The Small RNA Ladder (10-50nt, 9 bands) produced by Aladdin, also known as microRNA Marker, miRNA Marker, small RNA ladder, or small RNA Marker, is a type of Small RNA Molecular Weight Marker. It contains nine single-stranded small RNA bands with lengths of 10, 15, 20, 25, 30, 35, 40, 45, and 50nt respectively. Both the 5' and 3' ends of each small RNA are hydroxyl groups, making it suitable as a molecular weight reference for routine electrophoretic analysis and detection of single-stranded small RNAs.
The small RNA bands of this product are evenly and finely distributed, facilitating the comparison of small RNA sizes.
This product is an ideal choice for analyzing 10-50nt small RNAs in urea-denatured polyacrylamide gels (10%-20%) with urea concentrations of 7M, 7.5M, or 8M. The 30nt band in this product has higher brightness, making it easier to distinguish the bands.
This product is formulated in DEPC water (DNase and RNase free) containing 50% deionized formamide. Loading 2μl per well allows for clear observation of small RNA bands, and one package of this product can be used 50 times.
Precautions：
1. There is a large amount of RNase in the environment, and this product is prone to degradation due to RNase contamination during use. Strict precautions must be taken to avoid RNase contamination during operation. It is advisable to wear a mask when pipetting this product, minimize the time that the Small RNA Ladder is exposed to the air, cover the cap immediately after use, avoid repeated freeze-thaw cycles as much as possible, and it is recommended to appropriately aliquot this product when using it for the first time.
2. When this product is only used as a molecular weight marker for single-stranded RNA, it is not recommended for accurately determining the length of small RNAs. This is because the composition of different nucleotides in small RNAs can also have a certain impact on electrophoretic mobility.
3. This product is solely for scientific research use by professional personnel. It must not be used for clinical diagnosis or treatment, nor for food or pharmaceutical purposes, and must not be stored in ordinary residential premises.
4. For your safety and health, please wear a lab coat and disposable gloves during operation.
Instructions for Use：
1. Prepare a urea-denatured polyacrylamide gel with an appropriate concentration (7M, 7.5M, or 8M).
2. For denaturing gel electrophoresis: Mix 2 μl of Small RNA Ladder with 2 μl of 2× RNA Loading Buffer (R743508), incubate at 95°C for 1 minute, immediately place in an ice-water bath to cool, then load the entire 4 μl mixture into the well of the urea-denatured polyacrylamide gel to start electrophoresis.
3. After electrophoresis, take out the gel and place it in a clean container (e.g., a glass petri dish of appropriate size). Rinse with DEPC water for 1–2 minutes, then add approximately 30 ml of nucleic acid staining solution. Stain on a shaker at room temperature for 15 minutes at 25 rpm. Finally, wash with DEPC water 2–3 times, 2–5 minutes each time. The stained electrophoresis results can then be observed using gel imaging equipment.
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