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pHrodo™ Green Maleimide , CAS No.P638966
AVAILABLE TO ORDER
Storage
Protected from light,Store at -20°C,Desiccated
Shipped In
Ice chest + Ice pads
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Why this grade
for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Storage & shipping
Protected from light,Store at -20°C,Desiccated Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Product introduction：
The thiol-reactive pH-sensitive pHrodo™ Green Maleimide dye is suitable for the creation of bioconjugates to study endocytosis and phagocytosis. pHrodo™ Green dramatically increases fluorescence as the pH of its surroundings become more acidic.
• Use pH-sensitive pHrodo™ Green Maleimide to make pH-sensitive bioconjugates of your choice
• Get faster, more accurate results than with any other endocytosis or phagocytosis assay—no need for wash steps or quenchers
• Multiplex with red fluorescent markers such as RFP, pHrodo™ Red, and many others
The increase in fluorescence of pHrodo™ Green as pH changes from basic to acidic correlates with the acidification of intracellular vesicles, making it an ideal tool to study endocytosis or phagocytosis and their regulation by environmental factors, drugs, or pathogens. The spectral properties of pHrodo™ Green makes it useful for multi-color experiments. pHrodo™ Green can be detected using most standard green fluorescent filter sets and has been validated for use on a variety of platforms including flow cytometry, fluorescent microscopy, and high content screening (HCS). The lack of fluorescence of pHrodo™ Green in a typical extracellular environment eliminates the need for wash steps or quencher dyes in the experimental workflow.
pHrodo™ Green Maleimide is a thiol-reactive dye that can be used to create pHrodo™ Green bioconjugates in aqueous buffer. The maleimide reacts with free sulphydryl groups produced by the reduction of cysteines in proteins or peptides. Maleimides are particularly useful for labeling antibodies as the dye will not attach to the antibody binding site. This reaction will result in a stable conjugate that can be used in live cell assays or stored for later use.
pHrodo™ Green is also available in an amine-reactive form.In addition, pHrodo™ Red reactive dyes and ready-to-use conjugates are available as a color alternative with the same properties.
For Research Use Only. Not for human or animal therapeutic or diagnostic use.
• Use pH-sensitive pHrodo™ Green Maleimide to make pH-sensitive bioconjugates of your choice
• Get faster, more accurate results than with any other endocytosis or phagocytosis assay—no need for wash steps or quenchers
• Multiplex with red fluorescent markers such as RFP, pHrodo™ Red, and many others
The increase in fluorescence of pHrodo™ Green as pH changes from basic to acidic correlates with the acidification of intracellular vesicles, making it an ideal tool to study endocytosis or phagocytosis and their regulation by environmental factors, drugs, or pathogens. The spectral properties of pHrodo™ Green makes it useful for multi-color experiments. pHrodo™ Green can be detected using most standard green fluorescent filter sets and has been validated for use on a variety of platforms including flow cytometry, fluorescent microscopy, and high content screening (HCS). The lack of fluorescence of pHrodo™ Green in a typical extracellular environment eliminates the need for wash steps or quencher dyes in the experimental workflow.
pHrodo™ Green Maleimide is a thiol-reactive dye that can be used to create pHrodo™ Green bioconjugates in aqueous buffer. The maleimide reacts with free sulphydryl groups produced by the reduction of cysteines in proteins or peptides. Maleimides are particularly useful for labeling antibodies as the dye will not attach to the antibody binding site. This reaction will result in a stable conjugate that can be used in live cell assays or stored for later use.
pHrodo™ Green is also available in an amine-reactive form.In addition, pHrodo™ Red reactive dyes and ready-to-use conjugates are available as a color alternative with the same properties.
For Research Use Only. Not for human or animal therapeutic or diagnostic use.
Color:Green
Specifications
Storage
Protected from light, Store at -20°C, Desiccated
Shipped In
Ice chest + Ice pads
This product requires cold chain shipping. Ground and other economy services are not available.
Product Properties
| Extinction coefficient | 75000 |
|---|---|
| Reactive group | Thiol |
| Reactivity | Thiol |
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →✅ Certificate of Analysis (COA)
Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →🔬 Specification Sheet
Full quality attributes and acceptance criteria for this grade.
View spec sheet →Advanced Data
Certificates（CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Chemical and Physical Properties
| Solubility | DMSO(Dimethylsulfoxide) |
|---|
Solution Calculators
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