Schaedler Broth - BioReagent

Cat. No.: S1528578

GRADE & PURITY BioReagent

Storage

Room temperature

Shipped In

Normal

Overview

For enrichment of anaerobic bacteria

Specifications

Specifications & Purity

BioReagent

Storage

Room temperature

Shipped In

Normal

Grade

BioReagent

Product Line

Biological Products

Application Protocols

General preparation and use (literature guidance; follow label/CoA for this item’s exact instructions):

• Reconstitution: Weigh the dehydrated medium according to the label-specified grams per liter. Disperse in high-purity water with gentle heating and stirring until fully dissolved.
• pH: Adjust only if directed by the label. Typical Schaedler-type broths target near-neutral pH after sterilization (literature), but follow product specifications.
• Sterilization: Dispense into suitable vessels (tubes, bottles), cap loosely, and autoclave using a validated liquid cycle. Allow to cool.
• Supplements: If required, aseptically add sterile-filtered hemin, vitamin K1, blood/lysed blood, and additional reducing agents after autoclaving.
• Pre-reduction: Boil briefly and cool under anaerobic gas, or place in an anaerobic chamber/jar to equilibrate until redox indicator (if present) is fully reduced.
• Inoculation and incubation: Inoculate with test organisms, minimize air exposure, and incubate under strict anaerobic conditions at the strain-appropriate temperature.
• Quality checks: Perform growth-promotion testing with reference anaerobes and include negative controls. Examine turbidity, gas production (if relevant), and odor cautiously; confirm purity by streaking onto anaerobic agar.

Item-specific preparation mass, supplements, and parameters: Not specified for this item; refer to CoA/Spec Sheet.

Biological Roles

Schaedler Broth itself has no intrinsic biological role as a biomolecule; instead, it functions as a nutrient environment that supports microbial physiology.

Literature/general context:

• Nutrient supply: Provides amino acids, peptides, carbohydrates (e.g., dextrose), minerals, and growth factors (e.g., hemin, vitamin K1 in supplemented versions) to support biosynthesis, energy production, and redox balance in anaerobes.
• Redox management: Inclusion of reducing components (e.g., cysteine/cystine systems) and low-oxygen handling lowers the redox potential (Eh), enabling growth of oxygen-sensitive organisms.
• Metabolic pathways supported: Fermentative and anaerobic respiratory pathways prevalent in Bacteroides, Clostridium, and related genera, including carbohydrate fermentation to organic acids and gases (strain-dependent; literature).
• Community recovery: In mixed cultures, rich broth facilitates resuscitation of stressed cells from clinical or environmental samples prior to selective plating.
• Indicator use: Optional redox dyes (e.g., resazurin) provide a visual proxy for oxygen exposure, guiding handling to maintain anaerobiosis.

Note: Specific organism performance depends on exact formulation and handling (pH, temperature, atmosphere). Users should perform growth-promotion testing with relevant strains under their operating conditions.

Buffer Applications

This product is not designed as a defined buffering system. While prepared broths possess some intrinsic buffering capacity from phosphates, peptides, and bicarbonate systems (formulation-dependent), Schaedler Broth is intended for microbial cultivation rather than analytical buffering.

• If a defined buffer is needed (e.g., for enzyme assays or pH-controlled experiments), use dedicated buffers (phosphate, HEPES, MOPS) and validate compatibility with the organisms of interest.
• For anaerobic culture, maintain pH via proper gas mix (e.g., CO2-containing atmospheres for bicarbonate systems) and by avoiding overgrowth/acidification; consider periodic subculture or pH monitoring.

Item-specific buffer composition and pH range: Not specified for this item; refer to CoA/Spec Sheet.

Green Alternatives

While Schaedler Broth is an aqueous microbiological medium rather than an organic solvent, sustainability considerations still apply.

Greener practice options (general, literature):

• Water-only solvent: Preparation uses water as the sole solvent, inherently minimizing volatile organic compound (VOC) usage.
• Animal-origin minimization: Select formulations emphasizing plant-derived peptones (e.g., soy) where performance is equivalent, to reduce reliance on animal by-products. Performance equivalence must be verified by growth-promotion testing.
• Energy-efficient sterilization: Optimize autoclave loads and cycles, and use validated liquid cycle parameters to avoid overprocessing. Consider filtration sterilization for heat-labile supplements (hemin/vitamin K1) to reduce repeated autoclave cycles.
• Waste reduction: Prepare only the volume needed; aliquot to minimize expiry-related discards. Use reclosable, moisture-tight containers to extend powder shelf life and reduce waste.
• Gas consumption: When using anaerobic gas packs or cylinders, plan batch preparation to minimize purge cycles. Use anaerobic chambers with catalyst maintenance for efficient O2 removal.

Balanced tradeoffs:

• Plant peptones can vary lot-to-lot in growth performance versus animal peptones; extensive QC may be needed.
• Lower-temperature sterilization reduces energy but may not achieve required F0; do not compromise sterility assurance. Validate any process changes.

Pharmaceutical Uses

No therapeutic or clinical diagnostic claims are made for this product. For research use only.

General, non-clinical applications in pharmaceutical and biomanufacturing settings (literature/industry practice):

• QC microbiology: Enrichment of anaerobic challenge organisms for growth-promotion testing of media and for method suitability studies (e.g., sterility test simulations), when validated by the user.
• Environmental monitoring isolates: Resuscitation and maintenance of anaerobic or fastidious environmental isolates encountered in facility monitoring programs.
• Process development: Cultivation of anaerobic production strains or consortia in early research phases to explore metabolite profiles (non-GMP), prior to transfer into defined fermentation media.
• Raw material bioburden assessments: Recovery medium for stressed anaerobes in supplier qualification studies.

Regulatory note: If used in regulated workflows, growth-promotion and suitability must be demonstrated per compendial or internal SOPs. Composition and performance criteria for this specific item are Not specified for this item; refer to CoA/Spec Sheet.

Physical Properties

As a complex dehydrated culture medium, Schaedler Broth does not have single-compound physical constants.

Item-specific values (this product):

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not applicable (mixture).
• Solubility in water: Expected to be soluble/dispersible (general for media); item-specific value not specified.
• pH (prepared medium): Not specified for this item; refer to CoA/Spec Sheet.

Literature/general values (for typical Schaedler-type media; not product specifications):

• Physical form: Free-flowing, tan-to-beige dehydrated powder that dissolves in purified water to form a clear to slightly opalescent broth after sterilization.
• Typical working pH: ~7.0 ± 0.2 at 25 °C (literature).
• Osmolality: Formulation-dependent; often isotonic to mildly hypertonic relative to water (literature).
• Redox potential: Designed for low Eh under reduced/anaerobic conditions, especially when supplemented with reducing agents and pre-reduced (literature).

Notes:

• Exact values vary by manufacturer and lot. Validate pH, clarity, and sterility after preparation. If using for strict anaerobes, pre-reduction (oxygen removal) is critical to achieve appropriate redox potential.

Quality & Grades

• Grade: BioReagent (as listed for this item). This denotes suitability for biological research applications and routine microbiological culture work. BioReagent grade typically emphasizes low bioburden and consistent performance characteristics relevant to life science workflows.

What BioReagent grade implies (general):

• Formulation consistency appropriate for growth and recovery of targeted organisms.
• Manufacturing controls to minimize contaminants that could inhibit growth (e.g., heavy metals, inhibitory residues), though exact limits are not specified here.
• Suitable for research, method development, and QC microbiology (non-clinical). Not intended for diagnostic use unless validated by the end user.

Stabilizers/additives: Not specified for this item; refer to CoA/Spec Sheet.

Lot qualification (recommended user checks):

• Growth promotion testing with representative anaerobes (e.g., Bacteroides spp., Clostridium spp.) and facultatives as controls.
• pH verification after dissolution and sterilization.
• Clarity and color check post-autoclave; verify absence of precipitates beyond expected formulation behavior.

Documentation: For exact composition, preparation mass per liter, and QC release criteria, consult the item’s CoA/Spec Sheet.

Reaction & Applications

Chemical reaction content is not typically applicable to microbiological media. Instead, the relevant “applications” focus on microbial cultivation.

Microbiological applications (literature/general; not product-specific claims):

• Enrichment and maintenance of obligate anaerobes (e.g., Bacteroides, Prevotella, Fusobacterium, Clostridium) under anaerobic atmospheres.
• Recovery of fastidious organisms from mixed specimens prior to isolation on solid anaerobic media.
• Growth of facultative organisms in parallel as controls, with or without oxygen restriction.
• Use as a base broth for supplementation with hemin, vitamin K1, blood/lysed blood, and reducing agents to enhance recovery of highly fastidious anaerobes.
• Pre-reduced broth for susceptibility testing workflows (user-validated), where consistent redox potential and nutrient profile are important.

Practical tips (literature):

• Pre-reduce medium by boiling and cooling under an anaerobic gas, or equilibrate in an anaerobic chamber for several hours prior to inoculation.
• Include redox indicators (e.g., resazurin) as desired to visually monitor oxygen ingress (colorless when reduced; pink when oxidized).
• Inoculate and incubate without shaking for strict anaerobes; minimize headspace oxygen using filled tubes with tight closures or butyl stoppers.
• Validate growth-promotion with relevant strains and target incubation temperatures (often 35–37 °C for human-associated anaerobes).

Reaction Conditions

Classical chemical reaction conditions are not applicable. Below are literature-based microbiological cultivation conditions relevant to Schaedler-type broths (general guidance; not product specifications):

• Atmosphere: Strictly anaerobic for obligate anaerobes (e.g., 80–90% N2, 5–10% CO2, 5–10% H2, or commercial anaerobic systems). Include catalyst for O2 removal if using H2/CO2 systems.
• Temperature: Commonly 35–37 °C for human-associated anaerobes; environmental isolates may prefer different temperatures (validate per strain).
• Incubation: Static (no shaking) to limit oxygen ingress; fill tubes to minimize headspace when appropriate. Duration ranges from 24–72 h or longer for slow growers.
• Supplements: Add hemin and vitamin K1 aseptically after sterilization if not included in the base. Reducing agents (e.g., cysteine) can be adjusted to target redox potential.
• Monitoring: Use resazurin or similar indicators to track redox state; avoid repeated exposure to air. Subculture onto anaerobic agar for isolation and purity checks.

Note: Exact preparation mass per liter, target pH, and sterilization parameters for this item are Not specified for this item; refer to CoA/Spec Sheet and SDS. Always validate growth-promotion under your lab’s conditions.

Safety & Handling

Safety designations for complex media can vary. Always consult the SDS for authoritative information.

Item-specific hazard data:

• Signal word (GHS): Not specified for this item; refer to SDS.
• Hazard statements / Pictograms / Classification: Not specified for this item; refer to SDS.

General laboratory safety (applicable to dehydrated media and prepared broths):

• PPE: Wear lab coat, safety glasses, and appropriate gloves (e.g., nitrile). Avoid inhalation of dust from dehydrated media; minimize aerosol generation.
• Handling: Use in a clean environment to prevent contamination. For anaerobic work, handle under anoxic conditions (anaerobic chamber, jars, or gas-mix hoods) after sterilization and pre-reduction.
• Incompatibilities: Strong oxidizers may degrade organic constituents; moisture may cause clumping or microbial contamination. Keep container tightly closed.
• First aid (general): If inhaled, move to fresh air. If on skin/eyes, rinse with water. If ingested, rinse mouth. Seek medical attention if symptoms persist. Defer to SDS.
• Microbiological risk: Once inoculated, broths may contain pathogenic organisms. Treat all cultures and waste as biohazardous; follow BSL-appropriate practices. Autoclave or chemically disinfect before disposal.
• Special risks: Prepared anaerobic broths may off-gas under certain conditions; vent containers carefully in anaerobic jars/chambers. Avoid pressure build-up during autoclaving by loosely capping prior to sterilization.

Solvent Selection

Schaedler Broth is formulated for reconstitution in water.

• Primary solvent: High-purity water (Type 1 or Type 2 laboratory water). Avoid water with residual chlorine, oxidants, or high TOC that may inhibit growth.
• Miscibility: The dehydrated powder is designed to fully dissolve/disperse in water with heating if required; not intended for organic solvents.
• Deoxygenation (critical for anaerobes):
  • Boil the medium gently and cool under an oxygen-free gas blanket (N2, N2/CO2, or 80/20 N2/H2) to drive off dissolved O2 (literature, general practice).
  • Use pre-reduced anaerobically sterilized (PRAS) techniques; reduce and equilibrate in an anaerobic chamber when possible.
• When to choose Schaedler vs alternatives:
  • Choose Schaedler for broad recovery of fastidious and strict anaerobes, especially when using hemin/vitamin K1 supplements.
  • Alternatives: Brain Heart Infusion (BHI) or Tryptic Soy Broth (TSB) for general aerobes/facultatives; Reinforced Clostridial Medium (RCM) or Wilkins–Chalgren for certain anaerobic applications (literature comparison).

Small comparison (literature, general):

• Schaedler: Broad-spectrum anaerobe support; benefits from hemin/Vit K1; low redox design.
• RCM: Emphasizes clostridia; rich carbohydrate and reducing system.
• BHI/TSB: Aerobic/facultative growth; not inherently optimized for strict anaerobes.

Storage & Reconstitution

Item-specific storage:

• Storage conditions: Room temperature (as provided in Product Data). Protect from moisture and light. Keep container tightly sealed.
• Shipped in: Normal (ambient) conditions.

General storage guidance (dehydrated media):

• Maintain in a cool, dry place. Use desiccant in secondary containment if ambient humidity is high. Avoid temperature cycling and direct sunlight.
• Reseal promptly after opening to minimize moisture uptake and clumping. Record the opening date and manage by first-opened/first-used.

Reconstitution guidance (follow label/CoA for exact directions; no item-specific quantities provided here):

• Dissolve the label-specified mass per liter in high-purity water with gentle heating and stirring.
• If directed, adjust pH before sterilization. Distribute into suitable containers, cap loosely, and autoclave using a validated cycle. Allow to cool.
• Aseptically add any heat-labile supplements (e.g., hemin, vitamin K1) after sterilization. For anaerobic use, pre-reduce the broth before inoculation by equilibrating under an anaerobic atmosphere.

Stability (general):

• Dehydrated powder: Stable until the labeled expiry when stored properly.
• Prepared medium: Best used fresh. If storage is necessary, refrigerate as appropriate and protect from oxygen ingress; shelf life depends on supplements and handling. Item-specific stability: Not specified for this item; refer to CoA/Spec Sheet.

Research use only.

Structure & Identity

Schaedler Broth is a complex, undefined microbiological growth medium formulated to support fastidious and obligate anaerobes. It is not a single chemical entity and therefore lacks a single molecular formula or structure.

• SKU: S1528578
• Product name: Schaedler Broth
• Chemical identity: Complex mixture (peptones, extracts, carbohydrates, salts, reducing components; exact composition is manufacturer-dependent)
• Molecular formula: Not applicable to complex media
• Molecular weight: Not applicable to complex media
• SMILES / InChI / InChIKey: Not applicable to complex media
• Structural features (general, literature): Typically contains protein hydrolysates (e.g., casein/soy peptones), yeast extract, dextrose, sodium chloride, reducing agents (e.g., L-cystine/cysteine), and hemin and/or vitamin K1 for enhanced anaerobe growth. Some variants include a redox indicator (e.g., resazurin).
• 2D description in words: Heterogeneous blend of organic and inorganic nutrients; no discrete molecular structure.

Item-specific identifiers (CAS, CID): Not specified for this item; refer to CoA/Spec Sheet.

Synthetic Utility

Not applicable. Schaedler Broth is a microbiological growth medium and is not used as a reagent or building block in chemical synthesis.

For users seeking chemical function in anaerobic processes: employ appropriate catalysts, reductants, and solvents in the chemical domain. For microbial biotransformations, Schaedler Broth can serve as the cultivation matrix for the organism performing the transformation, but it does not itself participate as a reactant.

Target Specificity

Not applicable. This product is not an antibody, enzyme inhibitor, or small-molecule ligand. It is a general-purpose anaerobic growth medium designed to support a wide range of anaerobic and fastidious microorganisms. No antigen/epitope specificity is relevant.