Product Introduction:
Blood samples are an important source of biological information in clinical research. They contain circulating proteins from multiple tissues and organs, which are involved in a wide range of biological processes and can be used as biomarkers or drug targets. However, blood samples have problems such as complex types of proteins, a large dynamic range, and a high proportion of high-abundance proteins, which bring great difficulties to proteome detection. This kit enriches circulating proteins in plasma samples based on the immunomagnetic bead capture method. In downstream proteomics detection, it can reduce the coverage of low-abundance protein signals by high-abundance proteins, thereby increasing the signal intensity of low-abundance proteins and improving the number of identified proteins and the repeatability of quantification.
Product Components and Storage Conditions:
项目号
| Component
| 12T
| 24T
| 48T
| Storage
|
P1456438A
| Magnetic beads
| 12T
| 24T
| 48T
| 4℃
|
P1456438B
| Incubation Buffer I
| 6.25 mL
| 12.5 mL
| 25 mL
| RT
|
P1456438C
| Incubation Buffer II
| 7.5 mL
| 15 mL
| 30 mL
| RT
|
P1456438D
| Washing Buffer
| 7.5 mL
| 15 mL
| 30 mL
| RT |
Product Features:
Higher: The detection depth of plasma proteins is increased to over 6000.
More stable: Combined with automated pretreatment instruments, it reduces manual operation errors and can process 96 samples at one time.
More valuable: With extensive cooperation, it achieves dual optimization of performance improvement and cost-effectiveness.
Operating Procedure:
1.Centrifuge the plasma sample (3000g, 10min), and take the supernatant for later use (if storage is needed, store it at -80°C for long-term preservation to avoid repeated freezing and thawing).
2.Take 50-100μL of centrifuged plasma, add 400μL of Incubation buffer I, then add 30μL of Magnetic beads, vortex to mix, and incubate at room temperature on a shaking mixer for 1h.
3.After incubation, use a magnetic rack to magnetically attract for 3min, and discard the supernatant.
4.Remove the EP tube, add 500μL of Incubation buffer II, gently invert up and down to mix several times, magnetically attract for 3min, and discard the supernatant.
5.Remove the EP tube, add 500μL of Washing Buffer, gently invert up and down to mix several times, magnetically attract for 3min, and discard the supernatant.
6.Add 50μL of Protein Lysis Buffer (P1408622) to the beads obtained in step 5.
7.It can be processed according to the conventional proteomics pretreatment steps in the laboratory, or our Proteomics Pretreatment Kit (P1456469) (to be purchased separately) can be used for processing.
Precautions:
1.Magnetic beads will precipitate after standing. Please shake gently and thoroughly before each use to keep the magnetic beads in a uniform suspension state.
2.During the storage and use of magnetic beads, operations such as freezing, drying and high-speed centrifugation should be avoided, as they may damage the structure of magnetic beads and affect their protein-binding ability.
3.This product is limited to scientific research use by professionals, and must not be used for clinical diagnosis or treatment, nor for food or drugs.
ZJ25F0927200