Recombinant Mouse Ret Protein

Cat. No.: rp168533

GRADE & PURITY Animal Free, Carrier Free, Bioactive, ActiBioPure™, His Tag, ≥95%(SDS-PAGE)

Expression System

HEK293

Accession #

P35546

Protein Tag

C-His

Expression system

HEK293

Endotoxin Concentration

<0.1 EU/μg

Bioactivity

Measured by its binding ability in a functional ELISA. Serial dilution of immobilized rmRet (100 µL/well) was tested for binding with the GDNF-GFR alpha -1 complex using 4 ng/mL rhGDNF and 1 µg/mL rrGFR alpha -1/Fc Chimera. The concentration of immobilized Ret that produces 50% of the optimal binding response is found to be approximately 2-5 µg/mL.

Overview

Function
Receptor tyrosine-protein kinase involved in numerous cellular mechanisms including cell proliferation, neuronal navigation, cell migration, and cell differentiation in response to glia cell line-derived growth family factors (GDNF, NRTN, ARTN, PSPN and GDF15). In contrast to most receptor tyrosine kinases, RET requires not only its cognate ligands but also coreceptors, for activation. GDNF ligands (GDNF, NRTN, ARTN, PSPN and GDF15) first bind their corresponding GDNFR coreceptors (GFRA1, GFRA2, GFRA3, GFRA4 and GFRAL, respectively), triggering RET autophosphorylation and activation, leading to activation of downstream signaling pathways, including the MAPK- and AKT-signaling pathways. Acts as a dependence receptor via the GDNF-GFRA1 signaling: in the presence of the ligand GDNF in somatotrophs within pituitary, promotes survival and down regulates growth hormone (GH) production, but triggers apoptosis in absence of GDNF. Required for the molecular mechanisms orchestration during intestine organogenesis via the ARTN-GFRA3 signaling: involved in the development of enteric nervous system and renal organogenesis during embryonic life, and promotes the formation of Peyer's patch-like structures, a major component of the gut-associated lymphoid tissue. Mediates, through interaction with GDF15-receptor GFRAL, GDF15-induced cell-signaling in the brainstem which triggers an aversive response, characterized by nausea, vomiting, and/or loss of appetite in response to various stresses. Modulates cell adhesion via its cleavage by caspase in sympathetic neurons and mediates cell migration in an integrin (e.g. ITGB1 and ITGB3)-dependent manner. Also active in the absence of ligand, triggering apoptosis through a mechanism that requires receptor intracellular caspase cleavage. Triggers the differentiation of rapidly adapting (RA) mechanoreceptors. Involved in the development of the neural crest. Regulates nociceptor survival and size. Phosphorylates PTK2/FAK1.

Specifications

Product Name

Recombinant Mouse Ret Protein

Synonyms

Proto-oncogene Tyrosine-protein Kinase Receptor Ret | CDHF12MTC1 | CDHR16 | EC 2.7.10 | Hirschsprung disease 1 | Hirschsprung disease) | MEN2A | MEN2B | multiple endocrine neoplasia and medullary thyroid carcinoma 1 | ret proto-oncogene | RET transforming

Grade

ActiBioPure™, Animal Free, Bioactive, Carrier Free, His Tag

Specifications & Purity

Animal Free, Carrier Free, Bioactive, ActiBioPure™, His-Tag, ≥95%(SDS-PAGE)

Bioactivity

Measured by its binding ability in a functional ELISA. Serial dilution of immobilized rmRet (100 µL/well) was tested for binding with the GDNF-GFR alpha -1 complex using 4 ng/mL rhGDNF and 1 µg/mL rrGFR alpha -1/Fc Chimera. The concentration of immobilized Ret that produces 50% of the optimal binding response is found to be approximately 2-5 µg/mL.

Endotoxin Concentration

<0.1 EU/μg

Expression System

HEK293

Amino Acids

29-637 aa (F174S)

Sequence

LYFSRDAYWERLYVDQPAGTPLLYVHALRDAPGEVPSFRLGQHLYGVYRTRLHENDWIRINETTGLLYLNQSLDHSSWEQLSIRNGGFPLLTIFLQVFLGSTAQREGECHWPGCTRVYFSFINDTFPNCSSFKAQDLCIPETAVSSRVRENRPPGTFYHFHMLPVQFLCPNISVKYSLLGGDSLPFRCDPDCLEVSTRWALDRELREKYVLEALCIVAGPGANKETVTLSFPVTVYDEDDSAPTFSGGVGTASAVVEFKRKEGTVVATLQVFDADVVPASGELVRRYTNTLLSGDSWAQQTFRVEHSPIETLVQVNNNSVRATMHNYKLILNRSLSISESRVLQLAVLVNDSDFQGPGAGGILVLHFNVSVLPVTLNLPRAYSFPVNKRARRYAQIGKVCVENCQEFSGVSIQYKLQPSSINCTALGVVTSPEDTSGTLFVNDTEALRRPECTKLQYTVVATDRQTRRQTQASLVVTVEGTSITEEVGCPKSCAVNKRRPECEECGGLGSPTGRCEWRQGDGKGITRNFSTCSPSTRTCPDGHCDAVESRDANICPQDCLRADIVGGHERGERQGIKAGYGICNCFPDEKKCFCEPEDSQGPLCDALCRHHHHHH

Protein Tag

C-His

Accession #

P35546

Predicted molecular weight

68.4kDa

SDS-PAGE

110-120 kDa under reducing conditions.

Molecule Type

Protein

Storage and Shipping

Reconstitution

Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20~-80℃ for more than 1 year. Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

SDS-PAGE: Recombinant Mouse Ret Protein (rp168533)

Recombinant Mouse Ret Protein (rp168533) - SDS-PAGE
2 μg/lane of Recombinant Mouse Ret Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 90 - 110 kDa under reducing conditions and 90 - 110 & 180 - 220 kDa under non-reducing conditions.