina	5-CAAGCAGAAGACGGCATACGAGATGTGCGGACGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´	GTCCGCAC
Index 19 Primer for Illumina	5-CAAGCAGAAGACGGCATACGAGATCGTTTCACGTGAC TGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´	GTGAAACG
Index 20 Primer for Illumina	5-CAAGCAGAAGACGGCATACGAGATAAGGCCACGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´	GTGGCCTT
Index 21 Primer for Illumina	5-CAAGCAGAAGACGGCATACGAGATTCCGAAACGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´	GTTTCGGA
Index 22 Primer for Illumina	5-CAAGCAGAAGACGGCATACGAGATTACGTACGGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´	CGTACGTA
Index 23 Primer for Illumina	5-CAAGCAGAAGACGGCATACGAGATATCCACTCGTGAC TGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´	GAGTGGAT
Index 25 Primer for Illumina	5-CAAGCAGAAGACGGCATACGAGATATATCAGTGTGAC TGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´	ACTGATAT
Index 27 Primer for Illumina	5-CAAGCAGAAGACGGCATACGAGATAAAGGAATGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´	ATTCCTTT

Storage and Shipping

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads