GRADE & PURITYAnimal Free?Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material.Carrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Bioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.His Tag?His tag grade — recombinant protein bearing a His tag for affinity purification/detection. Use to purify, immobilize, or detect the tagged protein.≥95%(SDS-PAGE)
Accession #
P01580
Protein Tag
C-His
Expression system
HEK293
Endotoxin Concentration
<0.1 EU/μg
Bioactivity
Measured in antiviral assay using L929 cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is typically 0.2-1 ng/mL.
ActiBioPure™, Animal Free, Bioactive, Carrier Free, High Performance, His Tag
Specifications & Purity
Animal Free, Carrier Free, Bioactive, ActiBioPure™, High Performance, His-Tag, ≥95%(SDS-PAGE)
Biochemical and Physiological Mechanisms
Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JA
Bioactivity
Measured in antiviral assay using L929 cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is typically 0.2-1 ng/mL.
Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20~-80℃ for more than 1 year. Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Mouse IFN-gamma Protein (rp154254) - Protein Bioactivity Measured by its ability to inhibit the proliferation of HT-29 human colon cancer cells. The ED₅₀ for this effect is 4.8 ng/mL.
Recombinant Mouse IFN-gamma Protein (rp154254) - SDS-PAGE 2 μg/lane of Recombinant Mouse IFN-gamma Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining. As a result of different glycosylation, it migrates as approximately 15 & 18 kDa band in SDS-PAGE under reducing conditions and 15 & 18 & 32 & 36 kDa under non-reducing condition.
Application
Application
Dilution info
WB
1/4000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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