GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Recombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.≥95%(SDS-PAGE)See COA
Carrier Free, Recombinant, ExactAb™, Azide Free, Validated, High Performance, ≥95%(SDS-PAGE), See COA
Biochemical and Physiological Mechanisms
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing.
Host species
Rat
Specificity
SIGLEC5/CD170
Positive Control
Flow: C57BL/6 mouse peritoneal macrophage
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, ExactAb™, High Performance, Recombinant, Validated
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A/G purified
Concentration
See COA
Storage and Shipping
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Two-color flow cytometric analysis of SIGLEC5/CD170 expression on C57BL/6 mouse peritoneal macrophages. C57BL/6 mouse peritoneal macrophages were stained with Recombinant F4/80 Antibody (AF647) (Ab213899) and either Rat IgG Isotype Control Antibody (Biotin) (Ab180310; Left panel) or Recombinant SIGLEC5/CD170 Antibody (Ab222909; Right panel) conjugated with biotin at 0.2 μg/mL for 1 hour at 4°C and detected with SA-PE (rp156150). Two-color flow cytometric dot plots showing the correlated expression of F4/80 versus SIGLEC5/CD170 (or Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable mouse peritoneal macrophages.
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