BioReagent, 5% in deionized water BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Room temperature Ships Normal Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
During tissue sectioning, when tissues contain bone or calcified foci, calcium-containing tissues are not suitable for direct paraffin embedding and sectioning. This is due to the density difference between calcium and paraffin, making it difficult to obtain intact sections. For calcium-containing tissues, fixation is preferably performed first, followed by decalcification, or the two processes can be carried out simultaneously. During decalcification, acidic substances break the cross-links between protein molecules in the tissue and release hydrophilic groups that can bind to water molecules, resulting in tissue swelling. After tissue decalcification, the acid in the tissue must be neutralized immediately to rapidly deacidify and neutralize the tissue, ensuring section quality and staining efficacy. Sodium Sulfate Aqueous Solution (5%) is a commonly used post-treatment solution for decalcified bone tissue, with overnight treatment.
Protocol (for reference only):
1. During bone tissue decalcification, the specimen thickness should not be excessive, generally approximately 5 mm.
2. Wash the decalcified tissue with distilled water 3 times, 20 minutes each time.
3. Transfer the tissue to a 5% aqueous sodium sulfate solution with a volume 20–30 times that of the tissue, and treat for 24 h.
4. Rinse the tissue with distilled water several times.
Precautions:
1. Appropriate heating can accelerate the treatment process, and the temperature should generally not exceed 37–40 ℃.
2. Water washing alone is not recommended, as it easily causes tissue swelling.
3. For your safety and health, please wear a laboratory coat and disposable gloves during operation.
4. This product is for research use only; strictly prohibited for other purposes.
Appendix:
Ethanol treatment method: Treat with 70% ethanol, replace every 1 h, and repeat twice.
Comprehensive hazard, handling, storage, and regulatory compliance document.
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| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ26F0434339 | Certificate of Analysis | Apr 20, 2026 | S1520407 |
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