Recombinant Streptavidin Protein (AF594)

Cat. No.: rp181945

GRADE & PURITY Validated, ExactAb™, Ex:590nm, Em:618nm, 1.0 mg/mL

Accession #

P22629

Expression system

E. coli

Bioactivity

Binding Biotin

Specifications

Product Name

Recombinant Streptavidin Protein (AF594)

Synonyms

SA V1 | SA V2 | strepavidin | Streptavidin V1 | Streptavidin V2 | Streptavidin-AF594 | AF594-Streptavidin | SA-AF594

Grade

Em:618nm, Ex:590nm, ExactAb™, Validated

Specifications & Purity

ExactAb™, Validated, Ex:590nm, Em:618nm, 1.0 mg/mL

Bioactivity

Binding Biotin

Conjugation

AF594

Excitation (nm)

Yellow-Green Laser(594nm)

Emission(nm)

618nm

Ex/Em(nm)

Accession #

P22629

Predicted molecular weight

19 kDa

Molecule Type

Protein

Storage and Shipping

Concentration

1.0 mg/mL

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The product should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

IF: Recombinant Streptavidin Protein (AF594) (rp181945)

Recombinant Streptavidin Protein (AF594) (rp181945) - IF
IF analysis of beta Tubulin (red) in Hela cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab155823) dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (Biotin) (Ab179002) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Then incubated with Recombinant Streptavidin Protein (AF594) (rp181945) at a dilution of 1/500 for 1 hour at room temperature in the dark (red). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Flow Cytometry: Recombinant Streptavidin Protein (AF594) (rp181945)

Recombinant Streptavidin Protein (AF594) (rp181945) - Flow Cytometry
Flow cytometry analysis of CD45 expression by Jurkat cells (red) compared to an isotype control (blue). Cells were stained with CD45 Mouse mAb (Biotin) (Ab179532) at 0.05μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (AF594) (rp181945) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• IF/ICC:

  1:500 ;

• Flow Cytometry:

  ≤1.0 µg/test; For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume. Since applications vary, you should determine the optimum working

References

1. Yuping Liu, Beibei Zhang, Xueyuan Wu, Fan Wang, Zhiyi Yang, Mengyi Li, Kaixuan Sheng, Yue Yan, Liang Zhu, Hui Jing, Yanmin Wu, Lili Hu, Yanyan Yu, Chenglin Li.  (2025)  A facile liquid biopsy assay for highly efficient CTCs capture and reagent-less monitoring of immune checkpoint PD-L1 expression on CTCs with non-small cell lung cancer patients.  BIOSENSORS & BIOELECTRONICS,      [PMID:39929085] [10.1016/j.bios.2025.117236]

2. Xiaohui Yang, Yixian Li, Josh Zixi Lee, Yuanmin Sun, Xin Tan, Yijie Liu, Yang Yu, Huiqiang Li, Xue Li.  (2024)  A Highly Sensitive Dual-Drive Microfluidic Device for Multiplexed Detection of Respiratory Virus Antigens.  Micromachines,  15  (6): (685).  [PMID:38930655] [10.3390/mi15060685]

3. Ziyang He, Yonghong Meng, Mei Liu.  (2024)  A multicolor aptasensor based on target-induced anti-etching gold nanorod for visible detection of aflatoxin B1.  MICROCHEMICAL JOURNAL,      [PMID:] [10.1016/j.microc.2024.110659]

4. Yunzhi Zhao, Ying Hao, Min Cui, Na Li, Bao Sun, Yu Wang, Haiyan Zhao, Cong Zhang.  (2024)  An electrochemical biosensor based on DNA tetrahedron nanoprobe for sensitive and selective detection of doxorubicin.  BIOELECTROCHEMISTRY,      [PMID:38271768] [10.1016/j.bioelechem.2024.108652]

5. Jiawei Chen, Gan Zhang, Xiaoyue Xiao, Daofeng Liu, Juan Peng, Yonghua Xiong, Weihua Lai.  (2024)  Bifunctional bovine serum albumin modification driven sensitivity-enhanced lateral flow immunoassay for small molecule hazards monitoring in food.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,      [PMID:39476895] [10.1016/j.ijbiomac.2024.136915]

6. Yangyang Li, Riyue Shu, Cheng Zeng, Jing Wang, Lin Zhang, Zhe Tang.  (2025)  Bio-inspired AS1411-Functionalized membrane for specific and non-destructive capture of tumor cells.  JOURNAL OF MEMBRANE SCIENCE,      [PMID:] [10.1016/j.memsci.2025.123833]

7. Danyu Wang, Xin Zhou, Mengyu Huang, Jie Duan, Yue Qiu, Hua Yi, Yang Wang, Huimin Xue, Jiali Zhang, Qiuxia Yang, Hua Gao, Zhenzhen Guo, Kaixiang Zhang.  (2024)  Cascade Enzymes Confined in DNA Nanoanchors for Antitumor Therapy.  ACS Applied Materials & Interfaces,      [PMID:39265065] [10.1021/acsami.4c09835]

8. Huinan Chen, Jingyao Song, Yuanyuan Li, Dongmei Deng, Yuchen Song, Xiaoli Zhu, Liqiang Luo.  (2024)  Cascade signal amplifying strategy for ultrasensitive detection of tumor biomarker by DNAzyme cleaving mediated HCR.  SENSORS AND ACTUATORS B-CHEMICAL,      [PMID:] [10.1016/j.snb.2024.136466]

9. Junqiu Liu, Zhaidong Liu, Chunqin Zhao, Yuting Jiao, Baohong Li, Jiaju Shi, Zichao Chen, Zhen Zhang.  (2024)  Coaxial dual-path electrochemical biosensing and logic strategy-based detection of lung cancer-derived exosomal PD-L1.  Nanoscale,      [PMID:38630023] [10.1039/D4NR00412D]

10. Boyu Yang, Chao Li, Yongshuo Ren, Weichen Wang, Xiangxiang Zhang, Xiaojun Han.  (2024)  Construction of the Glycolysis Metabolic Pathway Inside an Artificial Cell for the Synthesis of Amino Acid and Its Reversible Deformation.  Journal of the American Chemical Society,      [PMID:39042264] [10.1021/jacs.4c06227]

11. Yan Liu, Yujie Li, Chen Shao, Ping Wang, Xiaoxuan Wang, Runhe Li.  (2024)  Curcumin-based residue-free and reusable photodynamic inactivation system for liquid foods and its application in freshly squeezed orange juice.  FOOD CHEMISTRY,      [PMID:38968711] [10.1016/j.foodchem.2024.140316]

12. Qiudi Xu, Ke Liu, Yu He, Lan Wang, Zefan Lu, Zhongxuan Liu, Tao Zhang.  (2024)  Digital microfluidic chip with photopatterned reactive sites for direct biomolecules immobilization and magnetic beads-free immunoassay.  SENSORS AND ACTUATORS B-CHEMICAL,      [PMID:] [10.1016/j.snb.2024.136893]

13. Hang Ao, Wencheng Xiao, Wenrui Hu, Jie Wu, Huangxian Ju.  (2024)  DNA Conformation-Regulated Hemin Switch for Lab-on-Chip Chemiluminescent Detection of an Antibody Secreted from Hybridoma Cells.  ANALYTICAL CHEMISTRY,      [PMID:39503400] [10.1021/acs.analchem.4c04122]

14. Mingxia Lin, Huiyi Yang, Qinglan Li, Huanxin Xiao, Shilin Jiang, Jinhui Liang, Xiping Cui, Suqing Zhao.  (2024)  Dual lateral flow assay based on PdRu nanocages for human Papillomavirus detection.  JOURNAL OF COLLOID AND INTERFACE SCIENCE,      [PMID:38908288] [10.1016/j.jcis.2024.06.002]

15. Rongsheng Hu, Shaowei Lin, Fajun Li, Jiaqing Shen, Yinong Xie, Baichang Deng, Youyu Zhang, Zhaogang Dong, Jinfeng Zhu.  (2024)  Exploring Aptamer-Based Metasurfaces for Label-Free Plasmonic Biosensing of Breast Tumor-Derived Exosomes.  Advanced Optical Materials,  12  (28): (2401180).  [PMID:] [10.1002/adom.202401180]

16. Shihong Zhu, Daohang Du, Zhimin Zhao, Xianfeng Chu, Daoxiang Su, Shuli Yu, Ting Tao, Yong Jiang, Zhifei Wang.  (2024)  Fabrication of functional interface on magnetic beads via various amino acids and their application in chemiluminescent immunoassay as carrier.  COLLOIDS AND SURFACES B-BIOINTERFACES,      [PMID:39527882] [10.1016/j.colsurfb.2024.114364]

17. Danyi Zhao, Mai Hu, Chenghao Hu, Di Wang, Hailun Chen, Yangwei Ou, Rongli Liu, Xiaoyang Li, Long Wu, Peng Liu, Zhiwei Shen, Qi Chen.  (2024)  Multivalent bifunctional nanobody to enhance the sensitivity of direct competitive chemiluminescence immunoassay for the detection of microcystin LR in lake water.  TALANTA,      [PMID:39467444] [10.1016/j.talanta.2024.127080]

18. Yu-E Gao, Qing Huang, Yue Huang, Li-Xia Yan, Wen-Long Wang, WenFeng Zhao, Yong-Wei Feng, Xiao-Dong Huang, Yi Zhang.  (2024)  Nanoparticle-mediated light-driven LAMP combined with test strips for sensitive and rapid visual detection of antibiotic resistance genes.  JOURNAL OF HAZARDOUS MATERIALS,      [PMID:39752829] [10.1016/j.jhazmat.2024.136981]

19. Wenli Zhu, Miao Hu, Guanghua Su, Weifang Jiang, Hancong Du, Kaxi Chen, Jinfang Nie, Lang Zhang, Xuehui Tang, Yun Zhang.  (2025)  Nanoprobe-free nanosensor with Tyndall-effect readout for colorimetric detection of alkaline phosphatase.  MICROCHEMICAL JOURNAL,      [PMID:] [10.1016/j.microc.2025.113295]

20. Liping Wu, Min Qing, Zhaode Mu, Jing Zhou, Jianli Zuo, Lijuan Bai.  (2024)  New electrocatalyst of Ni6MnO8@AB/PtNPs with efficient synergistic enhancement in electrochemical aptasensor for sensitive detection of neuron specific enolase.  MICROCHEMICAL JOURNAL,      [PMID:] [10.1016/j.microc.2024.110639]

21. Huanxin Xiao, Weiguang Chen, Mingxia Lin, Shilin Jiang, Xiping Cui, Suqing Zhao.  (2024)  Rapid immunoassay for dual-mode detection of HPV16 and HPV18 DNA based on Au@PdPt nanoparticles.  Analytical Methods,      [PMID:38463013] [10.1039/D3AY02307A]

22. Jianjian Ji, Yingcai Xiong, Keyu Tao, Tao Li, Weiying Ou, Yinghui Zhou, Wenyang Zhang, Ruogu Qi, Shouchuan Wang.  (2024)  Resveratrol inhibits respiratory syncytial virus replication by targeting heparan sulfate proteoglycan.  Food & Function,      [PMID:38270052] [10.1039/D3FO05131E]

23. Ziru Feng, Mengmeng Yan, Tengfei Li, Wenjun Zhang, Chao Zhu.  (2025)  Screening specific aptamer pairs towards bovine lactoferrin for its sandwich application.  Food Bioscience,      [PMID:] [10.1016/j.fbio.2025.105911]

24. Jialuo Ai, Yixuan Huang, Zhaoyi Yin, Yingshan Deng, Ling Yan, Jingwen Liao, Guoyan Liang, Chong Chen, Yunbing Chang, Cairong Xiao, Jiale Zhou, Zurong Zhu, Chengli Liu, Zhuo Jiang, Chengyun Ning, Zhengao Wang.  (2024)  SEA Anemone-inspired Conducting Polymer Sensing Platform for Integrated Detection of Tumor Protein Marker and Circulating Tumor Cell.  Advanced Healthcare Materials,      [PMID:38767216] [10.1002/adhm.202401305]

25. Yi Zhang, Jing Cao, Jie Zhou, Wen-Long Wang, Zhenghua Xu, Yong-Wei Feng, Xiao-Dong Huang, Feng-De Yu, Xin Yang.  (2024)  Strand displacement amplification combining photothermal-colorimetric dual mode test strip for ultra-sensitive detection of patulin.  MICROCHEMICAL JOURNAL,      [PMID:] [10.1016/j.microc.2024.112109]

26. Qiang Zhang, Junlei Zhao, Anqi Han, Xiaonan Zhang, Mingya Yang, Hui Li, Benli Yu, Guosheng Zhang, Sheng Zhou.  (2024)  Ultrasensitive cancer cell sensing based on tapered optical fiber operating near the dispersion turning point.  SENSORS AND ACTUATORS B-CHEMICAL,      [PMID:] [10.1016/j.snb.2024.135473]