GRADE & PURITYAnimal Free?Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material.Carrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Bioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.His Tag?His tag grade — recombinant protein bearing a His tag for affinity purification/detection. Use to purify, immobilize, or detect the tagged protein.≥95%(SDS-PAGE)expressed in CHO, See COA
Accession #
Q99N32
Protein Tag
C-10His
Expression system
CHO
Endotoxin Concentration
<1.0 EU/μg
Bioactivity
Recombinant Mouse Klotho beta Protein (rp184968) cooperates with FGF21 to promote NIH-3T3 cell proliferation.The ED50 for this effect is 0.53 μg/mL in the presence of Recombinant mouse FGF21.
Immobilized Recombinant Human FGF19 Protein (rp145934) at 2.0 μg/mL can bind Recombinant Mouse Klotho beta Protein (rp184968) with the EC50 of 153.2 ng/mL.
Immobilized Recombinant Mouse Klotho beta Protein (rp184968) at 2.0 μg/mL can bind Recombinant Human FGF19 Protein (rp145934) with the EC50 of 1.25 μg/mL.
Animal Free,Carrier Free,Bioactive,ActiBioPure™,High Performance,His-Tag,≥95%(SDS-PAGE),expressed in CHO, See COA ActiBioPure™,Animal Free,Bioactive,Carrier Free,High Performance,His Tag for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
ActiBioPure™, Animal Free, Bioactive, Carrier Free, High Performance, His Tag
Specifications & Purity
Animal Free, Carrier Free, Bioactive, ActiBioPure™, High Performance, His-Tag, ≥95%(SDS-PAGE), expressed in CHO, See COA
Biochemical and Physiological Mechanisms
Klotho beta, a divergent structural member of the glycosidase I superfamily, is expressed primarily in the liver and pancreas, with lower expression in adipose tissue. Like Klotho, Klotho beta facilitates binding between FGF-19 subfamily members and their
Bioactivity
Recombinant Mouse Klotho beta Protein (rp184968) cooperates with FGF21 to promote NIH-3T3 cell proliferation.The ED50 for this effect is 0.53 μg/mL in the presence of Recombinant mouse FGF21.
Immobilized Recombinant Human FGF19 Protein (rp145934) at 2.0 μg/mL can bind Recombinant Mouse Klotho beta Protein (rp184968) with the EC50 of 153.2 ng/mL.
Immobilized Recombinant Mouse Klotho beta Protein (rp184968) at 2.0 μg/mL can bind Recombinant Human FGF19 Protein (rp145934) with the EC50 of 1.25 μg/mL.
124.9-150.0 kDa, under reducing conditions; 124.9-150.0 kDa, under non-reducing conditions.
Molecule Type
Protein
Storage and Shipping
Concentration
expressed in CHO, See COA
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20℃ for 6 months. Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Mouse Klotho beta Protein (rp184968) - Protein Bioactivity
Recombinant Mouse Klotho beta Protein (rp184968) cooperates with FGF21 to promote NIH-3T3 cell proliferation. The ED50 or this effect is 0.53 μg/mL in the presence of Recombinant mouse FGF21.
Recombinant Mouse Klotho beta Protein (rp184968) - Binding
Activity
Immobilized Recombinant Human FGF19 Protein (rp145934) at 2.0 μg/mL can bind Recombinant Mouse Klotho beta Protein (rp184968) with the EC50 of 153.2 ng/mL.
Recombinant Mouse Klotho beta Protein (rp184968) - Binding Activity
Immobilized Recombinant Mouse Klotho beta Protein (rp184968) at 2.0 μg/mL can bind Recombinant Human FGF19 Protein (rp145934) with the EC50 of 1.25 μg/mL.
Recombinant Mouse Klotho beta Protein (rp184968) - SDS-PAGE
3 μg/lane of Recombinant Mouse Klotho beta Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 124.9-159.5 kDa.
Application
Application
Dilution info
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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