GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Bioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.His Tag?His tag grade — recombinant protein bearing a His tag for affinity purification/detection. Use to purify, immobilize, or detect the tagged protein.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.≥95%(SDS-PAGE)
Accession #
P14174
Protein Tag
N-8His
Expression system
E. coli
Endotoxin Concentration
<1.0 EU/μg
Bioactivity
Immobilized Recombinant Human MIF Protein (rp214395) at 1.0 μg/mL can bind Recombinant MIF Antibody (Ab115362) with the EC50 of 4.3 ng/mL.
ActiBioPure™, Bioactive, Carrier Free, His Tag, PBS Only
Specifications & Purity
Carrier Free, Bioactive, ActiBioPure™, His Tag, PBS Only, ≥95%(SDS-PAGE)
Biochemical and Physiological Mechanisms
MIF (or macrophage migration inhibitory factor) was the first lymphokine/cytokine to be recognized in the pregenomics era. Regardless, it is one of the least understood of all inflammatory mediators. Human MIF is a 12.5 kDa, 115 amino acid (aa) nonglycosy
Bioactivity
Immobilized Recombinant Human MIF Protein (rp214395) at 1.0 μg/mL can bind Recombinant MIF Antibody (Ab115362) with the EC50 of 4.3 ng/mL.
12.0 kDa, under reducing conditions; 12.0 kDa, under non-reducing conditions.
Molecule Type
Protein
Storage and Shipping
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
Images
Recombinant Human MIF Protein (rp214395) - ELISA Immobilized Recombinant Human MIF Protein (rp214395) at 1.0 μg/mL can bind Recombinant MIF Antibody (Ab115362) with the EC50 of 4.3 ng/mL.
Recombinant Human MIF Protein (rp214395) - SDS-PAGE 3 μg/lane of Recombinant Human MIF Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 12.0 kDa.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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