This product is a sterile normal saline solution of Heparin Sodium, a classic mucopolysaccharide polysulfate anticoagulant. Its anticoagulant mechanism involves specific binding to antithrombin III (AT III) in plasma, significantly enhancing AT III's inactivation of various coagulation factors (particularly factor Xa and factor IIa, i.e., thrombin), thereby achieving potent anticoagulation. Additionally, heparin sodium inhibits platelet aggregation. This product is intended for scientific research purposes only and must not be used for clinical diagnosis, treatment, or any human applications.
Scope and Limitations
1. Suitable Experiments
2. Unsuitable Experiments
Coagulation profile tests (e.g., PT, APTT determination): Heparin interferes with the intrinsic and common coagulation pathways, leading to severely distorted results.
General hematological examination and blood smears: Heparin can cause leukocyte aggregation, affecting differential counts; it also produces a light blue background on Wright-stained blood smears, interfering with microscopic observation.
Clinical diagnosis.
Usage Instructions
Please read all precautions before operation and strictly adhere to aseptic techniques.
1. General Anticoagulation Ratio: The recommended volume ratio of anticoagulant to whole blood is 1:49 to 1:50.
Example: Take 1 mL of this product, add 49–50 mL of freshly collected whole blood, and immediately invert gently 8–10 times to ensure thorough mixing and anticoagulation. Avoid vigorous shaking to prevent hemolysis.
2. Experiment-Specific Adjustment: Some experiments may require different anticoagulant ratios. Please strictly follow the specific experimental protocol or literature.
3. Operational Procedure
3.1 Wear a lab coat, mask, and disposable sterile gloves.
3.2 Using a sterile syringe or pipette, draw the calculated volume of this product and add it to a clean blood collection tube or container.
3.3 Collect fresh blood into the container containing this product.
3.4 Mix immediately, gently, and thoroughly.
Precautions
1. Use Restriction: This product is for research use only. It is strictly prohibited for human use or clinical diagnosis.
2. Experiment Selection: Before use, confirm that the intended experiment aligns with this product's suitable applications. Avoid use in coagulation tests and blood smear preparation in particular.
3. Aseptic Technique: Although this product is sterile, opening and handling must be performed in a clean environment using sterile instruments to prevent microbial contamination.
4. Precise Ratio: The anticoagulant-to-blood ratio is critical for accurate results. Use calibrated precision pipetting equipment.
5. Personal Protection: Use appropriate personal protective equipment during operation. In case of accidental contact with skin or eyes, rinse immediately with plenty of water and seek medical attention if necessary.
6. Biohazard: All blood-contaminated samples, containers, and waste reagents must be disposed of according to biohazard waste regulations, such as via autoclaving or professional treatment.
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