Thermostable RNase T1, CAS No.9026-12-4

CAS: 9026-12-4 Cat. No.: R749974 Molecular Weight: 11 kDa EC Number: 232-809-1

GRADE & PURITY Suitable for molecular biology, BioReagent, EnzymoPure™, DNase free, ≥95%(SDS-PAGE), 500 U/μL

Overview

R749974	Component	100KU	500KU	Storage
R749974A	Thermostable RNase T1	200 μL	5×200 μL	-20°C. Avoid freeze/thaw cycle
R749974B	10× RNase T1 Buffer	1 mL	5×1 mL	-20°C. Avoid freeze/thaw cycle

Performance description: 
Thermostable RNase T1 is a heat-resistant mutant based on RNase T1 through protein-directed evolution. Compared to wild-type RNase T1, ThermostableRNase T1 is able to maintain 100% activity at 50°C, while wild-type activity decreases by at least 50% at the same temperature. The higher reaction temperature helps to fully open the secondary structure of RNA and reduce or avoid its influence on enzyme digestion, thus making the enzyme digestion process more thorough.
Activity definition:
The activity unit (U) refers to the amount of enzyme required to change A260 by 1.0 using yeast RNA as substrate at 37°C and pH7.5.
Application: 
1. Remove RNA from DNA; 
2. Remove RNA from recombinant protein; 
3. RNA sequencing; 
4. For ribonuclease protection test (in combination with RNase A)
5. The transcription levels of DNA templates with no or low G content were measured.
Attention:
1. The thermal deactivation of Thermostable RNase T1 is reversible, and it is recommended to remove Thermostable RNase T1 by column purification or phenol/chloroform extraction.
2. Rnase-related experiments should be conducted in fume hoods or specific areas to avoid influence on other experiments.
3. For your safety and health, please wear a lab coat, disposable gloves and a mask to perform the experiment.

Specifications

Product Name

Thermostable RNase T1, CAS No.9026-12-4

Synonyms

Thermostable Ribonuclease T1 | Ribonuclease T1

Grade

BioReagent, DNase free, Suitable for molecular biology, EnzymoPure™

Specifications & Purity

BioReagent, DNase free, Suitable for molecular biology, EnzymoPure™, ≥95%(SDS-PAGE), 500 U/μL

CAS

9026-12-4

Molecule Type

Enzyme

Storage and Shipping

Concentration

500 U/μL

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Unit definition

One unit of the enzyme causes an increase in absorbance of 1.0 at 260nm in 15min when yeast RNA is hydrolyzed at 37℃ and pH7.5.