Thymidine (NSC 21548) - Moligand™, 10mM in DMSO , CAS No.50-89-5

CAS: 50-89-5 Cat. No.: T407939 Molecular Weight: 242.23 Beilstein Registry Number: 89285 EC Number: 200-070-4
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GRADE & PURITY Moligand™ ? Moligand™ — Aladdin's line of ligands and bioactive small molecules. Use for receptor, pathway, and binding studies needing defined small-molecule tools. 10mM in DMSO
Storage
Store at -80°C
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Dry ice packs + Cold packs
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T407939-1ml
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Why this grade

Moligand™, 10mM in DMSO Moligand™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

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Storage & shipping

Store at -80°C Ships Dry ice packs + Cold packs Check lot-specific COA for exact specifications.

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Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

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Literature proof

Cited in 34 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Information

Thymidine (Deoxythymidine, 2'-Deoxythymidine, 5-Methyldeoxyuridine, DThyd, NSC 21548) is a pyrimidine nucleoside that is composed of the pyrimidine base thymine attached to the sugar deoxyribose. As a constituent of DNA, thymidine pairs with adenine in th
In vitro


In vivo


Cell Data

cell lines:

Concentrations:

Incubation Time:

Powder Purity:

Specifications

Specifications & Purity
Moligand™, 10mM in DMSO
Biochemical and Physiological Mechanisms
Pyrimidine deoxynucleoside. Potent thymidine kinase substrate (IC 50 = 16 nM). Pairs with deoxyadenosine in double-stranded DNA. Synchronizes cells in S phase in vivo.
Storage
Store at -80°C
Shipped In
Dry ice packs + Cold packs
This product requires cold chain shipping. Ground and other economy services are not available.
Grade
Moligand™
Names and Identifiers
Isomeric SMILES CC1=CN(C(=O)NC1=O)[C@H]2C[C@@H]([C@H](O2)CO)O
WGK Germany 3
RTECS XP2071000
Molecular Weight 242.23
Beilstein 89285
Reaxy-Rn 889638
Reaxys-RN_link_address https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=889638&ln=

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

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✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

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📊 Datasheet

Quick-reference summary of product specifications and applications.

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🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

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Advanced Data

Associated Targets(Human)
TK1 Tchem Thymidine kinase, cytosolic (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Chemical and Physical Properties
Specific Rotation[α]33 ° (C=1, 1mol/L NaOH)
Melt Point(°C)183-191°C
Documents & Articles
Mechanisms of Action, Research Applications, and Cold-Induced Effects of Colchicine: An Overview
Pyrimidine Research Selection Guide: Structural Features, Typical Applications, and a Reagent/Building-Block Classification Navigator (Tables 1–5)
Functional Systems of Nucleoside Metabolism-Related Enzymes in Drug Response and Disease Biology
The Methodological Value of TPS-Cl: The Logic of Selective Activation and Representative Transformations in Nucleoside and Nucleotide Chemistry
Coupling Activators, Nucleoside Building Blocks, and Modification Design in Oligonucleotide Chemical Synthesis: Their Effects on Synthetic Efficiency, Purity, and Product Properties
Porous Graphitic Carbon (PGC) Chromatography Columns: Creating New Selectivity for Challenging Separations through Molecular Shape and the π-Electron Cloud on the Graphitic Surface
Citations of This Product
References
1. Yating Li, Jason Tan, Yanqin Tu, Jingnan Wu, Zaifang Zhang, Yifeng Wei, Xinan Jiao, Yan Zhou.  (2025)  Distinct Phage-Encoded Enzymes for Substitution of Deoxythymidine by Deoxyuridine in Phage Genomes.  Advanced Science,      [PMID:41001765] [10.1002/advs.202512937]
2. Yansheng Liu, Haoteng Wang, Guofu Wang, Xiaobo Jia, Jin Zhou, Hongqi Li, Haixin Chang, Zhicheng Jin, Kang Li, Zhaoxu Li.  (2025)  Real-time detection of mercury ions in environmental water conditions by using a differential phase SPR system equipped with a repeatable SPR chip.  MICROCHEMICAL JOURNAL,      [PMID:] [10.1016/j.microc.2025.115312]
3. Xu Lian, Li Hui-Min, Lin Juan.  (2023)  Efficient synthesis of 2’-deoxyguanosine in one-pot cascade by employing an engineered purine nucleoside phosphorylase from Brevibacterium acetylicum.  WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,  39  (10): (1-9).  [PMID:37606812] [10.1007/s11274-023-03721-1]
4. Fei Wang, Fan Yang, Jiawei Liu, Quan Bai.  (2023)  Studies on the retention mechanism of solutes in hydrophilic interaction chromatography using stoichiometric displacement theory II. HILIC/RPLC dual-retention mechanism of solutes in hydrophilic interaction chromatography over the entire range of water concentration in mobile phase.  TALANTA,      [PMID:37385194] [10.1016/j.talanta.2023.124858]
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9. Yan Wu, Kaixing Luo, Yanjuan Liu, Wei Chen, Zhengwu Bai, Sheng Tang.  (2022)  Innovative preparation of ureido/dodecyl dual-functionalized silica as a versatile mixed-mode stationary phase for high-resolution chromatographic separations.  JOURNAL OF CHROMATOGRAPHY A,      [PMID:35085896] [10.1016/j.chroma.2022.462834]
10. Chao Fan, Bei Liu, Hui Li, Kaijun Quan, Jia Chen, Hongdeng Qiu.  (2021)  N-Vinyl pyrrolidone and undecylenic acid copolymerized on silica surface as mixed-mode stationary phases for reversed-phase and hydrophilic interaction chromatography.  JOURNAL OF CHROMATOGRAPHY A,      [PMID:34509123] [10.1016/j.chroma.2021.462534]
11. Jia Ding, Fei Liu, Chunling Qi, Yunlei Zhou, Huanshun Yin, Shiyun Ai.  (2021)  Enhanced photoactivity of perovskite Bi4NbO8Cl/PTC-NH2 heterojunction and its application for photoelectrochemical sensing of DNA hydroxymethylation.  SENSORS AND ACTUATORS B-CHEMICAL,      [PMID:] [10.1016/j.snb.2021.130211]
12. Qian Wang, Huanshun Yin, Yunlei Zhou, Jun Wang, Shiyun Ai.  (2021)  Photoelectrochemical biosensor for 5-formylcytosine deoxyribonucleoside detection based on BiIO4-WS2/CuO ternary heterojunction.  SENSORS AND ACTUATORS B-CHEMICAL,      [PMID:] [10.1016/j.snb.2021.130019]
13. Tong Chen, Liang Xu, Guangsan Song, Yuan Li, Hong Xu, Hongbin Zhou, Zhen Xiao, Ping Li.  (2021)  Preparation and application of Au nanoparticles-decorated SO3H-cofunctionalized silica stationary phase for per aqueous liquid chromatography.  MICROCHEMICAL JOURNAL,      [PMID:] [10.1016/j.microc.2021.105985]
14. Chao Fan, Hao Tang, Licheng Wang, Yijing Li, Xusheng Wang, Shuai Wang, Xiaojing Liang.  (2020)  The preparation of a core–shell stationary phase by the in situ polymerization of a hydrophilic polymer on the surface of silica and its chromatographic performance.  NEW JOURNAL OF CHEMISTRY,  44  (27): (11704-11709).  [PMID:] [10.1039/D0NJ01197E]
15. Zhonghua Dong, Yueyue Sun, Guangwei Wei, Siying Li, Zhongxi Zhao.  (2019)  A Nucleoside/Nucleobase-Rich Extract from Cordyceps Sinensis Inhibits the Epithelial–Mesenchymal Transition and Protects against Renal Fibrosis in Diabetic Nephropathy.  MOLECULES,  24  (22): (4119).  [PMID:31739543] [10.3390/molecules24224119]
16. Tianpei Cai, Haijuan Zhang, Jia Chen, Zhan Li, Hongdeng Qiu.  (2019)  Polyethyleneimine-functionalized carbon dots and their precursor co-immobilized on silica for hydrophilic interaction chromatography.  JOURNAL OF CHROMATOGRAPHY A,      [PMID:30922720] [10.1016/j.chroma.2019.03.026]
17. Tengda Wang, Jianxing Shan, Li Wang, Xiangwen Zhang, Guozhu Li.  (2019)  On the kinetics of catalytic hydrogenation over Pd nanoparticles regulated by various nucleosides.  CHEMICAL ENGINEERING SCIENCE,      [PMID:] [10.1016/j.ces.2019.02.014]
18. Cai Tianpei, Zhang Haijuan, Rahman A. F. M. Mustafizur, Shi Yan-Ping, Qiu Hongdeng.  (2017)  Silica grafted with silanized carbon dots as a nano-on-micro packing material with enhanced hydrophilic selectivity.  MICROCHIMICA ACTA,  184  (8): (2629-2636).  [PMID:] [10.1007/s00604-017-2277-1]
19. Chaozhan Wang, Huanhuan Xu, Yinmao Wei.  (2015)  The preparation of high-capacity boronate affinity adsorbents by surface initiated reversible addition fragmentation chain transfer polymerization for the enrichment of ribonucleosides in serum.  ANALYTICA CHIMICA ACTA,      [PMID:26703260] [10.1016/j.aca.2015.11.013]
20. Qing Wang, Yao Long, Lin Yao, Li Xu, Zhi-Guo Shi, Lanying Xu.  (2015)  Preparation, characterization and application of a reversed phase liquid chromatography/hydrophilic interaction chromatography mixed-mode C18-DTT stationary phase.  TALANTA,      [PMID:26695288] [10.1016/j.talanta.2015.09.009]
21. Wang Xue-Jie, You Jin-Zong.  (2015)  Study on the molecular structure and thermal stability of pyrimidine nucleoside analogs.  JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY,  120  (1): (1009-1025).  [PMID:] [10.1007/s10973-014-4362-6]
22. Yali Yang, Jia Chen, Xiaojing Liang, Bei Liu, Kaijun Quan, Xiuhui Liu, Hongdeng Qiu.  (2024)  Adjustable chromatographic performance of silica-based mixed-mode stationary phase through the control of co-grafting amounts of imidazole and C18 chain.  JOURNAL OF CHROMATOGRAPHY A,      [PMID:38598894] [10.1016/j.chroma.2024.464889]
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24. Xingkang Diao, GuoHua Qi, Xinli Li, Yu Tian, Jing Li, Yongdong Jin.  (2025)  Label-Free Exosomal SERS Detection Assisted by Machine Learning for Accurately Discriminating Cell Cycle Stages and Revealing the Molecular Mechanisms during the Mitotic Process.  ANALYTICAL CHEMISTRY,      [PMID:39999424] [10.1021/acs.analchem.4c06240]
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