GRADE & PURITYValidated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.5 μL/test
Recombinant CD16 Antibody, clone P93C38, could be used for Flow Cytometry.
Specifications
Product Name
Recombinant CD16 Antibody (APC) - Primary antibody, specific to Human CD16
Synonyms
CD16 | Gr1 | CD16A | Gr-1 | Fc fragment of IgG receptor IIIa | Ly-6C | Fc gamma RIII | Ly6G | FCG3 | Ly-6G | FCGR3 | ly-6G.1 | FcgRIII | Ly-6G/Ly-6C (Gr-1) | FCR-10 | lymphocyte antigen 6 complex, locus G | FCRIII | lymphocyte antigen 6G | FCRIIIA | IGFR3
Specifications & Purity
ExactAb™, Validated, 5 μL/test
Biochemical and Physiological Mechanisms
Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molec
Host species
Mouse
Specificity
Human CD16
Immunogen
Human polymorphonuclear leukocytes.
Positive Control
Flow Cytometry: Human peripheral blood lymphocytes.
Conjugation
APC
Excitation (nm)
Red Laser(633nm)
Emission(nm)
660nm
Ex/Em(nm)
Ex:650nm;Em:660nm
Grade
ExactAb™, Validated
Light Chain Type
kappa
Purification Method
Protein A purified
Concentration
5 μL/test
Storage and Shipping
Storage
Store at 2-8°C,Protected from light,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Images
Recombinant CD16 Antibody (APC) (Ab216122) - Flow Cytometry Flow cytometry analysis of CD16 expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant CD16 Antibody (APC) (Ab216122) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (APC) (Ab180309)
Recombinant CD16 Antibody (APC) (Ab216122) - Flow Cytometry Flow cytometry analysis of CD16 expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant CD16 Antibody (APC) (Ab216122) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (APC) (Ab180309). Black - Unlabelled control, cells without incubation with primary antibody.
Application
Application
Dilution info
Flow Cytometry
5 μL/test. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.
Reconstitution Calculator
Reviews
Customer Reviews
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