GRADE & PURITYValidated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.5 mg/mL
ExactAb™, Validated, 0.5 mg/mL ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C,Protected from light,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Recombinant F4/80 Antibody, clone P20A44, could be used for Flow Cytometry.
Specifications
Product Name
Recombinant F4/80 Antibody (Biotin) - Primary antibody, specific to F4/80
Synonyms
ADGRE1 antibody | Adhesion G protein coupled receptor E1 antibody | Adhesion G protein-coupled receptor E1 antibody | AGRE1_HUMAN antibody | Cell surface glycoprotein EMR1 antibody | Cell surface glycoprotein F4/80 antibody | DD7A5 7 antibody | Egf like m
Specifications & Purity
ExactAb™, Validated, 0.5 mg/mL
Biochemical and Physiological Mechanisms
Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
Host species
Rat
Specificity
F4/80
Immunogen
Murine macrophages
Positive Control
Flow Cytometry: C57BL/6 mouse bone marrow cells
Conjugation
Biotin
Grade
ExactAb™, Validated
Light Chain Type
kappa
Purification Method
Protein A purified
Concentration
0.5 mg/mL
Storage and Shipping
Storage
Store at 2-8°C,Protected from light,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Images
Recombinant F4/80 Antibody (Biotin) (Ab213900) - Flow Cytometry Two-color flow cytometric analysis of F4/80 expression on C57BL/6 mouse bone marrow cells. C57BL/6 mouse bone marrow cells were stained with CD11b Rat mAb (APC) (Ab178881) and either Rat IgG (Biotin) (Ab180310; Left panel) or Recombinant F4/80 Antibody (Biotin) (Ab213900; Right panel) at 0.2μg/test. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark.
Recombinant F4/80 Antibody (Biotin) (Ab213900) - Flow Cytometry Flow cytometry analysis of F4/80 expression by C57BL/6 mouse bone marrow cells (red) compared to an isotype control (blue). Cells were stained with Recombinant F4/80 Antibody (Biotin) (Ab213900) at 0.1 μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue – Rat IgG (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.
Application
Application
Dilution info
Flow Cytometry
≤0.3μg/test
For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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