GRADE & PURITYRUO?Research Use Only — for laboratory research, not diagnostic or therapeutic use. Use in R&D; do not use in clinical diagnosis or patient treatment.Recombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.Suitable for Flow Cytometry?Flow-cytometry grade — validated antibodies/reagents for FACS analysis. Use to stain and quantify cell populations by flow cytometry.0.5 mg/mL
Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous t
Host species
Rat
Specificity
Mouse CD146
Immunogen
Endothelial cell line TME-3H3
Positive Control
Flow Cytometry: bEnd.3
Conjugation
AF405
Excitation (nm)
Violet Laser(405nm)
Emission(nm)
422nm
Ex/Em(nm)
Ex:401nm;Em:422nm
Grade
Azide Free, ExactAb™, Recombinant, RUO, Suitable for Flow Cytometry
Action Type
ANTIBODY
Light Chain Type
kappa
Purification Method
Protein A purified
Concentration
0.5 mg/mL
Storage and Shipping
Storage
Store at 2-8°C,Protected from light,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Flow cytometry analysis of CD146 expression by bEnd.3 cells (red) compared to an isotype control (blue). Cells were stained with Recombinant CD146 Antibody (AF405) (Ab303141) at 0.1μg/test for 1 hour at 4°C. Blue – Rat IgG (AF405) (Ab190070). Black - Unlabelled control, cells without incubation with primary antibody.
Application
Application
Dilution info
Flow Cytometry
≤1.0 μg/test
For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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