GRADE & PURITYAnimal Free?Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material.Carrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Bioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.His Tag?His tag grade — recombinant protein bearing a His tag for affinity purification/detection. Use to purify, immobilize, or detect the tagged protein.≥95%(SDS-PAGE)
Expression System
HEK293
Accession #
P16284
Protein Tag
C-His
Expression system
HEK293
Endotoxin Concentration
<0.1 EU/μg
Bioactivity
1. Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells in the presence of 10 μg/mL PHA. The ED₅₀ value was lower than 5 ng/mL. 2. Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8×104 cells/well are added to CD31 coated plates (5μg/mL, 100 μL/well) in the presence of 10 μg/mL PHA, approximately 60-70% will adhere after 30 minutes at 37℃.
Animal Free,Carrier Free,Bioactive,ActiBioPure™,Azide Free,High Performance,His-Tag,≥95%(SDS-PAGE) ActiBioPure™,Animal Free,Azide Free,Bioactive,Carrier Free,High Performance,His Tag for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Purity
>95% SDS-PAGE.
Function
Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).
Post-translational
Phosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.
Specifications
Product Name
Recombinant Human CD31/PECAM-1 Protein, ＞95%(SDS-PAGE)
1. Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells in the presence of 10 μg/mL PHA. The ED₅₀ value was lower than 5 ng/mL. 2. Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8×104 cells/well are added to CD31 coated plates (5μg/mL, 100 μL/well) in the presence of 10 μg/mL PHA, approximately 60-70% will adhere after 30 minutes at 37℃.
94.5 kDa, under reducing conditions; 85.9 kDa, under non-reducing conditions.
Molecule Type
Protein
Storage and Shipping
Shape
Lyophilized
Reconstitution
Reconstitute in water to a concentration of 0.1-1.0 mg/ml.
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20~-80℃ for more than 1 year.
Images
Recombinant Human CD31/PECAM-1 Protein (rp143954) - Protein Bioactivity Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells in the presence of 10 μg/mL PHA. The ED₅₀ value was lower than 5 ng/mL.
Recombinant Human CD31/PECAM-1 Protein (rp143954) - Protein Bioactivity Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8×104 cells/well are added to CD31 coated plates (5μg/mL, 100 μL/well) in the presence of 10 μg/mL PHA, approximately 60-70% will adhere after 30 minutes at 37℃.
Recombinant Human CD31/PECAM-1 Protein (rp143954) - SDS-PAGE 3μg/lane of Recombinant Human CD31/PECAM-1 was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 94.5 kDa under reducing conditions and 85.9 kDa under non-reducing conditions.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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