GRADE & PURITYEnzymoPure™?EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance.≥3 AU-A/g
EnzymoPure™, ≥3 AU-A/g EnzymoPure™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C,Protected from light Ships Wet ice Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 28 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Alcalase is an endo-protease of the serine type. It has a broad substrate specificity and can hydrolyze most peptide bonds within a protein molecule. It is active between pH 6.5 and 8.5 and has an optimum temperature of 60 °C. The enzyme is is used in detergent formulations to remove protein-based stains.
Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate .
Specifications
Product Name
Protease(Subtilisin), CAS No.9014-01-1
Synonyms
Alcalase® 3.0 T
Grade
EnzymoPure™
Specifications & Purity
EnzymoPure™, ≥3 AU-A/g
Biochemical and Physiological Mechanisms
Alcalase is a trademark of Novozymes Corp.
CAS
9014-01-1
Enzyme Commission Number
E.C. 3.4.21.62
Molecule Type
Enzyme
Storage and Shipping
Concentration
≥3 AU-A/g
Storage
Store at 2-8°C,Protected from light
Shipped In
Wet ice
Unit definition
K=kilogram, AU=Ansen unit, NPU=Novozymes protease unit, 1 AU=1NPU, ASNU=Aspartic acid unit, USP=USP Crystalline Trypsin Reference Standard enzyme quantity. The activity reference protease A standard yielded results consistent with the standard unit. 1ASNU
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.
Reconstitution Calculator
Reviews
Customer Reviews
Frequently Asked Questions
What is EnzymoPure??
EnzymoPure? is one of Aladdin Scientific's premium product lines, formulated for high-purity applications across multiple workflows. Compared to standard grades in the same workflow, EnzymoPure? products typically offer tighter specifications, more rigorous QC, and stronger lot-to-lot consistency.
How should this product be stored?
Store at 2–8 °C, protected from light. The material is light-sensitive — keep it in its original opaque or amber container.
How is this product shipped?
This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.
What are the CAS number, molecular formula and molecular weight?
The CAS Number is 9014-01-1.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
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