Sabouraud Dextrose Broth (USP) - BioReagent

Cat. No.: S1528343

GRADE & PURITY BioReagent

Storage

Room temperature

Shipped In

Normal

Overview

For cultivation of molds and yeasts. Preparation method: Weigh 30 g of this product in 1 L of purified water, heat to boiling until completely dissolved, dispense, autoclave at 121°C for 15 minutes, and set aside for use.

Specifications

Specifications & Purity

BioReagent

Storage

Room temperature

Shipped In

Normal

Grade

BioReagent

Product Line

Biological Products

Application Protocols

Item-specific validated protocols are not provided. The following is literature/general guidance for research labs; adjust to your SOP and consult the CoA/label for lot-specific details.

Basic broth preparation (literature-typical):

1. Weigh the dehydrated medium to give ~50 g/L total solids (broth; see note). Add to ~80–90% final volume of purified water with stirring.
2. Heat gently (≤60 °C) to aid dissolution. Do not boil. Bring to final volume.
3. Check and adjust pH if required by your method (commonly ~5.6 at 25 °C).
4. Dispense into suitable vessels. Sterilize by autoclaving at 121 °C for 15 minutes. Allow to cool to handling temperature.
5. Aseptically add supplements (e.g., antibiotics) if used. Mix thoroughly.

Yeast culture (example workflow):

• Inoculate sterile SDB with a single colony or a defined inoculum to an initial OD600 of 0.05–0.1.
• Incubate at 30 °C, 200 rpm in baffled flasks (fill volume ≤20% to improve aeration).
• Monitor growth by OD600 or dry cell weight; harvest in exponential phase as needed.

Fungal filamentous culture (example):

• Inoculate with spores or mycelial fragments; incubate static or with gentle shaking (100–150 rpm) at 25–30 °C. Use larger vessels or baffles to minimize pellet overgrowth if dispersed morphology is desired.

Note: The 50 g/L figure and pH are literature-typical for SDB; for this specific item, exact preparation instructions are Not specified for this item; refer to CoA/Spec Sheet.

Biological Roles

Sabouraud Dextrose Broth (SDB) is not a biological macromolecule or metabolite; rather, it is a culture environment engineered to favor fungal growth.

Literature/general context:

• Selectivity mechanism: high dextrose concentration and an acidic pH (~5.6, literature) inhibit many bacteria while maintaining compatibility with yeasts and filamentous fungi. Peptone provides amino acids, peptides, and growth factors.
• Organisms commonly supported: Candida spp., Saccharomyces spp., Aspergillus spp., and other environmental/foodborne fungi used in research laboratories.
• Use cases in microbiology: biomass amplification before downstream assays (e.g., microscopy, metabolite analysis, enzyme assays), preservation/maintenance cultures, and inoculum preparation for fermentation studies.
• Physiological notes: carbohydrate-rich, low-pH environments can influence morphology (e.g., yeast vs. hyphal forms for dimorphic fungi) and metabolite profiles; medium choice should match the biological question.

Limitations:

• Reduced suitability for bacteria due to acidity; if bacterial co-culture is needed, neutral pH media (e.g., TSB) or buffered systems are preferred.
• Nutrient richness can mask auxotrophies; use defined media when genotype-phenotype resolution is required.

Buffer Applications

Not typically applicable. Sabouraud Dextrose Broth is a nutrient medium rather than a defined buffering system, and it is not used for precise pH buffering in biochemical assays.

Literature/general notes:

• The medium’s acidity (commonly around pH 5.6 after preparation, literature) contributes to selectivity but does not provide strong buffering capacity.
• If a controlled buffer is required for enzymology or protein work with fungal extracts, prepare a separate buffer system (e.g., citrate, acetate, or phosphate) and use SDB only for the culture phase.

Green Alternatives

As a water-based biological medium, Sabouraud Dextrose Broth already avoids organic solvents and halogenated reagents commonly targeted for green substitution. Greener-chemistry considerations mainly focus on waste minimization and energy use during sterilization.

Sustainability considerations (general):

• Energy: autoclaving is energy-intensive. Where validated and appropriate, terminal filter-sterilization with 0.22 µm membranes can reduce steam usage for heat-stable but supplement-heavy preparations (balance against filter waste).
• Waste: prepare only the volume needed to reduce disposal of unused sterile media. Consolidate sterilization cycles to improve autoclave load efficiency.
• Packaging: transfer bulk powder to airtight, reusable containers with desiccant to prolong shelf life and reduce product loss from caking.

Alternative media choices (contextual, literature):

• For yeast biomass with lower sugar load, YNB (Yeast Nitrogen Base) with defined carbon sources can reduce nutrient overuse and provide more controlled effluent composition.
• For environmental isolates, lower-dextrose formulations or buffered media (e.g., Malt Extract Broth) may reduce acidification and downstream neutralization needs.

Trade-offs:

• More defined media can enhance reproducibility and reduce nutrient waste but may require additional components and cost; rich media like SDB maximize growth for many fungi but generate higher COD in effluent.

Pharmaceutical Uses

No therapeutic or clinical uses are claimed for this product. It is for research use only.

Literature/general context:

• USP context: the name “(USP)” reflects a formulation that aligns with monograph-style preparations used in pharmacopeial testing workflows (e.g., growth media for certain microbiological examinations). For this specific item, compliance or certification status is Not specified for this item; refer to CoA/Spec Sheet.
• Typical roles in pharmaceutical quality-control laboratories (general): environmental monitoring isolates maintenance, microbial limits testing (fungal enrichment), and method development/validation studies for antifungal assessments, where permitted by SOPs.

Formulation/excipient status:

• Not used as an excipient in drug products; role is limited to laboratory media for cultivating microorganisms in non-clinical settings.

Physical Properties

Item-specific (from Product Data):

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Storage Conditions: Room temperature
• Shipped In: Normal

Not specified for this item; refer to CoA/Spec Sheet (do not construe as specifications):

• Moisture content, pH after reconstitution, osmolality, and color are not specified here.

Literature/general properties for Sabouraud Dextrose Broth (information for planning only, not product specifications):

• Typical composition (literature): peptone ~10 g/L and dextrose ~40 g/L; no agar. Final pH commonly ~5.6 at 25 °C to suppress many bacteria and favor fungi.
• Solubility: readily soluble in purified/distilled water; forms a clear to slightly opalescent solution after full dissolution.
• Physical form (dehydrated): free-flowing powder, hygroscopic; tends to cake if exposed to humidity.
• Refractive index, BP/MP, density, logP: not applicable/reported for this complex mixture.

Practical notes (general):

• Dissolution is endothermic and aided by gentle heat and stirring; avoid overheating to prevent Maillard-type browning of carbohydrates with peptides.
• Filter sterilization or autoclaving are typical depending on downstream use (see Reaction Conditions/Application Protocols for guidance).

Quality & Grades

Item-specific (from Product Data):

• Grade/Purity: BioReagent

Interpretation and implications (general):

• BioReagent grade typically indicates suitability for biological research applications with controlled bioburden and impurity profiles appropriate for culture and molecular biology workflows. However, exact acceptance criteria (e.g., growth promotion performance, sterility, pH range) are item-specific and must be confirmed on the CoA/Specification Sheet.
• USP designation in the product name indicates the formulation aligns with United States Pharmacopeia guidance for Sabouraud Dextrose media (literature). Certification/compliance status for this lot is Not specified for this item; refer to CoA/Spec Sheet.

What to check on receipt (best practices):

• Verify lot-specific growth promotion test (GPT) results (e.g., C. albicans, A. brasiliensis, S. cerevisiae) if provided.
• Confirm reconstituted pH, clarity, and sterility method (autoclave/filtration) consistent with your validated method.
• Inspect packaging integrity and dryness; caking or discoloration can indicate moisture uptake or overheating during storage.

Stabilizers/antimicrobials:

• No antimicrobial inhibitors or stabilizers are indicated in the Product Data. If antibiotic supplements (e.g., chloramphenicol) are required, add aseptically after sterilization and cooling.

Reaction & Applications

This product is a microbiological culture medium; it is not used as a reagent in chemical reactions. The relevant “applications” are biological/culture workflows.

Research and laboratory applications (literature/general):

• Cultivation and maintenance of fungi and yeasts, including Candida spp., Saccharomyces spp., and filamentous fungi.
• Selective enrichment of fungi from clinical- or environmental-type samples (research-only context in this catalog) due to the low pH and high dextrose content.
• Sterility checks and bioburden assessments of materials and surfaces in research labs; often used alongside antibiotics to suppress bacterial overgrowth.
• Morphology and sporulation studies when paired with agar (Sabouraud Dextrose Agar); broth phase supports biomass build-up prior to plating.
• Antifungal susceptibility screening (broth microdilution or macrodilution) when aligned with your lab’s validated method; note that standardized methods may specify alternative media.

Practical tips (general):

• Use freshly prepared, clear medium; discard if turbid before inoculation.
• Pre-warm to incubation temperature to minimize lag phase.
• For environmental enrichment, consider adding antibacterial supplements (e.g., chloramphenicol) aseptically after sterilization and cooling.
• Document lot numbers and GPT results to maintain traceability across studies.

Reaction Conditions

Conventional chemical reaction parameters do not apply. The relevant “conditions” are media preparation and sterilization steps.

Literature/general preparation conditions (for planning; verify against your SOP and CoA/label):

• Reconstitution ratio (broth): commonly prepared at approximately 50 g/L total solids (derived from ~10 g/L peptone + ~40 g/L dextrose, literature). Dissolve in purified water to final volume.
• pH: typical target ~5.6 at 25 °C (literature). Adjust if your method specifies.
• Sterilization: autoclave at 121 °C for 15 minutes. Alternatively, dissolve, adjust pH, filter-sterilize (0.22 µm) if heat-labile supplements are to be added; note that peptone-rich media filter more slowly.
• Incubation: 25–30 °C for molds; 30–37 °C for many yeasts (literature ranges). Agitation 150–250 rpm in baffled flasks improves aeration for yeast cultures.
• Expected outcomes: clear to slightly opalescent sterile medium pre-inoculation; turbidity development and potential pelleting or mycelial mats post-inoculation depending on organism and agitation.

Quality checks:

• Perform negative control (uninoculated) incubations to verify sterility of each batch.
• Optionally verify growth promotion with a known strain per lab SOP.

Safety & Handling

Item-specific hazard information (from Product Data):

• Signal Word: Not specified for this item; refer to SDS.
• H-Statements: Not specified for this item; refer to SDS.
• GHS Classification: Not specified for this item; refer to SDS.
• Pictograms: Not specified for this item; refer to SDS.

General safety (literature/practice; consult the SDS as the authoritative source):

• Expected hazards: dehydrated culture media powders may cause mechanical eye irritation and respiratory tract irritation if inhaled; ingestion is not intended. Not intrinsically pathogenic, but the medium will support microbial growth.
• Critical biosafety consideration: once rehydrated and inoculated, the culture will contain live microorganisms. Handle plates/broths and waste according to institutional biosafety guidelines for the organism involved (e.g., BSL-1/2). Decontaminate by autoclaving or validated disinfectants before disposal.
• Incompatibilities: avoid strong oxidizers and reactive carbonyl chemistry with amines/sugars at elevated temperature (Maillard reactions). Keep dehydrated medium dry; hygroscopic absorption degrades flow and may alter performance.
• PPE: lab coat, safety glasses, and appropriate gloves. Use dust control (weighing boat with cover, fume hood or downdraft station) to minimize aerosolization of powder.
• First-aid (overview):
  • Inhalation: move to fresh air; seek medical attention if symptoms persist.
  • Eye contact: rinse cautiously with water for several minutes; remove contact lenses if present and easy to do.
  • Skin contact: wash with soap and water.
  • Ingestion: rinse mouth with water; do not induce vomiting; seek advice if unwell.
• Fire: carbohydrate content can burn; use water spray, CO2, or dry chemical. Combustion may produce CO, CO2, and nitrogen oxides. Firefighters should wear SCBA.

Solvent Selection

This product is a dehydrated microbiological medium intended to be dissolved in water; conventional solvent screening (as for organic synthesis) is not applicable.

Practical guidance (general):

• Primary solvent: purified/distilled water (ASTM Type 1/2 or equivalent). Avoid high ionic strength or chlorine/chloramine residuals that can inhibit growth.
• Dissolution: add powder gradually to ~80–90% of the final water volume with vigorous stirring; gently warm (≤60 °C) if needed. Bring to final volume after complete dissolution.
• pH: literature-typical finished pH is acidic; adjust only if your method requires it. For most fungal applications, do not neutralize unless specified by your SOP.
• Sterilization: autoclave at 121 °C for 15 minutes (literature) unless your validated method specifies filtration (0.22 µm) for heat-labile additives. Cool to ~45–50 °C before aseptically adding supplements.

Comparison to alternatives (general considerations):

• Versus Potato Dextrose Broth (PDB): SDB provides a more defined peptone nitrogen source; PDB is plant-derived and can promote sporulation in some fungi.
• Versus Yeast Extract-Peptone-Dextrose (YPD): YPD contains yeast extract, often supports faster yeast growth but is richer; SDB’s acidic pH is more selective against bacteria.

Storage & Reconstitution

Item-specific (from Product Data):

• Storage Conditions (dehydrated powder): Room temperature
• Shipped In: Normal

Best practices (general; verify with CoA/label):

• Keep the dehydrated medium tightly sealed in a cool, dry place. Protect from humidity; include a desiccant in secondary containment after opening. Avoid strong light and heat.
• After reconstitution and sterilization: store unused sterile broth at 2–8 °C. Use promptly (typically within 1–2 weeks) to minimize caramelization, pH drift, and contamination risk. Warm to room/Incubation temperature before use.
• Do not freeze reconstituted medium unless validated, as freeze–thaw may precipitate components and alter performance.

Reconstitution guidance (literature-typical; not product specifications):

• Target concentration: approximately 50 g/L total solids for broth. Dissolve in purified water with stirring; adjust pH if required by your SOP (commonly ~5.6 at 25 °C). Sterilize by autoclaving 121 °C for 15 minutes or filter-sterilize where appropriate.

Stability indicators:

• Discard if the dehydrated powder cakes severely, shows discoloration, or if prepared medium is turbid before inoculation, forms unexpected precipitates, or shows off-odors.

Research Use Note:

• For research use only. Not for diagnostic procedures, therapeutic use, or human/animal consumption.

Structure & Identity

Brief description: Sabouraud Dextrose Broth (USP) is a complex, undefined microbiological growth medium formulated for the cultivation of fungi and yeasts. It is a mixture (not a single molecule), so conventional single-compound identifiers do not apply.

• Item-specific (from Product Data):
  • SKU: S1528343
  • Product Name: Sabouraud Dextrose Broth (USP)
  • Grade: BioReagent
  • Research Use: For research use only
• Structure identifiers for a pure compound (SMILES, InChIKey, CAS, CID) are not applicable to a complex medium.
  • CAS: Not specified for this item; refer to CoA/Spec Sheet.
  • SMILES: Not applicable (mixture)
  • InChIKey: Not applicable (mixture)
• Molecular formula / molecular weight: Not applicable to a complex medium; overall values are not defined.

Literature/context (general):

• Typical constituents (broth, no agar) include an enzymatic digest of protein (peptone) and high dextrose (glucose) content; the formulation yields an acidic medium that favors fungi over most bacteria.
• 2D “structure” description in words: a homogeneous aqueous solution of peptides, amino acids, and carbohydrates (primarily glucose) after reconstitution in water; no discrete molecular structure.

Synthetic Utility

Not applicable to chemical synthesis. Sabouraud Dextrose Broth is a biological medium and does not serve as a reagent or building block in organic or inorganic synthesis.

If your workflow blends chemistry with microbiology (general guidance):

• Use SDB to generate fungal biomass, then proceed with chemical extraction or biotransformation steps using appropriate solvents and buffers outside the culture medium.
• For biocatalysis with fungal enzymes or whole cells, confirm whether the acidic, sugar-rich environment aligns with the target enzyme’s stability; often, a switch to a defined buffer post-culture is advisable.

Target Specificity

Not applicable. This product is not an antibody, probe, or targeted reagent; it is a general-purpose fungal/yeast growth medium. No antigen/epitope, species reactivity, clone, or isotype information applies.