Protein A/G Elution Buffer (10×) - sterile-filtered, BioReagent

Cat. No.: E1456175

GRADE & PURITY Sterile-filtered, BioReagent

Synonyms

Protein A/G Elution Buffer

Storage

Store at 2-8°C

Shipped In

Wet ice

Application

Antibody Purification

Overview

  Aladdin IgG Elution Buffer (10×), pH 2.7, is a highly efficient, amine-based acidic elution buffer designed for the dissociation and recovery of antibodies from Protein A, Protein G, Protein A/G, and Protein L affinity supports. Supplied as a 10X concentrate, this ready-to-dilute formulation ensures convenient, high-yield antibody elution under low-pH conditions while maintaining compatibility with a wide range of immunoglobulin purification systems.

Key Features

• High-Efficiency Elution: Effectively dissociates bound antibodies from all major IgG-binding proteins under low-pH conditions.

• Ready-to-Dilute 10× Concentrate: Economical and space-saving; simply dilute with purified water to obtain 1× working solution.

• Broad Compatibility: Suitable for eluting antibodies in both singleplex and multiplex affinity purification workflows.

• Consistent Performance: Pre-adjusted to pH 2.7 to ensure reproducible elution profiles across applications.

• Versatile Application: Also effective in antigen-antibody or general protein-protein interaction studies.

Applications

• Antibody elution in IgG purification using Protein A, G, A/G, or L resins

• Ideal for research and development-scale immunoaffinity purification

• Compatible with manual and automated chromatography systems

• Suitable for elution in most antigen-antibody affinity purification setups

Instructions for Use

1. Dilute the 10× concentrate 1:9 with purified water to obtain 1× working solution.

2. Apply the 1× elution buffer to the affinity column or resin after binding and washing steps.

3. Collect eluted fractions and neutralize immediately with a suitable neutralization buffer (e.g., 1 M Tris-HCl, pH 9.0) to preserve antibody activity.

4. For optimal results, use in combination with Aladdin’s Protein A/G IgG Binding Buffer.

Notes

• Avoid prolonged exposure of antibodies to low-pH conditions to prevent denaturation.

• Neutralize eluted fractions promptly for maximum antibody recovery and stability.

• For sensitive antibodies, consider Aladdin’s Gentle Ag/Ab Elution Buffer (near-neutral, high-salt).

Specifications

Synonyms

Protein A/G Elution Buffer

Specifications & Purity

sterile-filtered, BioReagent

Stability And Storage

Store at 2-8 ℃ long term (12 months).

Storage

Store at 2-8°C

Shipped In

Wet ice

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

BioReagent, Sterile-filtered

Names and Identifiers

• PH: pH 2.7